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The map label for this gene is nadE [H]

Identifier: 187929635

GI number: 187929635

Start: 2667589

End: 2669226

Strand: Reverse

Name: nadE [H]

Synonym: Rpic_2562

Alternate gene names: 187929635

Gene position: 2669226-2667589 (Counterclockwise)

Preceding gene: 187929637

Following gene: 187929634

Centisome position: 67.7

GC content: 64.84

Gene sequence:

>1638_bases
ATGACCGTCTCTGTCGCTCTCGCCCAGATCAACTGCACCGTCGGCGATTTCGCCGGCAATGCTGCCCGCATTGTCGCCGC
CGCGCGCGAAGCGCACCAGGCTGGTGCGCGCATCCTGCTCACGCCGGAACTGTCGCTCACGGGCTATCCGCCCGAAGACC
TGCTGCTGCGCCCGGCGTTCATCGACGCCAGCGCACGCGCGCTCGATGCGCTCATCGCCGAGCTGGCGGCATTCCCGGGC
TTGCACGTGCTGATCGGGCACCCACAACTCTCGCTGGAAGCCCCAGCGCCCGGCGTGCTGCCCAAGCCCACCAATAGCGC
CACAGTGGCCCTTGACGGCCGTGTGGTCGGCCGGTACGACAAGCTTGAACTGCCCAACAACGAGGTCTTTGACGAGAAGC
GCTACTTTCAGCCCGGCACCGAGCCCTTTGTGTTCGAAGTGGACGGCACGCGCTTCGGCGTCATCATCTGCGAAGACGCC
TGGTACGCCACGGCCACGGCCTATGCCAAGGCCGCCGGCGCGCAGGTTGTGTTGATCCCCAACGCCTCGCCGTACCACCT
CGACAAGGAAGACCTGCGCGAGCGGATCATCGGTGCCCGTGTGAAGGAATCGGGATTGCCGCATGTGTATGCAAACCTCG
TCGGCGGGCAGGATGAACTCGTGTTCGACGGGGGCTCGTTCGTGCTCGATGCCGAAGGCAACGTCGTCTTGCGGATGGGG
CAGTTTGTCGAGGGCGTGGGCTACGTGCAGTTCGACGGCGGACGCGCGCTGCCGGGCACGATCGTCCCGGAGCCTCCGCT
GGAAGCCCAGGTATACGAAGCGCTCAAGCTGGGCGTGCGCGACTACCTCGGCAAGAATGGCTTCCCGGGCGCAATCATCG
GCTTGTCGGGTGGCGTCGATTCAGCGTTGGTGCTGGCGATTGCCGTCGACGCGCTGGGCGCGGATCGCGTGCGCGCGGTC
ATGATGCCCTCGCGTTACACGGCAGATATCTCGTGGGTGGATGCGCGCGACATGGCGGCCCGGCTGGGCGTGCAGTACGA
CGAAATCGCCATCAGCCCGATGTTCGACGCATTCAAGGGTGCCTTGGCGGAGGAGTTCCGCGGGCTGCCCGAAGATGCGA
CGGAAGAGAACATCCAGGCGCGCATTCGCGGCACGCTGCTGATGGCACTGTCCAACAAGAGCGGCCGGATCGTGCTGACG
ACCGGCAACAAGAGTGAAATAGCGGTCGGCTACTGCACGCTGTACGGCGACATGGCCGGCGGTTTTGCCGTGATCAAGGA
TATCTTCAAGACGCTGGTCTACCGACTGTGCCGCTACCGCAATTCGTTGTCGGAGGTGATTCCGGAGCGCATCTTGACCC
GCGCACCATCGGCCGAGCTGCGCGAGAACCAGACCGACCAGGACAGCCTGCCCGAATACGATGCGCTCGACGCCATCGTG
CAGCGCTACATGGAGCAGAACCAGCCGGTGGCCGACATCATCGCGGCCGGGTTTGCCGAGGCCGACGTGCAGAAGATCGT
GCGCCTCATCAAGATCAATGAATACAAGCGGCGTCAGGCGCCGGTGGGCATCCGCGTGACGCCGCGGGCGTTCGGGCGCG
ACTGGCGGTATCCGATTACGTCGCGCTTCAAAGAGTAA

Upstream 100 bases:

>100_bases
ACCAGCGCGCGCTTGAGGCTCGGGAGCAAGGGGGCGAGAGGTCATGCCGCCGATTCTAATTCGAGCTTCGGATGCCGCGC
TTGCAATCCACATTGCCCCC

Downstream 100 bases:

>100_bases
GCCCCCGGCATGCTCGATTACTACGCCAAGCGCGCCCCTGAATATGAGCGCATCTATACGAAGCCCGAGCGGCAGGGCGA
TTTGGCGCGGTTGAAGGCGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTVSVALAQINCTVGDFAGNAARIVAAAREAHQAGARILLTPELSLTGYPPEDLLLRPAFIDASARALDALIAELAAFPG
LHVLIGHPQLSLEAPAPGVLPKPTNSATVALDGRVVGRYDKLELPNNEVFDEKRYFQPGTEPFVFEVDGTRFGVIICEDA
WYATATAYAKAAGAQVVLIPNASPYHLDKEDLRERIIGARVKESGLPHVYANLVGGQDELVFDGGSFVLDAEGNVVLRMG
QFVEGVGYVQFDGGRALPGTIVPEPPLEAQVYEALKLGVRDYLGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRAV
MMPSRYTADISWVDARDMAARLGVQYDEIAISPMFDAFKGALAEEFRGLPEDATEENIQARIRGTLLMALSNKSGRIVLT
TGNKSEIAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNSLSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIV
QRYMEQNQPVADIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTPRAFGRDWRYPITSRFKE

Sequences:

>Translated_545_residues
MTVSVALAQINCTVGDFAGNAARIVAAAREAHQAGARILLTPELSLTGYPPEDLLLRPAFIDASARALDALIAELAAFPG
LHVLIGHPQLSLEAPAPGVLPKPTNSATVALDGRVVGRYDKLELPNNEVFDEKRYFQPGTEPFVFEVDGTRFGVIICEDA
WYATATAYAKAAGAQVVLIPNASPYHLDKEDLRERIIGARVKESGLPHVYANLVGGQDELVFDGGSFVLDAEGNVVLRMG
QFVEGVGYVQFDGGRALPGTIVPEPPLEAQVYEALKLGVRDYLGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRAV
MMPSRYTADISWVDARDMAARLGVQYDEIAISPMFDAFKGALAEEFRGLPEDATEENIQARIRGTLLMALSNKSGRIVLT
TGNKSEIAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNSLSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIV
QRYMEQNQPVADIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTPRAFGRDWRYPITSRFKE
>Mature_544_residues
TVSVALAQINCTVGDFAGNAARIVAAAREAHQAGARILLTPELSLTGYPPEDLLLRPAFIDASARALDALIAELAAFPGL
HVLIGHPQLSLEAPAPGVLPKPTNSATVALDGRVVGRYDKLELPNNEVFDEKRYFQPGTEPFVFEVDGTRFGVIICEDAW
YATATAYAKAAGAQVVLIPNASPYHLDKEDLRERIIGARVKESGLPHVYANLVGGQDELVFDGGSFVLDAEGNVVLRMGQ
FVEGVGYVQFDGGRALPGTIVPEPPLEAQVYEALKLGVRDYLGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRAVM
MPSRYTADISWVDARDMAARLGVQYDEIAISPMFDAFKGALAEEFRGLPEDATEENIQARIRGTLLMALSNKSGRIVLTT
GNKSEIAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNSLSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIVQ
RYMEQNQPVADIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTPRAFGRDWRYPITSRFKE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=536, Percent_Identity=24.2537313432836, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59186; Mature: 59055

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSVALAQINCTVGDFAGNAARIVAAAREAHQAGARILLTPELSLTGYPPEDLLLRPAF
CEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHEEECHH
IDASARALDALIAELAAFPGLHVLIGHPQLSLEAPAPGVLPKPTNSATVALDGRVVGRYD
CCHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCEEECCCC
KLELPNNEVFDEKRYFQPGTEPFVFEVDGTRFGVIICEDAWYATATAYAKAAGAQVVLIP
CCCCCCCCCCCCHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEC
NASPYHLDKEDLRERIIGARVKESGLPHVYANLVGGQDELVFDGGSFVLDAEGNVVLRMG
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEECCCEEEECCCCEEEEEC
QFVEGVGYVQFDGGRALPGTIVPEPPLEAQVYEALKLGVRDYLGKNGFPGAIIGLSGGVD
HHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
SALVLAIAVDALGADRVRAVMMPSRYTADISWVDARDMAARLGVQYDEIAISPMFDAFKG
HHHHHHHHHHHCCCHHEEEEECCCCCCCCEEEECHHHHHHHHCCCCHHEEECHHHHHHHH
ALAEEFRGLPEDATEENIQARIRGTLLMALSNKSGRIVLTTGNKSEIAVGYCTLYGDMAG
HHHHHHCCCCCCCCHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCC
GFAVIKDIFKTLVYRLCRYRNSLSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHH
QRYMEQNQPVADIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTPRAFGRDWRYPIT
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCEEECHHHHCCCCCCCHH
SRFKE
HCCCC
>Mature Secondary Structure 
TVSVALAQINCTVGDFAGNAARIVAAAREAHQAGARILLTPELSLTGYPPEDLLLRPAF
EEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHEEECHH
IDASARALDALIAELAAFPGLHVLIGHPQLSLEAPAPGVLPKPTNSATVALDGRVVGRYD
CCHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCEEECCCC
KLELPNNEVFDEKRYFQPGTEPFVFEVDGTRFGVIICEDAWYATATAYAKAAGAQVVLIP
CCCCCCCCCCCCHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEC
NASPYHLDKEDLRERIIGARVKESGLPHVYANLVGGQDELVFDGGSFVLDAEGNVVLRMG
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEECCCEEEECCCCEEEEEC
QFVEGVGYVQFDGGRALPGTIVPEPPLEAQVYEALKLGVRDYLGKNGFPGAIIGLSGGVD
HHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
SALVLAIAVDALGADRVRAVMMPSRYTADISWVDARDMAARLGVQYDEIAISPMFDAFKG
HHHHHHHHHHHCCCHHEEEEECCCCCCCCEEEECHHHHHHHHCCCCHHEEECHHHHHHHH
ALAEEFRGLPEDATEENIQARIRGTLLMALSNKSGRIVLTTGNKSEIAVGYCTLYGDMAG
HHHHHHCCCCCCCCHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCC
GFAVIKDIFKTLVYRLCRYRNSLSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHH
QRYMEQNQPVADIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTPRAFGRDWRYPIT
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCEEECHHHHCCCCCCCHH
SRFKE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]