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The map label for this gene is fdoI [H]

Identifier: 187929655

GI number: 187929655

Start: 2692414

End: 2693577

Strand: Reverse

Name: fdoI [H]

Synonym: Rpic_2583

Alternate gene names: 187929655

Gene position: 2693577-2692414 (Counterclockwise)

Preceding gene: 187929656

Following gene: 187929654

Centisome position: 68.32

GC content: 65.03

Gene sequence:

>1164_bases
ATGGGCACTTCCTGGAAACATCTTGTGATGGCCGTGCTGCTCGGCGGCGCGGCGGCGTGGGCGAGCGCGCAGCAGCCGGC
CAGTGCGCCGGCAGCCAACCATGCCCCGGTCAATCCGGCCGTACAAACGGCCCAGCCGTTCTCCAACATCGTCTCGGAGA
ACGTGTTTGATCTGCAGAAGCGCGATCTCGCCAAAGAGGCGCAGGACCAGGCCGCACGGCGCGCGACGCAACCGGGCAAC
AACGCGCCCGTCTGGCGCGAGGTGAACTCCGATCAGGCGCACTACGCCAGCATTCCCGGGCTGGAAGCCGGCGTGCTGAT
CCAGCGCACCGGGCAGAAATGGCGGCTGTTCCGCAATGGCGTGATCACCGTGTGGGGCGGTTGGCTCATGGTGCTGGTGC
CGTCGGCCATCCTGGCCTTCTTCCTTTGGCGCGGCATGATCCCGCTAAAAGAAGCGCCTACGGGCCGCAAGATCGAGCGC
TTCACGCCCACCGAGCGCTACGTGCACTGGACGATGGCGATCTCCTTCGTCACGCTCGGCGTGTCGGGCATCGTCATGCT
GTGGGGCAAGCATTTCCTGTTGCCGATCCTCGGTCATCAGTTGTTCGGCTGGCTGACGTATTTGCTGAAAAACCTGCACA
ACCTGGTGGGCCCGCTCTTCACGGTCAGCATCATCGTGGCCTTCGTGATTTGGGTGCGGGACAACCTGCCGCGCCAGGGC
GACGTGAAATGGCTGCTGAGCCTGGGGGGCATGTTTGCCGGAGAGCACGGCGCCGAAGTGCCGTCGCACCGCTTCAATGC
GGGCGAGAAACTCTGGTTCTGGGGCGGGCTCGTCGTGTTTGGGCTGATCCTGTCGGCTTCGGGCTGGACGCTCGATCGCA
TCGTCCCGGGCGTGGATTACTACCGCAGCACGATGCAACTGGCCGAGGTCATTCACGCGATCGCGGCGGTGGGCATGATG
AGCATGTCGCTGGGCCACATCTACATGGGCACGATCGGCATGGAAGGCGCTTACCGCGCCATGCGTGACGGTTATGTCGA
CGAGGCCTGGGCGAAGGAGCACCACGAACTTTGGTACGACGACGTCAAGGCCGGCAAGATTCCCGCGCAGCGGTCCCAGC
CGCCCGCGCCGCCGGCGCCGAGCGAGCAGCCCGCCAAGTCATGA

Upstream 100 bases:

>100_bases
CCGGCGACCGCACGAGCTGGGAGAACCAGATCCGCGCGCGCGGCCAATACCAGAACGAGTACAACAAGACGCCATGACGC
GCGCAAGCGGAGGTTCGTCC

Downstream 100 bases:

>100_bases
CGCCATCGCCTGCCACTCATTCGAGGATCCTCACCATGCATGCTTCCACGCTGACTCGCGCCGCAGGTATGGCACTTGCT
GCCGCCATGCTGGTCCTGAC

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGTSWKHLVMAVLLGGAAAWASAQQPASAPAANHAPVNPAVQTAQPFSNIVSENVFDLQKRDLAKEAQDQAARRATQPGN
NAPVWREVNSDQAHYASIPGLEAGVLIQRTGQKWRLFRNGVITVWGGWLMVLVPSAILAFFLWRGMIPLKEAPTGRKIER
FTPTERYVHWTMAISFVTLGVSGIVMLWGKHFLLPILGHQLFGWLTYLLKNLHNLVGPLFTVSIIVAFVIWVRDNLPRQG
DVKWLLSLGGMFAGEHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWTLDRIVPGVDYYRSTMQLAEVIHAIAAVGMM
SMSLGHIYMGTIGMEGAYRAMRDGYVDEAWAKEHHELWYDDVKAGKIPAQRSQPPAPPAPSEQPAKS

Sequences:

>Translated_387_residues
MGTSWKHLVMAVLLGGAAAWASAQQPASAPAANHAPVNPAVQTAQPFSNIVSENVFDLQKRDLAKEAQDQAARRATQPGN
NAPVWREVNSDQAHYASIPGLEAGVLIQRTGQKWRLFRNGVITVWGGWLMVLVPSAILAFFLWRGMIPLKEAPTGRKIER
FTPTERYVHWTMAISFVTLGVSGIVMLWGKHFLLPILGHQLFGWLTYLLKNLHNLVGPLFTVSIIVAFVIWVRDNLPRQG
DVKWLLSLGGMFAGEHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWTLDRIVPGVDYYRSTMQLAEVIHAIAAVGMM
SMSLGHIYMGTIGMEGAYRAMRDGYVDEAWAKEHHELWYDDVKAGKIPAQRSQPPAPPAPSEQPAKS
>Mature_386_residues
GTSWKHLVMAVLLGGAAAWASAQQPASAPAANHAPVNPAVQTAQPFSNIVSENVFDLQKRDLAKEAQDQAARRATQPGNN
APVWREVNSDQAHYASIPGLEAGVLIQRTGQKWRLFRNGVITVWGGWLMVLVPSAILAFFLWRGMIPLKEAPTGRKIERF
TPTERYVHWTMAISFVTLGVSGIVMLWGKHFLLPILGHQLFGWLTYLLKNLHNLVGPLFTVSIIVAFVIWVRDNLPRQGD
VKWLLSLGGMFAGEHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWTLDRIVPGVDYYRSTMQLAEVIHAIAAVGMMS
MSLGHIYMGTIGMEGAYRAMRDGYVDEAWAKEHHELWYDDVKAGKIPAQRSQPPAPPAPSEQPAKS

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=212, Percent_Identity=29.7169811320755, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787750, Length=225, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 42716; Mature: 42585

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTSWKHLVMAVLLGGAAAWASAQQPASAPAANHAPVNPAVQTAQPFSNIVSENVFDLQK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDLAKEAQDQAARRATQPGNNAPVWREVNSDQAHYASIPGLEAGVLIQRTGQKWRLFRNG
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCC
VITVWGGWLMVLVPSAILAFFLWRGMIPLKEAPTGRKIERFTPTERYVHWTMAISFVTLG
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
VSGIVMLWGKHFLLPILGHQLFGWLTYLLKNLHNLVGPLFTVSIIVAFVIWVRDNLPRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVKWLLSLGGMFAGEHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWTLDRIVPGVDY
CHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHCCCHHH
YRSTMQLAEVIHAIAAVGMMSMSLGHIYMGTIGMEGAYRAMRDGYVDEAWAKEHHELWYD
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
DVKAGKIPAQRSQPPAPPAPSEQPAKS
HHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTSWKHLVMAVLLGGAAAWASAQQPASAPAANHAPVNPAVQTAQPFSNIVSENVFDLQK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDLAKEAQDQAARRATQPGNNAPVWREVNSDQAHYASIPGLEAGVLIQRTGQKWRLFRNG
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCC
VITVWGGWLMVLVPSAILAFFLWRGMIPLKEAPTGRKIERFTPTERYVHWTMAISFVTLG
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
VSGIVMLWGKHFLLPILGHQLFGWLTYLLKNLHNLVGPLFTVSIIVAFVIWVRDNLPRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVKWLLSLGGMFAGEHGAEVPSHRFNAGEKLWFWGGLVVFGLILSASGWTLDRIVPGVDY
CHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHCCCHHH
YRSTMQLAEVIHAIAAVGMMSMSLGHIYMGTIGMEGAYRAMRDGYVDEAWAKEHHELWYD
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
DVKAGKIPAQRSQPPAPPAPSEQPAKS
HHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]