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The map label for this gene is nuoM [H]

Identifier: 187929281

GI number: 187929281

Start: 2311312

End: 2312778

Strand: Reverse

Name: nuoM [H]

Synonym: Rpic_2202

Alternate gene names: 187929281

Gene position: 2312778-2311312 (Counterclockwise)

Preceding gene: 187929282

Following gene: 187929280

Centisome position: 58.66

GC content: 58.83

Gene sequence:

>1467_bases
ATGGTTCTGTCTTTTGCGATCTGGCTGCCGATCTTCTTCGGCGTGCTGGTGCTGGCCTCGGGCTCAGACCGCAATCCCGG
TTATGTCCGCTGGATGTCGCTGATCGGCTCCGTAATCAGCTTCGTGATGACGCTCCCGCTCATCACGCGTTTCGACAAGA
CGACGGCTGCGATGCAGTTCGTTGAGAAATCGTCGTGGATCGAGCGCTTCCACATCAGCTACTACCTTGGAGTCGACGGC
ATTTCGATGTGGTTCGTGGTGCTGACCGCGTTCATCACCGTCATCGTCGTCATTGCCGCCTGGGAAGTCATCACCGAGCG
CGTGGCGCAATACATGGCCGCGTTCCTGATCCTGTCCGGTCTGATGATCGGCGTGTTTGCGTCGCTCGACGGTCTGCTGT
TCTACGTGTTCTTCGAAGCCACGCTGATCCCGATGTACATCATCATCGGTGTGTGGGGCGGCCCGAACCGCGTCTACGCG
GCATTCAAGTTCTTCCTGTACACGCTGCTTGGTTCGTTGCTGACGCTGGTTGCGCTGTTGTATCTGTATTTCCACAGCGG
CACGTTCGAAATCCTGCAGTGGCATCAGGTCAAGCTGTCGATGAACGAGCAGGTCCTGATCTTCCTCGCGTTTTTCATGG
CCTTTGCCGTGAAAGTGCCGATGTGGCCGGTGCACACCTGGCTGCCGGACGCCCACGTGGAAGCCCCGACCGGCGGCTCC
GTTGTGCTGGCTGCCATCATGCTGAAGCTGGGCGCCTACGGTTTCCTGCGGTTCTCCCTGCCGATCGCGCCGGACGCCAG
CCACAGCCTGTCCGCGTTCATCATCGCGATCTCGCTGGTTGCCGTGATCTACATCGGTCTGGTGGCGCTGGTGCAGGCCG
ACATGAAGAAGCTGGTCGCGTATTCGTCGATCGCGCACATGGGCTTCGTCACGCTGGGCTTCTTCATCTTCAACGAGATC
GGTGTGGAGGGCGGTATCGTCCAGATGATCTCGCACGGCTTCATCTCAGGCGCAATGTTCCTGTGTATTGGCGTGTTGTA
TGACCGCGTGCATTCGCGTCAGATTGCCGATTACGGCGGTGTGGTGAACACGATGCCGAAGTTTGCGGCGCTGTCGGTCT
TCTTTGCGATGGCCAACTGCGGCCTGCCGGCAACCTCCGGTTTCGTCGGCGAATTCATGGTGATCCTGGGTTCCGTCAAG
TTCAACTTCTGGATTGGCTTGTTGGCCGCCACGGCGCTGATCTTTGGCGCAGCGTATTCGCTGTGGATGGTCAAGCGCGT
GATCTTCGGTGATGTGACGCACAAGCATGTGGCCGAGCTGAAGGACCTGAATTGCCGCGAGTTCTTCATGCTGGGCGTGC
TCGCGATCGCCACCCTGTACATGGGCCTGTATCCGAAGCCCTTCACCGATGTCATGCATGCGTCCGTGGTCAATCTGCTG
ACCCACGTGGCTCAGTCGAAGCTGTAA

Upstream 100 bases:

>100_bases
GTCCGGCTTCATCTATGACTATGCGTTCGCGATGATCATCGGCACGGTTGGGCTGCTGACGTATTTCGTGTTTCTGGCAG
GCAAATAAGGGATTACAAAA

Downstream 100 bases:

>100_bases
GCAGCGAGAAGAAACTATGCAATCGTCCTCCTCTATTGCGGCTTTTGCCCCAATCGTTTTTCTCGCGCTGGGCATTGGTG
CCGTCAACTGGATTGACTTG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSVISFVMTLPLITRFDKTTAAMQFVEKSSWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVIAAWEVITERVAQYMAAFLILSGLMIGVFASLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYFHSGTFEILQWHQVKLSMNEQVLIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLSAFIIAISLVAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVTHKHVAELKDLNCREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQSKL

Sequences:

>Translated_488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSVISFVMTLPLITRFDKTTAAMQFVEKSSWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVIAAWEVITERVAQYMAAFLILSGLMIGVFASLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYFHSGTFEILQWHQVKLSMNEQVLIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLSAFIIAISLVAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVTHKHVAELKDLNCREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQSKL
>Mature_488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSVISFVMTLPLITRFDKTTAAMQFVEKSSWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVIAAWEVITERVAQYMAAFLILSGLMIGVFASLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYFHSGTFEILQWHQVKLSMNEQVLIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLSAFIIAISLVAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVTHKHVAELKDLNCREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQSKL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=349, Percent_Identity=36.3896848137536, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI251831117, Length=467, Percent_Identity=23.7687366167024, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1788613, Length=468, Percent_Identity=37.6068376068376, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=24.2957746478873, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788829, Length=469, Percent_Identity=27.5053304904051, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788831, Length=292, Percent_Identity=26.027397260274, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=358, Percent_Identity=23.463687150838, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788614, Length=349, Percent_Identity=24.9283667621776, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53944; Mature: 53944

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCC
>Mature Secondary Structure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HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]