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The map label for this gene is narH [H]

Identifier: 187929594

GI number: 187929594

Start: 2630929

End: 2632455

Strand: Reverse

Name: narH [H]

Synonym: Rpic_2519

Alternate gene names: 187929594

Gene position: 2632455-2630929 (Counterclockwise)

Preceding gene: 187929595

Following gene: 187929593

Centisome position: 66.77

GC content: 61.3

Gene sequence:

>1527_bases
ATGAAAGTACGTGCACAGATTGCGATGGTGCTGAACCTGGACAAGTGCATCGGTTGCCATACCTGTTCGGTAACGTGCAA
GAACGTATGGACGAGCCGCGAAGGCATGGAATACGCGTGGTTCAACAACGTGGAGACCAAGCCCGGCATCGGTTATCCGA
AAGATTGGGAGAACCAGGAGCGGTGGCGCGGCGGCTGGGTACGCAAGGCTGACGGCAAGCTTCAGCCGAAGCTGGGCGGC
AAGTGGCGGCTGCTGGCGCAGATTTTTGCGAATCCGCATCTGCCCGAGATCGACGACTACTACGAGCCGTTCACGTTCGA
CTATGCCCACCTGCAAACCGCGGGCGATTCCGCCGCAATGCCGGTTGCACGGCCGCGCTCGCTCATCAGCGGCGAGCGCC
TGGAGAAGATCGAATGGGGCCCGAACTGGGAGGAAATCCTGGGCGGCGAGTTTGAGAAGCGGGCGAAGGACTACAACTTC
GAGAACGTACAGAAGGACATCTACGGCCAGTTCGAGAACACCTTCATGATGTACCTGCCGCGACTGTGCGAGCACTGCCT
GAACCCTGCCTGCGTGGCCGCATGCCCGTCGGGCTCGATCTACAAGCGCGAGGAAGACGGCATCGTGCTGGTCGACCAGG
ACAAGTGCCGGGGCTGGCGTATGTGCGTGTCGGGTTGCCCGTACAAGAAGATTTATTTCAACTGGAAGAGCGGCAAGGCG
GAGAAGTGCATCTTCTGCTATCCGCGCATCGAGGTTGGGCAGCCCACCGTGTGTTCGGAAACCTGCGTGGGCCGCATTCG
CTACCTGGGTGTGTTGCTGTACGACGCAGACCGCATTAGCGAGGCGGCAAGCGTGGAGCAAGAAACCGATCTGTATGAGT
CGCAGTTGTCGGTGTTTCTCGACCCGCACGATCCGCAGGTGATTGCACAGGCCGAGCGCGACGGCGTGCCCGCTGCATGG
ATCGAAGGCGCGCAGCGCTCGCCGGTGTACAAGATGGCGATCGACTGGAAGATCGCGTTTCCGCTGCATCCGGAATACCG
CACGCTGCCGATGGTGTGGTACGTGCCGCCACTCTCGCCGATCAATGCAGCCGCCAATAGCGGGGAGCTGGGCATGAACG
GCTATCTGCCCGATGTTGACTCGCTGCGCATTCCGTTGCGTTATCTCGCCAACCTGCTGACGGCGGGCAAGGAAGCGCCC
GTGAAGCTTGCACTCGAACGCATGCTGGCGATGCGTGCCTTCATGCGTGCGCGGCACGTCGATGGCGTGGCAGCGCCAGC
CGTGCTGGCACAGGCGGGGTTGACCACCGCGCAGGTCGAAGAGATGTACCGCTACCTGGCGATCGCCAACTATGAAGACC
GATTCGTCATCCCGACCACGCACCGCGAATATGCCGAAGATGCCTTCGACTTGCGCGCATCGTGTGGCTTTTCGTTTGGG
AACGGATGCTCGGACGGTACCTCAGACGTGGGGCTGTTCAGCTCGCGCAAGAGCGGGCGCACGATTCCGATCCGGCAGGA
GTCCTGA

Upstream 100 bases:

>100_bases
TTGGATCGAACCGCGATGAGTTCCTGATCGTGCGCAAGATGAAGCGGATCGACTGGCTTGACGACACCTCACCCGCCTCG
TTGCCTGCAGGAGCGCGCTC

Downstream 100 bases:

>100_bases
CATGATGGCAGCAACCACCGGATCGACGGGCGTTGTGTTTTCCGTGCTGTCGGCGCTGTTGGATTACCCCGACCCATCCA
CGCAGCGTGCCTTGCCGGAG

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQERWRGGWVRKADGKLQPKLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQTAGDSAAMPVARPRSLISGERLEKIEWGPNWEEILGGEFEKRAKDYNF
ENVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWKSGKA
EKCIFCYPRIEVGQPTVCSETCVGRIRYLGVLLYDADRISEAASVEQETDLYESQLSVFLDPHDPQVIAQAERDGVPAAW
IEGAQRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGELGMNGYLPDVDSLRIPLRYLANLLTAGKEAP
VKLALERMLAMRAFMRARHVDGVAAPAVLAQAGLTTAQVEEMYRYLAIANYEDRFVIPTTHREYAEDAFDLRASCGFSFG
NGCSDGTSDVGLFSSRKSGRTIPIRQES

Sequences:

>Translated_508_residues
MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQERWRGGWVRKADGKLQPKLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQTAGDSAAMPVARPRSLISGERLEKIEWGPNWEEILGGEFEKRAKDYNF
ENVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWKSGKA
EKCIFCYPRIEVGQPTVCSETCVGRIRYLGVLLYDADRISEAASVEQETDLYESQLSVFLDPHDPQVIAQAERDGVPAAW
IEGAQRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGELGMNGYLPDVDSLRIPLRYLANLLTAGKEAP
VKLALERMLAMRAFMRARHVDGVAAPAVLAQAGLTTAQVEEMYRYLAIANYEDRFVIPTTHREYAEDAFDLRASCGFSFG
NGCSDGTSDVGLFSSRKSGRTIPIRQES
>Mature_508_residues
MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQERWRGGWVRKADGKLQPKLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQTAGDSAAMPVARPRSLISGERLEKIEWGPNWEEILGGEFEKRAKDYNF
ENVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWKSGKA
EKCIFCYPRIEVGQPTVCSETCVGRIRYLGVLLYDADRISEAASVEQETDLYESQLSVFLDPHDPQVIAQAERDGVPAAW
IEGAQRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGELGMNGYLPDVDSLRIPLRYLANLLTAGKEAP
VKLALERMLAMRAFMRARHVDGVAAPAVLAQAGLTTAQVEEMYRYLAIANYEDRFVIPTTHREYAEDAFDLRASCGFSFG
NGCSDGTSDVGLFSSRKSGRTIPIRQES

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=73.0923694779116, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=72.0883534136546, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=127, Percent_Identity=39.3700787401575, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787122, Length=120, Percent_Identity=39.1666666666667, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=131, Percent_Identity=30.5343511450382, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=106, Percent_Identity=36.7924528301887, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57427; Mature: 57427

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQE
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
RWRGGWVRKADGKLQPKLGGKWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQTAGDSAAM
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCCCCEECHHHHCCCCCCCCC
PVARPRSLISGERLEKIEWGPNWEEILGGEFEKRAKDYNFENVQKDIYGQFENTFMMYLP
CCCCCHHHHCCCHHHCCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWKSGKA
HHHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIEVGQPTVCSETCVGRIRYLGVLLYDADRISEAASVEQETDLYESQLSVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHEEEE
DPHDPQVIAQAERDGVPAAWIEGAQRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCCEEEEHHCCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCH
INAAANSGELGMNGYLPDVDSLRIPLRYLANLLTAGKEAPVKLALERMLAMRAFMRARHV
HHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DGVAAPAVLAQAGLTTAQVEEMYRYLAIANYEDRFVIPTTHREYAEDAFDLRASCGFSFG
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC
NGCSDGTSDVGLFSSRKSGRTIPIRQES
CCCCCCCCCCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure
MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQE
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
RWRGGWVRKADGKLQPKLGGKWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQTAGDSAAM
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCCCCEECHHHHCCCCCCCCC
PVARPRSLISGERLEKIEWGPNWEEILGGEFEKRAKDYNFENVQKDIYGQFENTFMMYLP
CCCCCHHHHCCCHHHCCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWKSGKA
HHHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIEVGQPTVCSETCVGRIRYLGVLLYDADRISEAASVEQETDLYESQLSVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHEEEE
DPHDPQVIAQAERDGVPAAWIEGAQRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCCEEEEHHCCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCH
INAAANSGELGMNGYLPDVDSLRIPLRYLANLLTAGKEAPVKLALERMLAMRAFMRARHV
HHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DGVAAPAVLAQAGLTTAQVEEMYRYLAIANYEDRFVIPTTHREYAEDAFDLRASCGFSFG
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC
NGCSDGTSDVGLFSSRKSGRTIPIRQES
CCCCCCCCCCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]