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The map label for this gene is pinE [H]

Identifier: 187929623

GI number: 187929623

Start: 2655890

End: 2656480

Strand: Reverse

Name: pinE [H]

Synonym: Rpic_2550

Alternate gene names: 187929623

Gene position: 2656480-2655890 (Counterclockwise)

Preceding gene: 187929624

Following gene: 187929611

Centisome position: 67.38

GC content: 54.99

Gene sequence:

>591_bases
ATGTTGTACGGATACGCCCGAGTCTCGACGCAAGAACAAGAGACTCACGCACAAACCGACGCACTGTTTCGAGCAGGCGT
CGGTTTTATTTTTTCCGAAAAAAAAAGCGGTGGAAGCACGGTAGGTAGACCAGAGCTAGAAAAGCTGCTCCGCTTGCTCA
AGCGTGGCGACACCGTCGTCGTCTACAAGCTAGACCGCATCGCGCGATCGCTAAAAGACCTGCTGCGCATCATCGAACGG
ATAGAAGAAAAGGGCGCACAGTTCCGATCGCTGACCGAGTCGCTCGACACCACAACACCAGCAGGCCGCATGCTATTTCA
CATGGTCGGCGCATTCGCAGAGTTTGAGCGAGAACTCATTCGCGAACGCACCAAAGCAGGCATGGCCGCAGCGGTAAAGC
GAGGCGTCAAACTCGGCAGGCACTACGCAATGAGCAGAGAAGACGAAGCCGAAGCGCTTCGCCTCTGGTACGAAGGCAAA
ATGACCCGAACAGCAATTGCACGCAAGTTCGGCGTCCATCTCAGCAGCATCAAGCGTGCAATTAAACGCCACCAAGCCAC
CATCCAACCAAGCCTACTAGACGCAGCATGA

Upstream 100 bases:

>100_bases
GGCGTACCGGCGACGGTACCGTGGGTTCGAATCCCACCCTCTCCGCCATACCGCTAGGGCAGTCGTACAATCCTCAGAAA
CAACACGTTTCAGGGGGTCT

Downstream 100 bases:

>100_bases
AACACGCCTTCATCGCTCTACTTGCTTGCATCACAATCCCCGCCGCAACCGCGCAAACCGTGAACACTTGCAAGATCAAC
GGCAAAACAGTTATCACCGA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MLYGYARVSTQEQETHAQTDALFRAGVGFIFSEKKSGGSTVGRPELEKLLRLLKRGDTVVVYKLDRIARSLKDLLRIIER
IEEKGAQFRSLTESLDTTTPAGRMLFHMVGAFAEFERELIRERTKAGMAAAVKRGVKLGRHYAMSREDEAEALRLWYEGK
MTRTAIARKFGVHLSSIKRAIKRHQATIQPSLLDAA

Sequences:

>Translated_196_residues
MLYGYARVSTQEQETHAQTDALFRAGVGFIFSEKKSGGSTVGRPELEKLLRLLKRGDTVVVYKLDRIARSLKDLLRIIER
IEEKGAQFRSLTESLDTTTPAGRMLFHMVGAFAEFERELIRERTKAGMAAAVKRGVKLGRHYAMSREDEAEALRLWYEGK
MTRTAIARKFGVHLSSIKRAIKRHQATIQPSLLDAA
>Mature_196_residues
MLYGYARVSTQEQETHAQTDALFRAGVGFIFSEKKSGGSTVGRPELEKLLRLLKRGDTVVVYKLDRIARSLKDLLRIIER
IEEKGAQFRSLTESLDTTTPAGRMLFHMVGAFAEFERELIRERTKAGMAAAVKRGVKLGRHYAMSREDEAEALRLWYEGK
MTRTAIARKFGVHLSSIKRAIKRHQATIQPSLLDAA

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=177, Percent_Identity=48.5875706214689, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1787827, Length=185, Percent_Identity=33.5135135135135, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787638, Length=185, Percent_Identity=33.5135135135135, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22153; Mature: 22153

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYGYARVSTQEQETHAQTDALFRAGVGFIFSEKKSGGSTVGRPELEKLLRLLKRGDTVV
CCCCCHHCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHHHCCCEEE
VYKLDRIARSLKDLLRIIERIEEKGAQFRSLTESLDTTTPAGRMLFHMVGAFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RERTKAGMAAAVKRGVKLGRHYAMSREDEAEALRLWYEGKMTRTAIARKFGVHLSSIKRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IKRHQATIQPSLLDAA
HHHHHHHCCCHHHCCC
>Mature Secondary Structure
MLYGYARVSTQEQETHAQTDALFRAGVGFIFSEKKSGGSTVGRPELEKLLRLLKRGDTVV
CCCCCHHCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHHHCCCEEE
VYKLDRIARSLKDLLRIIERIEEKGAQFRSLTESLDTTTPAGRMLFHMVGAFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RERTKAGMAAAVKRGVKLGRHYAMSREDEAEALRLWYEGKMTRTAIARKFGVHLSSIKRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IKRHQATIQPSLLDAA
HHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]