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The map label for this gene is dadA2 [H]

Identifier: 187929546

GI number: 187929546

Start: 2576137

End: 2577405

Strand: Reverse

Name: dadA2 [H]

Synonym: Rpic_2467

Alternate gene names: 187929546

Gene position: 2577405-2576137 (Counterclockwise)

Preceding gene: 187929547

Following gene: 187929545

Centisome position: 65.37

GC content: 68.24

Gene sequence:

>1269_bases
ATGCAAATCGCAGTAGTCGGCGCCGGCATCATCGGCATCAGCACGGCCCATGCGCTGGCTCAGGAAGGCCATCAGGTCAC
CCTGGTCGAGCGCCATCCGGGCCCGGGCGAGGGCACCAGTTATGCCAACGGCGGCCAGTTGAGCTACAGCTATGTGGCGC
CGCTGGCCGGCCCCGGCGTGCTTTCGCATGTGCCGGGCTGGCTGTTGCGTCGCGATTCGCCGCTGCGGCTGAAGCCATCG
CTGGACCCGGCGTTGCTGCGCTGGGGGCTGCGCTTCATCGCGGCCTGCAATCGTGACCGTGCCGACCGCACCACGCGCGA
GCTGCTTGCGCTGTCGTTCTACAGCCGCGCACGCATGGAAGCGTTGCGGGAGGCGTCGCCGGATCTGGCGTTCAGCTTTG
CGCGCCGCGGCAAGCTGGTGGTGCATCGCGAGGCTGCGGCGTTTGAATCGGCCCGCGCGCAGGTCGGCTATCAGGCGACG
CTCGGCTGCGAGCAGCATGCATTGTCCGCCGACGAAACGGTGGCCCACGAGCCCGCGCTTGCCGGCGCGCGTGACCAGAT
CGTGGGCGCCATCTACACGCCCGACGAAGACGTGGCCGATTGTCACCAACTCTGCGTCGGCCTGTTCAACCGGTTGCGCG
CGATGCCAAACGTGACGCTGCGTTTCAATACCGGCGTAGATGCGTTGTGGACCGAAGGCCGCCGCGTGCGCGGTCTCCAT
CTGGCAGATGGCGAGCAGGTTGCCGCCGATGCGGTGGTGATGGCAGCGGGCGTGACGAGCGCCAGGCTGCTCAGCCCATT
GCGCATCGACCCGGGGCTGTATCCGCTCAAGGGTTACAGCATCAGCCTGCCGCTGGAGGAGGGCGATGTTGCCCCGTTGA
TCAGCGTGACGGATGCGGCGCGCAAGATCGTCTATGCCCGCATTGGGCAGACGCTGCGTGTGGCGGGCATGGCGGATCTG
GTGGGCTGGTCAACGGCGCTGGATACGCGCCGCGCCCAGACGTTGTATGACGAAACCCGCGCGCTGTTTCCGGGCGCCAT
GCGCGCCAATGACGCGGGTGCCGATGCGGCGCCGTGGGCTGGCATGCGGCCTGCAACGCCAACGGGCGTGCCCGTGGTGG
GGGCGTCGCCGGTGCAAGGGTTGTGGCTGAACGTGGGCCATGGTGCGCTGGGCTTTACGCTAGCGCTGGGCAGTGCGGGC
TTGCTGGCCGATCTGATTGCCGGCCGCAAGCCCGCGATTTCCCCAGTGCCCTATGCGCTGGCCGCGTGA

Upstream 100 bases:

>100_bases
GCCCATAGCTCCACAACATCCCTCCCAACAACTCCGCATTTGCCCAACCCCCGCCGGGCTCCTTAAGCTGCAACGACATT
CCAAAATACGCAGCTTCTTC

Downstream 100 bases:

>100_bases
CACAAGAACGAGAATCAAGAGACCGAGCGCGCAGGTCCGATGATGGGTATCCGGCCCGCGCCGATTGGTAACCAAAGTAG
TCTGGAGCGAACACACCCAT

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQIAVVGAGIIGISTAHALAQEGHQVTLVERHPGPGEGTSYANGGQLSYSYVAPLAGPGVLSHVPGWLLRRDSPLRLKPS
LDPALLRWGLRFIAACNRDRADRTTRELLALSFYSRARMEALREASPDLAFSFARRGKLVVHREAAAFESARAQVGYQAT
LGCEQHALSADETVAHEPALAGARDQIVGAIYTPDEDVADCHQLCVGLFNRLRAMPNVTLRFNTGVDALWTEGRRVRGLH
LADGEQVAADAVVMAAGVTSARLLSPLRIDPGLYPLKGYSISLPLEEGDVAPLISVTDAARKIVYARIGQTLRVAGMADL
VGWSTALDTRRAQTLYDETRALFPGAMRANDAGADAAPWAGMRPATPTGVPVVGASPVQGLWLNVGHGALGFTLALGSAG
LLADLIAGRKPAISPVPYALAA

Sequences:

>Translated_422_residues
MQIAVVGAGIIGISTAHALAQEGHQVTLVERHPGPGEGTSYANGGQLSYSYVAPLAGPGVLSHVPGWLLRRDSPLRLKPS
LDPALLRWGLRFIAACNRDRADRTTRELLALSFYSRARMEALREASPDLAFSFARRGKLVVHREAAAFESARAQVGYQAT
LGCEQHALSADETVAHEPALAGARDQIVGAIYTPDEDVADCHQLCVGLFNRLRAMPNVTLRFNTGVDALWTEGRRVRGLH
LADGEQVAADAVVMAAGVTSARLLSPLRIDPGLYPLKGYSISLPLEEGDVAPLISVTDAARKIVYARIGQTLRVAGMADL
VGWSTALDTRRAQTLYDETRALFPGAMRANDAGADAAPWAGMRPATPTGVPVVGASPVQGLWLNVGHGALGFTLALGSAG
LLADLIAGRKPAISPVPYALAA
>Mature_422_residues
MQIAVVGAGIIGISTAHALAQEGHQVTLVERHPGPGEGTSYANGGQLSYSYVAPLAGPGVLSHVPGWLLRRDSPLRLKPS
LDPALLRWGLRFIAACNRDRADRTTRELLALSFYSRARMEALREASPDLAFSFARRGKLVVHREAAAFESARAQVGYQAT
LGCEQHALSADETVAHEPALAGARDQIVGAIYTPDEDVADCHQLCVGLFNRLRAMPNVTLRFNTGVDALWTEGRRVRGLH
LADGEQVAADAVVMAAGVTSARLLSPLRIDPGLYPLKGYSISLPLEEGDVAPLISVTDAARKIVYARIGQTLRVAGMADL
VGWSTALDTRRAQTLYDETRALFPGAMRANDAGADAAPWAGMRPATPTGVPVVGASPVQGLWLNVGHGALGFTLALGSAG
LLADLIAGRKPAISPVPYALAA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=418, Percent_Identity=35.8851674641148, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 44577; Mature: 44577

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIAVVGAGIIGISTAHALAQEGHQVTLVERHPGPGEGTSYANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCH
LSHVPGWLLRRDSPLRLKPSLDPALLRWGLRFIAACNRDRADRTTRELLALSFYSRARME
HHHCCHHHEECCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ALREASPDLAFSFARRGKLVVHREAAAFESARAQVGYQATLGCEQHALSADETVAHEPAL
HHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHCCEEEECCCHHHCCCCCHHHCCCCCC
AGARDQIVGAIYTPDEDVADCHQLCVGLFNRLRAMPNVTLRFNTGVDALWTEGRRVRGLH
CCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEE
LADGEQVAADAVVMAAGVTSARLLSPLRIDPGLYPLKGYSISLPLEEGDVAPLISVTDAA
ECCCCHHHHHHHHHHCCCCHHHHCCCEEECCCCCEECCCEEEECCCCCCCCCEEHHHHHH
RKIVYARIGQTLRVAGMADLVGWSTALDTRRAQTLYDETRALFPGAMRANDAGADAAPWA
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GMRPATPTGVPVVGASPVQGLWLNVGHGALGFTLALGSAGLLADLIAGRKPAISPVPYAL
CCCCCCCCCCCEECCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHH
AA
CC
>Mature Secondary Structure
MQIAVVGAGIIGISTAHALAQEGHQVTLVERHPGPGEGTSYANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCH
LSHVPGWLLRRDSPLRLKPSLDPALLRWGLRFIAACNRDRADRTTRELLALSFYSRARME
HHHCCHHHEECCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ALREASPDLAFSFARRGKLVVHREAAAFESARAQVGYQATLGCEQHALSADETVAHEPAL
HHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHCCEEEECCCHHHCCCCCHHHCCCCCC
AGARDQIVGAIYTPDEDVADCHQLCVGLFNRLRAMPNVTLRFNTGVDALWTEGRRVRGLH
CCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEE
LADGEQVAADAVVMAAGVTSARLLSPLRIDPGLYPLKGYSISLPLEEGDVAPLISVTDAA
ECCCCHHHHHHHHHHCCCCHHHHCCCEEECCCCCEECCCEEEECCCCCCCCCEEHHHHHH
RKIVYARIGQTLRVAGMADLVGWSTALDTRRAQTLYDETRALFPGAMRANDAGADAAPWA
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GMRPATPTGVPVVGASPVQGLWLNVGHGALGFTLALGSAGLLADLIAGRKPAISPVPYAL
CCCCCCCCCCCEECCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHH
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]