Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 187930159

GI number: 187930159

Start: 3227910

End: 3229550

Strand: Reverse

Name: murD [H]

Synonym: Rpic_3091

Alternate gene names: 187930159

Gene position: 3229550-3227910 (Counterclockwise)

Preceding gene: 187930160

Following gene: 187930158

Centisome position: 81.92

GC content: 68.74

Gene sequence:

>1641_bases
ATGACCGACATGACCGACGACTCCATCACCCCGGACGACGATATGGCCGCCACGCAGCCCGACGCCGCCGTGGACGCCGT
TGTCGCGACACTCGATTCGGCCGAGCCGATGCCCGCCGCTGAAACCGCACAGGCGCCGCGCATGTTCGGCGAGTTCGAAG
CGCCGGTGGTGCTTGTGCTCGGCCTCGGCGAATCCGGCCTCGCCATGGCACGCTGGTGTGCCCGTCATGGCGCGCCCGTG
CGCGTGGCCGACACGCGCGAGGCACCGGCCAACCTGCCGGTGCTGCGCGCCCATGTGCCGGACGCGGAGTTTGTGACCGG
TCCCTTTGCCGTGTCGCTGCTGGACGGTGTGACGTTGGTGGCGATCAGCCCTGGCTTGTCGCCGCTGGATCCGAACGTCG
CGGCGCTGCTGAACGCCGCCAATGCGCAGAGCGTGCCGGTGTGGGGCGAGATCGAATTGTTTGCCCGCGCGCTGGCCGGC
CTCAAGGCCGCGCAAGGCTATGCGCCGCGCGTGCTCGCCATCACGGGCACGAACGGGAAGACCACCACCACGGCGCTCAC
GGGCGCCCTGGTCCAGCGCGCCGGCAAGACGGTCGGCGTGGCCGGCAACATCAGCCCGTCGGCGCTGGACAAGCTGAGCG
AATGCGTCGACGCAGGCACGCTGCCTGACGTGTGGGTGCTCGAGCTTTCCAGCTTCCAGCTCGAAACCACGCATTCGCTG
GAAGCCGATGCGGCCACCGTACTGAACATCACGCAGGATCACCTCGACTGGCACGGCACCATGGAGGCCTACGCCGCAGC
GAAGGGCCGCATCTTCGGTGCCAACACCGTGCGCGTGCTGAACCGTCAGGACGCGGCCGTGATGGCATTCGCGAGCAAGA
ACGGCGGCGATATCACCTTCGGCATCGATGAGCCGGGCACGCCCGATGCGCTCGGCCTGCTGCGCGATGGCGGCATGCCC
TGGATCGTATTGGCCGAAGTCGAAGAGGACGATCTGCCCAAGCCTACGCGCCGCAAGAAGGGCGACGCCGCGCCGGCCGC
GCCGGTGCCGGTGCGCCTCAAGCGTCTGATGCCGGCCGACGCACTGCGCATCCGTGGCTTGCACAACGCGACCAACGCGA
TGGCTGCGCTGGCACTGTGTCGCGCGATCGGCCTGCCAGTGAGCGCGCTGCTGCACGGCCTGCGCGACTACGCCGGCGAG
CCGCACCGCGTCGAACTGATCGCCGCGTTTGACGACATCGAATTCTTCGACGACAGCAAGGGCACCAACGTCGGCGCGAC
GGTGGCTGCACTCTCGGGCCTGTCCAAGCATGTCGTGCTGATCGCCGGTGGCGATGGCAAGGGTCAGGACTTTTCGCCGC
TGGCCGAGCCGGTCGCGCAGTACGCGCGCGCCGTGATCCTCATCGGTCGCGATGCACCGCTGATTCGTGAGGCGCTGGCC
AATACCGGTGTCGCGCTGGTCGACGCGCCGACGCTGGAAGCCGCCGTGCTGGAAGCCGCCGCGCATGCGCAGCCGGGCGA
CGCCGTGCTGTTGTCGCCGGCGTGCGCAAGCTTCGACATGTTCCGCAACTACGAACACCGCGCCCAGGTGTTCCACGAGG
CCGTGGTCGCATTGGCGGCAGACCGAGGAGTCCTCCTATGA

Upstream 100 bases:

>100_bases
CCGGCTGGAAGGAAACGCAAGTGGTCGTGCGCTTCTGGGTCATCACCATGATGCTGGTGCTGATCGGCCTGTCGACCCTG
AAGCTGCGGTGAGCACGGAC

Downstream 100 bases:

>100_bases
GCGACACCCAGATCAAGCGCAGCGGCTTCATCGGCGCGACCATCGGCAATGCGTGGGATGGCCTGCGCGACGCCGTTTCA
GGGGTCAAGCCCACGCGCTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTDMTDDSITPDDDMAATQPDAAVDAVVATLDSAEPMPAAETAQAPRMFGEFEAPVVLVLGLGESGLAMARWCARHGAPV
RVADTREAPANLPVLRAHVPDAEFVTGPFAVSLLDGVTLVAISPGLSPLDPNVAALLNAANAQSVPVWGEIELFARALAG
LKAAQGYAPRVLAITGTNGKTTTTALTGALVQRAGKTVGVAGNISPSALDKLSECVDAGTLPDVWVLELSSFQLETTHSL
EADAATVLNITQDHLDWHGTMEAYAAAKGRIFGANTVRVLNRQDAAVMAFASKNGGDITFGIDEPGTPDALGLLRDGGMP
WIVLAEVEEDDLPKPTRRKKGDAAPAAPVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGE
PHRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVILIGRDAPLIREALA
NTGVALVDAPTLEAAVLEAAAHAQPGDAVLLSPACASFDMFRNYEHRAQVFHEAVVALAADRGVLL

Sequences:

>Translated_546_residues
MTDMTDDSITPDDDMAATQPDAAVDAVVATLDSAEPMPAAETAQAPRMFGEFEAPVVLVLGLGESGLAMARWCARHGAPV
RVADTREAPANLPVLRAHVPDAEFVTGPFAVSLLDGVTLVAISPGLSPLDPNVAALLNAANAQSVPVWGEIELFARALAG
LKAAQGYAPRVLAITGTNGKTTTTALTGALVQRAGKTVGVAGNISPSALDKLSECVDAGTLPDVWVLELSSFQLETTHSL
EADAATVLNITQDHLDWHGTMEAYAAAKGRIFGANTVRVLNRQDAAVMAFASKNGGDITFGIDEPGTPDALGLLRDGGMP
WIVLAEVEEDDLPKPTRRKKGDAAPAAPVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGE
PHRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVILIGRDAPLIREALA
NTGVALVDAPTLEAAVLEAAAHAQPGDAVLLSPACASFDMFRNYEHRAQVFHEAVVALAADRGVLL
>Mature_545_residues
TDMTDDSITPDDDMAATQPDAAVDAVVATLDSAEPMPAAETAQAPRMFGEFEAPVVLVLGLGESGLAMARWCARHGAPVR
VADTREAPANLPVLRAHVPDAEFVTGPFAVSLLDGVTLVAISPGLSPLDPNVAALLNAANAQSVPVWGEIELFARALAGL
KAAQGYAPRVLAITGTNGKTTTTALTGALVQRAGKTVGVAGNISPSALDKLSECVDAGTLPDVWVLELSSFQLETTHSLE
ADAATVLNITQDHLDWHGTMEAYAAAKGRIFGANTVRVLNRQDAAVMAFASKNGGDITFGIDEPGTPDALGLLRDGGMPW
IVLAEVEEDDLPKPTRRKKGDAAPAAPVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGEP
HRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVILIGRDAPLIREALAN
TGVALVDAPTLEAAVLEAAAHAQPGDAVLLSPACASFDMFRNYEHRAQVFHEAVVALAADRGVLL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=491, Percent_Identity=37.2708757637475, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 56768; Mature: 56637

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMTDDSITPDDDMAATQPDAAVDAVVATLDSAEPMPAAETAQAPRMFGEFEAPVVLVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCCCEEEEE
GLGESGLAMARWCARHGAPVRVADTREAPANLPVLRAHVPDAEFVTGPFAVSLLDGVTLV
ECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCEEEE
AISPGLSPLDPNVAALLNAANAQSVPVWGEIELFARALAGLKAAQGYAPRVLAITGTNGK
EECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
TTTTALTGALVQRAGKTVGVAGNISPSALDKLSECVDAGTLPDVWVLELSSFQLETTHSL
CHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
EADAATVLNITQDHLDWHGTMEAYAAAKGRIFGANTVRVLNRQDAAVMAFASKNGGDITF
CCCCHHEEECHHHHCCCCCHHHHHHHHCCEEECCCEEEEECCCCCEEEEEECCCCCEEEE
GIDEPGTPDALGLLRDGGMPWIVLAEVEEDDLPKPTRRKKGDAAPAAPVPVRLKRLMPAD
CCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCHHHHHHCCHH
ALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGEPHRVELIAAFDDIEFFDDSK
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCC
GTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVILIGRDAPLIREALA
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHH
NTGVALVDAPTLEAAVLEAAAHAQPGDAVLLSPACASFDMFRNYEHRAQVFHEAVVALAA
HCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHC
DRGVLL
CCCCCC
>Mature Secondary Structure 
TDMTDDSITPDDDMAATQPDAAVDAVVATLDSAEPMPAAETAQAPRMFGEFEAPVVLVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCCCEEEEE
GLGESGLAMARWCARHGAPVRVADTREAPANLPVLRAHVPDAEFVTGPFAVSLLDGVTLV
ECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCEEEE
AISPGLSPLDPNVAALLNAANAQSVPVWGEIELFARALAGLKAAQGYAPRVLAITGTNGK
EECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
TTTTALTGALVQRAGKTVGVAGNISPSALDKLSECVDAGTLPDVWVLELSSFQLETTHSL
CHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
EADAATVLNITQDHLDWHGTMEAYAAAKGRIFGANTVRVLNRQDAAVMAFASKNGGDITF
CCCCHHEEECHHHHCCCCCHHHHHHHHCCEEECCCEEEEECCCCCEEEEEECCCCCEEEE
GIDEPGTPDALGLLRDGGMPWIVLAEVEEDDLPKPTRRKKGDAAPAAPVPVRLKRLMPAD
CCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCHHHHHHCCHH
ALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGEPHRVELIAAFDDIEFFDDSK
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCC
GTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVILIGRDAPLIREALA
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHH
NTGVALVDAPTLEAAVLEAAAHAQPGDAVLLSPACASFDMFRNYEHRAQVFHEAVVALAA
HCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHC
DRGVLL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]