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The map label for this gene is yoaE [H]

Identifier: 187930174

GI number: 187930174

Start: 3245502

End: 3247598

Strand: Reverse

Name: yoaE [H]

Synonym: Rpic_3106

Alternate gene names: 187930174

Gene position: 3247598-3245502 (Counterclockwise)

Preceding gene: 187930175

Following gene: 187930173

Centisome position: 82.37

GC content: 67.53

Gene sequence:

>2097_bases
ATGTCCACCCAAGTCATCCGTGCCGCCTGCCCGCACGACTGCCCCGACACCTGCGCCTTGCTCGTCACCGTCGAGGACGG
CCGCGCCACCCGCGTGCAGGGCGACCCGGACCACCCGACGACTGCCGGCGTGCTGTGCACCAAGGTCAATCGGTATGCAG
AACGCACGTACCACCCGAACCGTCTGCTGACGCCGATGAAGCGCGTGGGCGCCAAGGGCGAGGGCAAGTTCGAACCGATC
TCGTGGGGCGAGGCCATCGACACCATCGCCACGCGTCTGGGGGACATTGCCGCGCGCAACCCGGAGGCCATCCTCCCGTA
CAGCTACGCCGGCACGATGGGCCTGGTGCAGGGGGAAAGCATGGCCGCGCGCTTCTTCCACAAGCTCGGAGCGTCGCTGC
TCGATCGCACGATCTGCGCATCGGCCGGGGCAACGGCGCTGCGCTACACGTATGGCGCGAGCCTCGGCATGGACATCGAG
CACGTGCAGGACGCCAAGCTGATCCTGATCTGGGGCGCCAATCCGATCGCATCGAACCTGCATTTCTGGACGCGCGCGCA
GGAAGCCAAACGGCGCGGCGCCACGCTCGTCGCCATCGATCCGTATCGCTCGCTGACGGCCGAGAAGTGCCATCGCCATC
TGGCGGTGCGCCCTGGTACCGATGCGGCGCTGGCGCTGGCGATGATCCACGTGTTGATCCGCGATGACCTGCTCGACCAC
GACTACATCGCCAATCACACGCTCGGTTTCGAGGCGCTCCGTGAACGCGCACAGCAATACACGCCGGAATATGCGGCGAC
GCTGTGTGGGGTGGACGCGGCGGATGTCGAGTGGCTCGCCAAGCTGTACGGCACGACCGTCGTGCGCGACAAGCAGCCGG
CGGCCATTCGCCTGAACTACGGCATGCAGCGCGCGCATGGCGGCGGGCAGGGCGTGCGCGCGGTCGCATGCCTGCCGTCG
CTGGTGGGCGCGTGGCGGGATGCAGCGGGCGGCCTGCAACTATCGACGTCCGGTTTCTTCCCCATCGACAATGCGAAGCT
GCAGCGTCCTGACCTGCTGCCGAGCTGGCCGGCGCTGCCCCGCACGATCAACATGAGCACCATCGGCGATGCGCTGCTGC
ATCCGGGCGATGCGTCGTTTGGCCCAAAGGTCGAGGCGGTGGTTGTCTACAACAGCAACCCCGTGGCGGTGGCGCCGGAT
TCGCGCAAGGTCGCCGCCGGCTTTGCGCGCGAAGACCTCTTCACCGTGGTGCTGGAGCATTTCCAGACTGACACCGCCGA
CTACGCCGACATCCTCCTGCCCGCCACCACGCAGCTCGAACATGTCGATGTGCACAAGGCGTATGGTCACACCTACGTGC
TGGCCAACAACGCTGCCATCGCGCCCATGGGGCAGTCGTTGCCGAACACGGAGATCTTCCGTCGCATTGCCCGGCGCATG
GGTTTTGACGACGCCTGCTTTGCCGACTCGGACGACGACATCGCCAGCCAGGCCGTGCGCCGTGACGACCCGGCCGCCGC
GCACATCGACTGGGCCACGCTCAAGCGCGAGGGCTGGCAGAAGCTGGCGCACCCCGCCGCGCCGCTGGCTCGTGGCGGCT
ATCGCACGCCGTCGGGCCGCTGCGAGTTCTATTCCGAGCAGATGGCCAAGGACGGCCTTGACCCGCTGCCCGGCTATGTG
CCGCCGCATGAGTCGGCGATCAGCACGCCTGAGCTGGCGAAGCGTTATCCGCTGTCGATGATCTCGCCGCCGGCGCGCAA
CTTCCTGAACTCGACCTTCGTCAACGTCGACAGCCTGCGCAGTACCGAGGGCGAGCCGCATCTCGATATCCACCCCGACG
ACGCGAATGCACGCGGCGTTGTCGACGGCGAGCTTGTCCGCATCTTCAATGATCGCGGCACCATGCAGGCGCGCGCCCGC
GTGACCGACCGTGCGCGGCGCGGCGTGGTCGTCGGCCTGTCGATCTGGTGGAAGAAGCTCGCGCCCGACGGCACCAACGC
CAACGAGCTGACCAGCCAGCGCCTGACCGACATGGGGCGTGCGCCCACCTTCTACGATTGCCTGGTCGAGGTCGCGCCGT
TGGCACAGGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGCTGCGAAGCACTTAGCAGGCGATTACAATTTCCGGATACTCGCGGCGGTGCGACAACGCCGACATGCACCCGCCGCC
ACCCACTCCGGAGCCCCCTG

Downstream 100 bases:

>100_bases
CGGAACCATCATGCGTGCCGCACGCGCGCTGTACCTTGCGCTGTCGCTTGCCGGTTCGGCGTTGCTGGCCGGCTGCGACA
CCGTCGGTTATTACTACCAG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MSTQVIRAACPHDCPDTCALLVTVEDGRATRVQGDPDHPTTAGVLCTKVNRYAERTYHPNRLLTPMKRVGAKGEGKFEPI
SWGEAIDTIATRLGDIAARNPEAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIE
HVQDAKLILIWGANPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
DYIANHTLGFEALRERAQQYTPEYAATLCGVDAADVEWLAKLYGTTVVRDKQPAAIRLNYGMQRAHGGGQGVRAVACLPS
LVGAWRDAAGGLQLSTSGFFPIDNAKLQRPDLLPSWPALPRTINMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPD
SRKVAAGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHVDVHKAYGHTYVLANNAAIAPMGQSLPNTEIFRRIARRM
GFDDACFADSDDDIASQAVRRDDPAAAHIDWATLKREGWQKLAHPAAPLARGGYRTPSGRCEFYSEQMAKDGLDPLPGYV
PPHESAISTPELAKRYPLSMISPPARNFLNSTFVNVDSLRSTEGEPHLDIHPDDANARGVVDGELVRIFNDRGTMQARAR
VTDRARRGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPLAQAA

Sequences:

>Translated_698_residues
MSTQVIRAACPHDCPDTCALLVTVEDGRATRVQGDPDHPTTAGVLCTKVNRYAERTYHPNRLLTPMKRVGAKGEGKFEPI
SWGEAIDTIATRLGDIAARNPEAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIE
HVQDAKLILIWGANPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
DYIANHTLGFEALRERAQQYTPEYAATLCGVDAADVEWLAKLYGTTVVRDKQPAAIRLNYGMQRAHGGGQGVRAVACLPS
LVGAWRDAAGGLQLSTSGFFPIDNAKLQRPDLLPSWPALPRTINMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPD
SRKVAAGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHVDVHKAYGHTYVLANNAAIAPMGQSLPNTEIFRRIARRM
GFDDACFADSDDDIASQAVRRDDPAAAHIDWATLKREGWQKLAHPAAPLARGGYRTPSGRCEFYSEQMAKDGLDPLPGYV
PPHESAISTPELAKRYPLSMISPPARNFLNSTFVNVDSLRSTEGEPHLDIHPDDANARGVVDGELVRIFNDRGTMQARAR
VTDRARRGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPLAQAA
>Mature_697_residues
STQVIRAACPHDCPDTCALLVTVEDGRATRVQGDPDHPTTAGVLCTKVNRYAERTYHPNRLLTPMKRVGAKGEGKFEPIS
WGEAIDTIATRLGDIAARNPEAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEH
VQDAKLILIWGANPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDHD
YIANHTLGFEALRERAQQYTPEYAATLCGVDAADVEWLAKLYGTTVVRDKQPAAIRLNYGMQRAHGGGQGVRAVACLPSL
VGAWRDAAGGLQLSTSGFFPIDNAKLQRPDLLPSWPALPRTINMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPDS
RKVAAGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHVDVHKAYGHTYVLANNAAIAPMGQSLPNTEIFRRIARRMG
FDDACFADSDDDIASQAVRRDDPAAAHIDWATLKREGWQKLAHPAAPLARGGYRTPSGRCEFYSEQMAKDGLDPLPGYVP
PHESAISTPELAKRYPLSMISPPARNFLNSTFVNVDSLRSTEGEPHLDIHPDDANARGVVDGELVRIFNDRGTMQARARV
TDRARRGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPLAQAA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=776, Percent_Identity=28.6082474226804, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1787870, Length=778, Percent_Identity=27.5064267352185, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI171474008, Length=775, Percent_Identity=26.4516129032258, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI3868721, Length=730, Percent_Identity=24.9315068493151, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787231, Length=749, Percent_Identity=24.4325767690254, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI145693196, Length=674, Percent_Identity=24.9258160237389, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081994, Length=719, Percent_Identity=22.6703755215577, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788534, Length=312, Percent_Identity=21.7948717948718, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI3868719, Length=570, Percent_Identity=21.7543859649123, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI3868720, Length=407, Percent_Identity=23.8329238329238, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787477, Length=298, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75960; Mature: 75829

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQVIRAACPHDCPDTCALLVTVEDGRATRVQGDPDHPTTAGVLCTKVNRYAERTYHPN
CCCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
RLLTPMKRVGAKGEGKFEPISWGEAIDTIATRLGDIAARNPEAILPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEHVQDAKLILIWGANPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCHHHCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCC
DYIANHTLGFEALRERAQQYTPEYAATLCGVDAADVEWLAKLYGTTVVRDKQPAAIRLNY
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEEECC
GMQRAHGGGQGVRAVACLPSLVGAWRDAAGGLQLSTSGFFPIDNAKLQRPDLLPSWPALP
CHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
RTINMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPDSRKVAAGFAREDLFTVVLEH
CEECHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHH
FQTDTADYADILLPATTQLEHVDVHKAYGHTYVLANNAAIAPMGQSLPNTEIFRRIARRM
HCCCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCEECCCCCCCCCHHHHHHHHHHC
GFDDACFADSDDDIASQAVRRDDPAAAHIDWATLKREGWQKLAHPAAPLARGGYRTPSGR
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
CEFYSEQMAKDGLDPLPGYVPPHESAISTPELAKRYPLSMISPPARNFLNSTFVNVDSLR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCHHHHHHHHHHCCHHHHH
STEGEPHLDIHPDDANARGVVDGELVRIFNDRGTMQARARVTDRARRGVVVGLSIWWKKL
CCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHH
APDGTNANELTSQRLTDMGRAPTFYDCLVEVAPLAQAA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STQVIRAACPHDCPDTCALLVTVEDGRATRVQGDPDHPTTAGVLCTKVNRYAERTYHPN
CCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
RLLTPMKRVGAKGEGKFEPISWGEAIDTIATRLGDIAARNPEAILPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEHVQDAKLILIWGANPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCHHHCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCC
DYIANHTLGFEALRERAQQYTPEYAATLCGVDAADVEWLAKLYGTTVVRDKQPAAIRLNY
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEEECC
GMQRAHGGGQGVRAVACLPSLVGAWRDAAGGLQLSTSGFFPIDNAKLQRPDLLPSWPALP
CHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
RTINMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPDSRKVAAGFAREDLFTVVLEH
CEECHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHH
FQTDTADYADILLPATTQLEHVDVHKAYGHTYVLANNAAIAPMGQSLPNTEIFRRIARRM
HCCCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCEECCCCCCCCCHHHHHHHHHHC
GFDDACFADSDDDIASQAVRRDDPAAAHIDWATLKREGWQKLAHPAAPLARGGYRTPSGR
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
CEFYSEQMAKDGLDPLPGYVPPHESAISTPELAKRYPLSMISPPARNFLNSTFVNVDSLR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCHHHHHHHHHHCCHHHHH
STEGEPHLDIHPDDANARGVVDGELVRIFNDRGTMQARARVTDRARRGVVVGLSIWWKKL
CCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHH
APDGTNANELTSQRLTDMGRAPTFYDCLVEVAPLAQAA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]