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The map label for this gene is purN [H]

Identifier: 187929792

GI number: 187929792

Start: 2836192

End: 2836842

Strand: Reverse

Name: purN [H]

Synonym: Rpic_2722

Alternate gene names: 187929792

Gene position: 2836842-2836192 (Counterclockwise)

Preceding gene: 187929794

Following gene: 187929791

Centisome position: 71.95

GC content: 63.13

Gene sequence:

>651_bases
ATGAAAAACATCGTCATTCTCATTTCCGGACGCGGCTCCAATATGGAAGCCATTGTCCGCGCCTGTCAGGCCGAAGGTTG
GTCGGGACGCATTGCCGCCGTCATTTCTAATCGGCCCGATGCGGCAGGACTTAAATTTGCCGCTTCGCACGGCATTGCCA
CCGCGGTGGTCGACCACAAGGCTTTCCCCGATCGCGACAGCTTCGATGCCGCCCTGGCGCAGGTGATCGACGGCTTTTCG
CCGGATCTGGTGGTGCTGGCCGGTTTCATGCGCATCCTCACGCCCGGCTTTGTAAAGCGCTACGCCGGCCGCATGCTGAA
CATTCATCCGTCGCTGCTGCCGTGCTTCCCCGGGTTGCACACGCACGAGGCCGCGCTGGCCATGGGCGTGAAGGTGCATG
GCGCGACTGTACATTTCGTCACCGCCGACCTGGATCACGGCCCGATCGTGCTGCAGGCCATCATCGACGTGCGTCAAGGC
GACACGCCTGACTCGCTGGCCGGGCGGCTGCTGGCACAGGAGCACACGATTTACCCGCGTGCCGTGCGCTGGTTCGTGGA
AGGGCGGTTGTCGGTCGAACACGGTGTGGTGCGCGTCTCGCCGGATGAATCGCAGCTTGTCATTGGCGTCAACGCCCGGG
AGGCAGCATGA

Upstream 100 bases:

>100_bases
CAAAATGCCACGGTGACTGCAAAAGCGCCCGCGGCATGGCCTTGCAGCCGTCACACCCGATTTTCTCGGGGAACGGCGGC
GCACTTGATAAAATGCCGCG

Downstream 100 bases:

>100_bases
GCCAGAATCGTCAGCGTTCGGGCGGCAATGGAAGCGGCGGCGGGTCGGGCAACAAGCGCGCGGGTTCGGGTGGTGGCGGC
TGGTATCGGGCCCCGCAGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKNIVILISGRGSNMEAIVRACQAEGWSGRIAAVISNRPDAAGLKFAASHGIATAVVDHKAFPDRDSFDAALAQVIDGFS
PDLVVLAGFMRILTPGFVKRYAGRMLNIHPSLLPCFPGLHTHEAALAMGVKVHGATVHFVTADLDHGPIVLQAIIDVRQG
DTPDSLAGRLLAQEHTIYPRAVRWFVEGRLSVEHGVVRVSPDESQLVIGVNAREAA

Sequences:

>Translated_216_residues
MKNIVILISGRGSNMEAIVRACQAEGWSGRIAAVISNRPDAAGLKFAASHGIATAVVDHKAFPDRDSFDAALAQVIDGFS
PDLVVLAGFMRILTPGFVKRYAGRMLNIHPSLLPCFPGLHTHEAALAMGVKVHGATVHFVTADLDHGPIVLQAIIDVRQG
DTPDSLAGRLLAQEHTIYPRAVRWFVEGRLSVEHGVVRVSPDESQLVIGVNAREAA
>Mature_216_residues
MKNIVILISGRGSNMEAIVRACQAEGWSGRIAAVISNRPDAAGLKFAASHGIATAVVDHKAFPDRDSFDAALAQVIDGFS
PDLVVLAGFMRILTPGFVKRYAGRMLNIHPSLLPCFPGLHTHEAALAMGVKVHGATVHFVTADLDHGPIVLQAIIDVRQG
DTPDSLAGRLLAQEHTIYPRAVRWFVEGRLSVEHGVVRVSPDESQLVIGVNAREAA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=45.6410256410256, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=45.6410256410256, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=45.6410256410256, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=53.6842105263158, Blast_Score=213, Evalue=8e-57,
Organism=Escherichia coli, GI1787483, Length=199, Percent_Identity=35.1758793969849, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=42.4581005586592, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=202, Percent_Identity=30.6930693069307, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=42.8571428571429, Blast_Score=156, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23108; Mature: 23108

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVILISGRGSNMEAIVRACQAEGWSGRIAAVISNRPDAAGLKFAASHGIATAVVDHK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCC
AFPDRDSFDAALAQVIDGFSPDLVVLAGFMRILTPGFVKRYAGRMLNIHPSLLPCFPGLH
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCEEECCCCHHHCCCCCC
THEAALAMGVKVHGATVHFVTADLDHGPIVLQAIIDVRQGDTPDSLAGRLLAQEHTIYPR
CCHHHHHCCEEEECEEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
AVRWFVEGRLSVEHGVVRVSPDESQLVIGVNAREAA
HHHHHHHCCEEEECCEEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKNIVILISGRGSNMEAIVRACQAEGWSGRIAAVISNRPDAAGLKFAASHGIATAVVDHK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCC
AFPDRDSFDAALAQVIDGFSPDLVVLAGFMRILTPGFVKRYAGRMLNIHPSLLPCFPGLH
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCEEECCCCHHHCCCCCC
THEAALAMGVKVHGATVHFVTADLDHGPIVLQAIIDVRQGDTPDSLAGRLLAQEHTIYPR
CCHHHHHCCEEEECEEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
AVRWFVEGRLSVEHGVVRVSPDESQLVIGVNAREAA
HHHHHHHCCEEEECCEEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]