BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is paaF [H]

Identifier: 187929699

GI number: 187929699

Start: 2741476

End: 2742261

Strand: Reverse

Name: paaF [H]

Synonym: Rpic_2628

Alternate gene names: 187929699

Gene position: 2742261-2741476 (Counterclockwise)

Preceding gene: 187929700

Following gene: 187929698

Centisome position: 69.56

GC content: 65.52

Gene sequence:

>786_bases
ATGAGCGAAGCCAGCGAAGTGCTCGTCAGCCGCGATGGCGCCATCGCCACCTTGACCATCAACCGCCCGCGCGCGAAGAA
CAGCTTGAACCGCGCGGTATTCGATGGCCTGCGTGCGCAGCTTGTGCAACTGCGCGACGACGATACCGTGCGCGCGGTCA
TCATCACCGGCGCCGAGGGCGTGTTCTGCGCCGGGGCCGACATCACGGCCTTCGATGCGCTGCGCGCCGAGCCACTGCTG
GGCGATCGTGCGGCAGCAGGCGGCCACTTTTGGTCGGAACTGGAGCGCTTCCCCAAGCCCGTCATCGCAGCGGTGGAAAA
GCTCGCGCTGGGCGGCGGCACAGAATTAGCGCTGGCCTGCGACATCGCGATCGCTGGCGAGTCTGCCAATTTTGGTGTGC
CGGAAGTCAAGCTGGGCGCCATTCCGGGTGCCGGGGGCACGCAGCGCCTGATCCACGCTATCGGTAAGTCCAAGGCCATG
GCCCTGTTGCTGACCGGCGATTTCATCGACGCCCGCAAGGCTTGCGATGCAGGCATGGTCGCCGAAGTGACGGCTGATGG
CCAGGCCCTGCCGACGGCGTTGGCGATGGCCAACCGGATTGCAGCCAATTCGCCGCTGGCCGTGGCGCTGGCGAAAGATG
CGGCACTGGCCAGCTTTGAGACTCCGCTCGCGCAAGGCCTGGAGCATGAGAAGCGTAATTTCCTCGTCGCTTTGCGTTCG
GCCGACAACCTGGAAGGGCAAGCGGCATTCCTGGGCAAGCGCACCCCCCACTTCACGGGCCAATAA

Upstream 100 bases:

>100_bases
TGGAGCGGCGGGGCGGCCGCTATGGCCTGCAGACCATCTGCGAGGCTTCGGGCACGGCCAATACCACCATCATCGAGCGG
CTTGGATGAGCGGAGCCACC

Downstream 100 bases:

>100_bases
CGCCTGTCTATCTGTCATCCATCGAGGAAAAACCATGCAACTGAACTCCGACATCTCGGCCATCATCACCGGGGGCGCTT
CCGGCCTGGGAGCCGCCACG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSEASEVLVSRDGAIATLTINRPRAKNSLNRAVFDGLRAQLVQLRDDDTVRAVIITGAEGVFCAGADITAFDALRAEPLL
GDRAAAGGHFWSELERFPKPVIAAVEKLALGGGTELALACDIAIAGESANFGVPEVKLGAIPGAGGTQRLIHAIGKSKAM
ALLLTGDFIDARKACDAGMVAEVTADGQALPTALAMANRIAANSPLAVALAKDAALASFETPLAQGLEHEKRNFLVALRS
ADNLEGQAAFLGKRTPHFTGQ

Sequences:

>Translated_261_residues
MSEASEVLVSRDGAIATLTINRPRAKNSLNRAVFDGLRAQLVQLRDDDTVRAVIITGAEGVFCAGADITAFDALRAEPLL
GDRAAAGGHFWSELERFPKPVIAAVEKLALGGGTELALACDIAIAGESANFGVPEVKLGAIPGAGGTQRLIHAIGKSKAM
ALLLTGDFIDARKACDAGMVAEVTADGQALPTALAMANRIAANSPLAVALAKDAALASFETPLAQGLEHEKRNFLVALRS
ADNLEGQAAFLGKRTPHFTGQ
>Mature_260_residues
SEASEVLVSRDGAIATLTINRPRAKNSLNRAVFDGLRAQLVQLRDDDTVRAVIITGAEGVFCAGADITAFDALRAEPLLG
DRAAAGGHFWSELERFPKPVIAAVEKLALGGGTELALACDIAIAGESANFGVPEVKLGAIPGAGGTQRLIHAIGKSKAMA
LLLTGDFIDARKACDAGMVAEVTADGQALPTALAMANRIAANSPLAVALAKDAALASFETPLAQGLEHEKRNFLVALRSA
DNLEGQAAFLGKRTPHFTGQ

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=40.2390438247012, Blast_Score=184, Evalue=5e-47,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=37.3015873015873, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI68989263, Length=262, Percent_Identity=32.4427480916031, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=33.7254901960784, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=35.2272727272727, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI70995211, Length=237, Percent_Identity=32.0675105485232, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=27.34375, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=26.2548262548263, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=26.2548262548263, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=26.2548262548263, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=24.7104247104247, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=24.7104247104247, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=24.7104247104247, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=24.7104247104247, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=231, Percent_Identity=25.974025974026, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=231, Percent_Identity=25.974025974026, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI157694520, Length=201, Percent_Identity=26.865671641791, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=43.1906614785992, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=32.7067669172932, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=37.9310344827586, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=36.6834170854271, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=29.8449612403101, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=28.6852589641434, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=32.9411764705882, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=41.796875, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=40.4761904761905, Blast_Score=174, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=37.1308016877637, Blast_Score=143, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=27.9527559055118, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=36.3128491620112, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=36.8715083798883, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=179, Percent_Identity=36.8715083798883, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=36.8715083798883, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=28, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=28.735632183908, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=208, Percent_Identity=29.3269230769231, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=197, Percent_Identity=26.3959390862944, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=278, Percent_Identity=25.8992805755396, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=278, Percent_Identity=25.8992805755396, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=23.8578680203046, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=42.0634920634921, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=42.0634920634921, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24653139, Length=275, Percent_Identity=36.3636363636364, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21357171, Length=227, Percent_Identity=29.9559471365639, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=32.1285140562249, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24650670, Length=198, Percent_Identity=31.8181818181818, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24654903, Length=181, Percent_Identity=27.6243093922652, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=22.4806201550388, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=36.7816091954023, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=36.7816091954023, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=36.7816091954023, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27020; Mature: 26889

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASEVLVSRDGAIATLTINRPRAKNSLNRAVFDGLRAQLVQLRDDDTVRAVIITGAEG
CCCHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
VFCAGADITAFDALRAEPLLGDRAAAGGHFWSELERFPKPVIAAVEKLALGGGTELALAC
EEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEE
DIAIAGESANFGVPEVKLGAIPGAGGTQRLIHAIGKSKAMALLLTGDFIDARKACDAGMV
EEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCE
AEVTADGQALPTALAMANRIAANSPLAVALAKDAALASFETPLAQGLEHEKRNFLVALRS
EEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCHHHHHHHHCCCEEEEEEEC
ADNLEGQAAFLGKRTPHFTGQ
CCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SEASEVLVSRDGAIATLTINRPRAKNSLNRAVFDGLRAQLVQLRDDDTVRAVIITGAEG
CCHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
VFCAGADITAFDALRAEPLLGDRAAAGGHFWSELERFPKPVIAAVEKLALGGGTELALAC
EEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEE
DIAIAGESANFGVPEVKLGAIPGAGGTQRLIHAIGKSKAMALLLTGDFIDARKACDAGMV
EEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCE
AEVTADGQALPTALAMANRIAANSPLAVALAKDAALASFETPLAQGLEHEKRNFLVALRS
EEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCHHHHHHHHCCCEEEEEEEC
ADNLEGQAAFLGKRTPHFTGQ
CCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]