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The map label for this gene is purB [H]

Identifier: 187930032

GI number: 187930032

Start: 3086879

End: 3088252

Strand: Reverse

Name: purB [H]

Synonym: Rpic_2964

Alternate gene names: 187930032

Gene position: 3088252-3086879 (Counterclockwise)

Preceding gene: 187930034

Following gene: 187930031

Centisome position: 78.33

GC content: 64.05

Gene sequence:

>1374_bases
ATGTCGTCGCTTTCTGCCCTGACCGCTCTGTCTCCCATCGATGGCCGCTACGCTGCCAAGGCCGATCCGCTGCGCGAGTG
GCTGTCGGAAGCCGCTTTCATGCGCAATCGCGTGAAGGTGGAGGTGCATTGGCTGATCGCCCTGTCGCAGGCTGGCCTGG
CAGAGATTCCGCGCTTCTCGGCCGCCGCCGAGGGCGCACTGCTGGCTCTGGTGGAGAACTTTGCCGAGGCAGACGCCGCC
CGCATCAAAGAGATCGAAGCCGTCACCAACCATGACGTGAAGGCCGTCGAGTACTGGATGAAGGAGCGCGTGCGCGGCAA
TGCCGAACTGGAAGCCGCCAGCGAGTTCATCCACTTCGCCTGCACGTCGGAAGACATCAATAACACCTCCCACGGGATGA
TGCTCAAGGGTGCGCGTGACACCGTGATCGTGCCGACGCTGCGCCGCGTTCAGGCTCGCCTGGTGGAGCTGGCTCATGCC
AACGCCGACGTGCCGATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACCACGCTGGGCAAGGAAATGGCCAACGT
GGCCGCGCGCCTGGACCGCGCCATCCGCCGCATCGAAGCCGTTGAACTGCTGGGCAAGATGAACGGCGCCGTGGGCAACT
ACAATGCGCACCTGTCGGCGTATCCGTCGCTGGACTGGGAAGCCTTCTCGAAGAACGTGATCGAGACGCGCCTGGGCCTG
ACGTTCAACCCGTACACCATCCAGATCGAGCCGCACGATTACATGGCGGAGCTGTTCGACGCCGTGGCGCGCGCCAACAC
GATCATCCTGGATCTGGACCGCGACGTCTGGGGCTACATCTCGCAGGGCTACTTCAAACAGAAGACCAAGGCCGGCGAGA
TCGGCTCGTCGACGATGCCACACAAGGTCAACCCGATCGACTTCGAAAACTCCGAAGGCAACGTCGGCCTGGCCAACGCG
GTGCTGCGCCATCTGTCGGAAAAGCTGCCGGTATCGCGTTGGCAGCGTGATCTGACCGATTCGACCGTGCTGCGCAACAT
CGGCGTGGCGTTCGGCTATAGCCTGCTCGCATACGACGCCTGCCTGCGCGGCCTCGGCAAGCTCGAAACCAACCCGGCCC
GTCTGGCCGAAGACCTCGACGCCTGCTGGGAAGTGCTGGCCGAGCCGGTGCAGACCGTGATGCGTCGCTACGGCATTGCC
AACCCGTACGAGCAACTCAAGGAACTGACGCGCGGCAAGGGCATTTCGCGAGATGCGCTGCGTGAGTTCATCGGCACGCT
GGCGATTCCCGAAGACGCTCGCAAGCTGCTGCTGGACATGACGCCCGGCAGCTATATCGGCAAGGCAGAAGAACTGGCGC
GCCGTATCGCCTGA

Upstream 100 bases:

>100_bases
GCGGGCTCGCTTGGGTGCGCATATGCACCTTGGTTCAACGCGCAACGCCGCGTCCGGGTTGTCCGGATTGAGATTCCCTT
CACATTTTTCCCCGCCTGCC

Downstream 100 bases:

>100_bases
TTGTTGCAAAAATTTGGTGGTGTGGTCTGAAGAACGGCCCGCTCTCGAATCCGAGGGCGGGCCGTTTTGGTTGGCGTCCT
CCGCAGAGCTATCCGCTGAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRNRVKVEVHWLIALSQAGLAEIPRFSAAAEGALLALVENFAEADAA
RIKEIEAVTNHDVKAVEYWMKERVRGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHA
NADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPSLDWEAFSKNVIETRLGL
TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANA
VLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIA
NPYEQLKELTRGKGISRDALREFIGTLAIPEDARKLLLDMTPGSYIGKAEELARRIA

Sequences:

>Translated_457_residues
MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRNRVKVEVHWLIALSQAGLAEIPRFSAAAEGALLALVENFAEADAA
RIKEIEAVTNHDVKAVEYWMKERVRGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHA
NADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPSLDWEAFSKNVIETRLGL
TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANA
VLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIA
NPYEQLKELTRGKGISRDALREFIGTLAIPEDARKLLLDMTPGSYIGKAEELARRIA
>Mature_456_residues
SSLSALTALSPIDGRYAAKADPLREWLSEAAFMRNRVKVEVHWLIALSQAGLAEIPRFSAAAEGALLALVENFAEADAAR
IKEIEAVTNHDVKAVEYWMKERVRGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHAN
ADVPMLSRTHGQPASPTTLGKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPSLDWEAFSKNVIETRLGLT
FNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANAV
LRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDACLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIAN
PYEQLKELTRGKGISRDALREFIGTLAIPEDARKLLLDMTPGSYIGKAEELARRIA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=447, Percent_Identity=63.0872483221477, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50529; Mature: 50398

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSALTALSPIDGRYAAKADPLREWLSEAAFMRNRVKVEVHWLIALSQAGLAEIPRFS
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHCCCHH
AAAEGALLALVENFAEADAARIKEIEAVTNHDVKAVEYWMKERVRGNAELEAASEFIHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHE
CTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHANADVPMLSRTHGQPASPTTL
ECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHH
GKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPSLDWEAFSKNVIETRLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMP
EECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
HKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIANPYEQLKELTRGKGISRDAL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
REFIGTLAIPEDARKLLLDMTPGSYIGKAEELARRIA
HHHHHHCCCCHHHHHHHHCCCCCHHCCCHHHHHHHCC
>Mature Secondary Structure 
SSLSALTALSPIDGRYAAKADPLREWLSEAAFMRNRVKVEVHWLIALSQAGLAEIPRFS
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHCCCHH
AAAEGALLALVENFAEADAARIKEIEAVTNHDVKAVEYWMKERVRGNAELEAASEFIHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHE
CTSEDINNTSHGMMLKGARDTVIVPTLRRVQARLVELAHANADVPMLSRTHGQPASPTTL
ECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHH
GKEMANVAARLDRAIRRIEAVELLGKMNGAVGNYNAHLSAYPSLDWEAFSKNVIETRLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
TFNPYTIQIEPHDYMAELFDAVARANTIILDLDRDVWGYISQGYFKQKTKAGEIGSSTMP
EECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
HKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLRGLGKLETNPARLAEDLDACWEVLAEPVQTVMRRYGIANPYEQLKELTRGKGISRDAL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
REFIGTLAIPEDARKLLLDMTPGSYIGKAEELARRIA
HHHHHHCCCCHHHHHHHHCCCCCHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]