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The map label for this gene is gapA [H]

Identifier: 187930056

GI number: 187930056

Start: 3114332

End: 3115330

Strand: Reverse

Name: gapA [H]

Synonym: Rpic_2988

Alternate gene names: 187930056

Gene position: 3115330-3114332 (Counterclockwise)

Preceding gene: 187930057

Following gene: 187930055

Centisome position: 79.02

GC content: 62.26

Gene sequence:

>999_bases
ATGACCATCAAGATCGGCATCAACGGCTTCGGCCGCATCGGACGCATGGTGTTCCGCGCCGCCGCCGCCAACTTCAAGGA
CATCGAAGTTGTTGCCATCAACGACCTGCTCGAGCCCGACTACCTGGCGTACATGCTGAAGTACGACTCGGTGCACGGCC
GCTTCGACGGTGAAGTGTCGGTCGACGGCAACACGCTCGTCGTCAACGGCAAGAAGATCCGCCTGACCGCCGTGAAGGAC
CCGTCCGAGCTGAAGTGGGGCGAAGTCGGCGCCGACGTGGTGATCGAATCGACCGGCATCTTCCTGACCAAGGAAGGCGC
GCAGAAGCACATCGACGCGGGCGCCAAGAAGGTGATCATGTCGGCCCCGTCGAAGGACGACACCCCGATGTTCGTGTACG
GCGTGAACCACGAAACGTACAAGGGCGAGGCGATCATCTCCAACGCTTCGTGCACGACCAACTGCCTCGCACCGGTTGCC
AAGGTGCTGAACGACAAGTGGGGCATCAAGCGCGGCCTGATGACGACCGTGCACGCTGCCACTGCCACGCAGAAGACCGT
TGACGGCCCGTCCAACAAGGACTGGCGCGGCGGCCGCGGCATCCTGGAAAACATCATCCCGTCGTCCACTGGCGCCGCCA
AGGCCGTGGGCGTGGTGATTCCGCAGCTGAACAAGAAGCTGACCGGCATGTCGTTCCGCGTGCCGACCTCGGATGTGTCG
GTCGTCGACCTGACCGTCGAGCTGGAAAGCGCCGCCACGTACGCTGAAATCTGCGCCGAAATGAAGGCACAGAGCGAAGG
CCCGCTGAAGGGCGTGCTCGGCTACACCGAAGACAAGGTCGTCGCTACGGATTTCCGCGGCGATGCACGCACCTCCATCT
TCGACGCCGAAGCCGGCATCGCACTGGATGGCACCTTCATCAAGGTCGTGAGCTGGTACGACAACGAGTGGGGCTATTCG
AACAAGTGCCTGGAAATGGCACGTGTGGTGGGCAAGTAA

Upstream 100 bases:

>100_bases
GCGTCATTGCTTAACTGAATTTGCGCACGCGGCACCTACGAGGTGCCGCGCTGGTTTTTATCGCATCGTCGATTTCTTTT
CTGCTATCAGGAGACTGACC

Downstream 100 bases:

>100_bases
TCCAACCCACTTAGCAGTCGAAAGAGAACGCGCCTTCGGGCGCGTTTTTTCATAGGCCTGCGGTCCGGGTGAGGTGTCTC
CCCAAAACGTTGTCGCTTGG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PSELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELESAATYAEICAEMKAQSEGPLKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKCLEMARVVGK

Sequences:

>Translated_332_residues
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PSELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELESAATYAEICAEMKAQSEGPLKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKCLEMARVVGK
>Mature_331_residues
TIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKDP
SELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAK
VLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVSV
VDLTVELESAATYAEICAEMKAQSEGPLKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYSN
KCLEMARVVGK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=60.6606606606607, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=56.5349544072948, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=70.7831325301205, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=38.9057750759878, Blast_Score=248, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17534677, Length=331, Percent_Identity=61.3293051359517, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17534679, Length=331, Percent_Identity=61.3293051359517, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32566163, Length=330, Percent_Identity=60.9090909090909, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17568413, Length=330, Percent_Identity=60.9090909090909, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=62.0481927710843, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=61.4457831325301, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=61.144578313253, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17933600, Length=327, Percent_Identity=59.3272171253823, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI18110149, Length=327, Percent_Identity=59.3272171253823, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI85725000, Length=327, Percent_Identity=59.6330275229358, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI22023983, Length=327, Percent_Identity=59.6330275229358, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=60.8024691358025, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35707; Mature: 35576

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPSELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELESAATYAEICAEMKAQSEGPLKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKCLEMARVVGK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
EEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPSELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELESAATYAEICAEMKAQSEGPLKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKCLEMARVVGK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852; 8366033 [H]