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The map label for this gene is nuoE2 [H]

Identifier: 187929289

GI number: 187929289

Start: 2321234

End: 2321737

Strand: Reverse

Name: nuoE2 [H]

Synonym: Rpic_2210

Alternate gene names: 187929289

Gene position: 2321737-2321234 (Counterclockwise)

Preceding gene: 187929290

Following gene: 187929288

Centisome position: 58.89

GC content: 62.3

Gene sequence:

>504_bases
ATGCTATCAGCAGAAGCTCTCAAGGAAATCGACCGGGCGGTCGCGAAATATCCCGCCGACCAGAAGCAGTCCGCCGTGAT
GGCGGCGCTGGCGGTCGCGCAGAGCGAGAAAGGCTGGGTCTCGCCCGAAGTCATGCAGTTCGTGGCCGAGTACCTCGAAA
TGCCGCCGGTGTGGGTCGAGGAAGTGGCGACGTTCTACAACATGTACGACACCAAGCCGGTGGGCCGCTTCAAGCTGTCG
GTGTGCACGAATCTGCCGTGCGCGCTGTCGGGCGGCGAGCGTGCTGCCGACTACCTGAAGAAGAAGCTGGGCATCGGTTT
CAACGAGACCACCGCCGACGGCACCTTCACGCTGAAGGAAGGCGAGTGCATGGGCGCGTGCGGTGATGCGCCGGTCATGA
TCGTCAACAACACCCACATGTGCAGCTTCATGAGCAACGAGAAGCTCGACGCGCTGATCGCCGACCTCCAGTCGAAGGCG
CCGACCAACGGAGCAGGCAAGTAA

Upstream 100 bases:

>100_bases
CGGTTTGACGCGCAGGCAAGCGCGGCTGTGAAACGAGGACGCACGCCGGGGTAAGGTAGTTAGGTAACACGCCCCGCGTC
CCGCACCAATCGCAACGAAC

Downstream 100 bases:

>100_bases
ATGACCTCTCTGCACGATCGACATATCCAGCCGTTGATTCTTGCCGGCCTGAACGGCGACAACTGGCACCTCGAAGATTA
CGTCAAGCGTGGCGGCTATC

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MLSAEALKEIDRAVAKYPADQKQSAVMAALAVAQSEKGWVSPEVMQFVAEYLEMPPVWVEEVATFYNMYDTKPVGRFKLS
VCTNLPCALSGGERAADYLKKKLGIGFNETTADGTFTLKEGECMGACGDAPVMIVNNTHMCSFMSNEKLDALIADLQSKA
PTNGAGK

Sequences:

>Translated_167_residues
MLSAEALKEIDRAVAKYPADQKQSAVMAALAVAQSEKGWVSPEVMQFVAEYLEMPPVWVEEVATFYNMYDTKPVGRFKLS
VCTNLPCALSGGERAADYLKKKLGIGFNETTADGTFTLKEGECMGACGDAPVMIVNNTHMCSFMSNEKLDALIADLQSKA
PTNGAGK
>Mature_167_residues
MLSAEALKEIDRAVAKYPADQKQSAVMAALAVAQSEKGWVSPEVMQFVAEYLEMPPVWVEEVATFYNMYDTKPVGRFKLS
VCTNLPCALSGGERAADYLKKKLGIGFNETTADGTFTLKEGECMGACGDAPVMIVNNTHMCSFMSNEKLDALIADLQSKA
PTNGAGK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=164, Percent_Identity=40.2439024390244, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1788621, Length=139, Percent_Identity=34.5323741007194, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=167, Percent_Identity=39.5209580838323, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859877, Length=168, Percent_Identity=37.5, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355353, Length=168, Percent_Identity=36.9047619047619, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18028; Mature: 18028

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAEALKEIDRAVAKYPADQKQSAVMAALAVAQSEKGWVSPEVMQFVAEYLEMPPVWVE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHH
EVATFYNMYDTKPVGRFKLSVCTNLPCALSGGERAADYLKKKLGIGFNETTADGTFTLKE
HHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
GECMGACGDAPVMIVNNTHMCSFMSNEKLDALIADLQSKAPTNGAGK
CCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSAEALKEIDRAVAKYPADQKQSAVMAALAVAQSEKGWVSPEVMQFVAEYLEMPPVWVE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHH
EVATFYNMYDTKPVGRFKLSVCTNLPCALSGGERAADYLKKKLGIGFNETTADGTFTLKE
HHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
GECMGACGDAPVMIVNNTHMCSFMSNEKLDALIADLQSKAPTNGAGK
CCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]