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The map label for this gene is gcvA [H]

Identifier: 187929350

GI number: 187929350

Start: 2390582

End: 2391517

Strand: Reverse

Name: gcvA [H]

Synonym: Rpic_2271

Alternate gene names: 187929350

Gene position: 2391517-2390582 (Counterclockwise)

Preceding gene: 187929352

Following gene: 187929349

Centisome position: 60.66

GC content: 63.46

Gene sequence:

>936_bases
ATGCGCAAGCTGCCTCCTCTGAATGCCTTGCGCGCCTTTGAATCGGCGGCGCGCCATCTGAGTTTCACGCGTGCGGGCGA
CGAGCTGAACGTCACGCATGGCGCGATCAGCCGCCAGGTTCAGGCACTCGAGGCGTGGCTCGGCGTGTCGCTCTTTCGCA
GGCTGAATCGGCGGGTGGAACTGACTGATGCGGGTCAGCGCTACCTGTCGGAGATTGGCGTAGCACTTGACCGCATCAGC
ACGTCCAGCCAGGCGCTGCGCGACCGCGACGCGGCGCGCATCCTCCGCGTGAACAGCATCCCGACGTTCACCATGCGCTG
GCTGATTCCGCGGCTCTCCACGTTCCAGATGCGCTTTCCGCGCGTGGAGGTGCGGTTGACCACCTCCATCGAAGAGATCG
AGGACTTGCCCGACCATTTCGACGTGATCATTCGTGGCGGGCCGGAGATTCATGCGGGGTTTCGATCGGGACGCTTTCTG
GACGAGAGCCGCTTTCCGGTGTGCAGCCCTGTGTTGCTGGAGCGCTTGCCGTTGTCGACGCCGTCGGAGTTGCGGCATCA
CACGCTGCTGCATTCGGACACGCTGCCCTCCGTGTGGCCAAGCTGGCTGGTGCTGGCCGGCGTACCAAACCTGCGGCCTG
CGCATGCGCTCACGTTCGAGCATTTCTATCTGTCCATTCAAGCCGCCATCGATGGTTTGGGTGTCGCGATGGGGCCATCG
GCGCTTATCACGGATGATCTCGAGGAGGGGCGTCTTGTGGCACCGTTCCCCAATCTCGCGCTGGGCGCGCGCAGCTACTA
CTGGTACAGCCCTGCGCGCAATCTCGGTGACGAAGTGGTGCAGGCTTTTTGCGCGTGGCTCGGCGAGGACCGGCCACAAC
GTCACGCAAACGTGAACACATTTCCGATCCCCGGTAACGGAGGTAACGCCGCGTAA

Upstream 100 bases:

>100_bases
TGTAGTGTCGGCGAGAGCCAGAAGGCGCCAGCGTAGTCCCAGGAAGTGTGATCAACAATCGATATATACTGACCTGATCT
GTGCGAAAAAATCACGCGAT

Downstream 100 bases:

>100_bases
CACCCTAGAGGCGGAGGCGAAACGCGATGCTACGATGCCCCCAAGCTTGATTGCTTTGGAAAGGAGGTCCCCACATGAAG
AAGAACGCGTGGATTCTCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRKLPPLNALRAFESAARHLSFTRAGDELNVTHGAISRQVQALEAWLGVSLFRRLNRRVELTDAGQRYLSEIGVALDRIS
TSSQALRDRDAARILRVNSIPTFTMRWLIPRLSTFQMRFPRVEVRLTTSIEEIEDLPDHFDVIIRGGPEIHAGFRSGRFL
DESRFPVCSPVLLERLPLSTPSELRHHTLLHSDTLPSVWPSWLVLAGVPNLRPAHALTFEHFYLSIQAAIDGLGVAMGPS
ALITDDLEEGRLVAPFPNLALGARSYYWYSPARNLGDEVVQAFCAWLGEDRPQRHANVNTFPIPGNGGNAA

Sequences:

>Translated_311_residues
MRKLPPLNALRAFESAARHLSFTRAGDELNVTHGAISRQVQALEAWLGVSLFRRLNRRVELTDAGQRYLSEIGVALDRIS
TSSQALRDRDAARILRVNSIPTFTMRWLIPRLSTFQMRFPRVEVRLTTSIEEIEDLPDHFDVIIRGGPEIHAGFRSGRFL
DESRFPVCSPVLLERLPLSTPSELRHHTLLHSDTLPSVWPSWLVLAGVPNLRPAHALTFEHFYLSIQAAIDGLGVAMGPS
ALITDDLEEGRLVAPFPNLALGARSYYWYSPARNLGDEVVQAFCAWLGEDRPQRHANVNTFPIPGNGGNAA
>Mature_311_residues
MRKLPPLNALRAFESAARHLSFTRAGDELNVTHGAISRQVQALEAWLGVSLFRRLNRRVELTDAGQRYLSEIGVALDRIS
TSSQALRDRDAARILRVNSIPTFTMRWLIPRLSTFQMRFPRVEVRLTTSIEEIEDLPDHFDVIIRGGPEIHAGFRSGRFL
DESRFPVCSPVLLERLPLSTPSELRHHTLLHSDTLPSVWPSWLVLAGVPNLRPAHALTFEHFYLSIQAAIDGLGVAMGPS
ALITDDLEEGRLVAPFPNLALGARSYYWYSPARNLGDEVVQAFCAWLGEDRPQRHANVNTFPIPGNGGNAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=38.4083044982699, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=31.5068493150685, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=32.5174825174825, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=38.2978723404255, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787589, Length=273, Percent_Identity=27.1062271062271, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081978, Length=283, Percent_Identity=25.0883392226148, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=23.9344262295082, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34755; Mature: 34755

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLNALRAFESAARHLSFTRAGDELNVTHGAISRQVQALEAWLGVSLFRRLNRRVE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTDAGQRYLSEIGVALDRISTSSQALRDRDAARILRVNSIPTFTMRWLIPRLSTFQMRFP
ECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHEECC
RVEVRLTTSIEEIEDLPDHFDVIIRGGPEIHAGFRSGRFLDESRFPVCSPVLLERLPLST
EEEEEEECCHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCC
PSELRHHTLLHSDTLPSVWPSWLVLAGVPNLRPAHALTFEHFYLSIQAAIDGLGVAMGPS
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCCC
ALITDDLEEGRLVAPFPNLALGARSYYWYSPARNLGDEVVQAFCAWLGEDRPQRHANVNT
HHCCCCCCCCEEECCCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCE
FPIPGNGGNAA
ECCCCCCCCCC
>Mature Secondary Structure
MRKLPPLNALRAFESAARHLSFTRAGDELNVTHGAISRQVQALEAWLGVSLFRRLNRRVE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTDAGQRYLSEIGVALDRISTSSQALRDRDAARILRVNSIPTFTMRWLIPRLSTFQMRFP
ECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHEECC
RVEVRLTTSIEEIEDLPDHFDVIIRGGPEIHAGFRSGRFLDESRFPVCSPVLLERLPLST
EEEEEEECCHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCC
PSELRHHTLLHSDTLPSVWPSWLVLAGVPNLRPAHALTFEHFYLSIQAAIDGLGVAMGPS
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCCC
ALITDDLEEGRLVAPFPNLALGARSYYWYSPARNLGDEVVQAFCAWLGEDRPQRHANVNT
HHCCCCCCCCEEECCCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCE
FPIPGNGGNAA
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]