BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is paaF [H]

Identifier: 187929248

GI number: 187929248

Start: 2282639

End: 2283418

Strand: Direct

Name: paaF [H]

Synonym: Rpic_2169

Alternate gene names: 187929248

Gene position: 2282639-2283418 (Clockwise)

Preceding gene: 187929247

Following gene: 187929249

Centisome position: 57.9

GC content: 64.49

Gene sequence:

>780_bases
ATGACCGCGCAACTGCTCTCCAAACGCATCGGTTCGACGCTGGTGCTGACGCTGTCCAACCCTGACGCGCGCAACGCGCT
GGACCCGGTGATGTACACCGCGTCGATGGAAGCGCTGGACCGCGCCGCCAACGACAACGAGATCCGCGCCATCATCTTCA
CGGGCGACGGCAACGCCTTCTGTGCGGGCGGTAATCTGAACCGCCTGCTGGAAAACCGTAGCAAGCCGCGCAGCGTTCAA
GAAGAAGGCATCGACGCGCTCAACCACTGGATCGAGACGATCCGCACGTTTCCCAAGCCGGTGATCGCCGCGGTGGAGGG
CCCAGCGGCCGGCGCAGGTTTCTCGCTGGTGCTGGCGTGCGATTTTGCGATTGCCGCGTCGGATGCGAAGTTCGTGATGG
CGTATGTGAACGTGGCGCTTACGCCGGACGGCGGCGGCTCATGGCACATCGCGCGCTTCCTGCCGCGCCCGCTGGCGAGC
GAAATCATCATGCTCGGCAAGCCTGTCAGCGCGGAGCGGCTGGCGCACTTCGGGCTCATCAATGAGGTGGTCAAACCTGG
CGAGGCGCTGGACCAGGCATTGGCACTCGCGGAAAAACTCGCTGCGCAATCGCCGCACGCGGTGGGCCGCATCAAGGGCC
TGATCGCCCACGCCGAAGACGCCTCGCTGCACGATCACCTCAAGGCCGAGCAGCACAGCTTTGTCGAAGCGCTGCACCAC
GCCGATGGCAACGAAGGCATCTCGGCCTTCCTGCAAAAGCGCAAACCCCAATACCGCTAG

Upstream 100 bases:

>100_bases
TCGCCCGAGCCCGTGGCTGCTGCGCCGTGCCCGTCTGGGCATGTCGCTGCTCACGCCTGATGCCTGACGCCTGATTGCCG
CTTACCCTGACGAGGCCGCC

Downstream 100 bases:

>100_bases
TTTCAGGCTCGCATGGTTCCCTTCCCGACACCCAACCGCCGCCGCGTCTACCTGATGCGTCACGGCGCCGTCACGTACTT
CGACGAAACCGGCCGGCCCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTAQLLSKRIGSTLVLTLSNPDARNALDPVMYTASMEALDRAANDNEIRAIIFTGDGNAFCAGGNLNRLLENRSKPRSVQ
EEGIDALNHWIETIRTFPKPVIAAVEGPAAGAGFSLVLACDFAIAASDAKFVMAYVNVALTPDGGGSWHIARFLPRPLAS
EIIMLGKPVSAERLAHFGLINEVVKPGEALDQALALAEKLAAQSPHAVGRIKGLIAHAEDASLHDHLKAEQHSFVEALHH
ADGNEGISAFLQKRKPQYR

Sequences:

>Translated_259_residues
MTAQLLSKRIGSTLVLTLSNPDARNALDPVMYTASMEALDRAANDNEIRAIIFTGDGNAFCAGGNLNRLLENRSKPRSVQ
EEGIDALNHWIETIRTFPKPVIAAVEGPAAGAGFSLVLACDFAIAASDAKFVMAYVNVALTPDGGGSWHIARFLPRPLAS
EIIMLGKPVSAERLAHFGLINEVVKPGEALDQALALAEKLAAQSPHAVGRIKGLIAHAEDASLHDHLKAEQHSFVEALHH
ADGNEGISAFLQKRKPQYR
>Mature_258_residues
TAQLLSKRIGSTLVLTLSNPDARNALDPVMYTASMEALDRAANDNEIRAIIFTGDGNAFCAGGNLNRLLENRSKPRSVQE
EGIDALNHWIETIRTFPKPVIAAVEGPAAGAGFSLVLACDFAIAASDAKFVMAYVNVALTPDGGGSWHIARFLPRPLASE
IIMLGKPVSAERLAHFGLINEVVKPGEALDQALALAEKLAAQSPHAVGRIKGLIAHAEDASLHDHLKAEQHSFVEALHHA
DGNEGISAFLQKRKPQYR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=29.3877551020408, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI221136756, Length=258, Percent_Identity=24.4186046511628, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI221136753, Length=258, Percent_Identity=24.4186046511628, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI116235487, Length=251, Percent_Identity=25.4980079681275, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI221307494, Length=258, Percent_Identity=24.4186046511628, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI25453481, Length=255, Percent_Identity=22.7450980392157, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538573, Length=255, Percent_Identity=22.7450980392157, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=25, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=25, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI70995211, Length=222, Percent_Identity=31.0810810810811, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI4757968, Length=214, Percent_Identity=26.1682242990654, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI49355787, Length=214, Percent_Identity=26.1682242990654, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=25.1028806584362, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=25.1028806584362, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=25.1028806584362, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=23.8888888888889, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=22.4215246636771, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=22.4215246636771, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=32.5757575757576, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=37.4517374517375, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=31.0606060606061, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI87082183, Length=241, Percent_Identity=29.8755186721992, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=30.0813008130081, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=27.6243093922652, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=25.9803921568627, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=31.1475409836066, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=30.7377049180328, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=27.8026905829596, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=26.4591439688716, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=25, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=27.0916334661355, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=23.4126984126984, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=231, Percent_Identity=23.8095238095238, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=231, Percent_Identity=24.6753246753247, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=222, Percent_Identity=24.7747747747748, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=235, Percent_Identity=23.4042553191489, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=238, Percent_Identity=23.109243697479, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=235, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=32.3770491803279, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=32.3770491803279, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=31.6, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=28.1632653061224, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654903, Length=195, Percent_Identity=29.2307692307692, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24667722, Length=180, Percent_Identity=23.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=23.8461538461538, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27753; Mature: 27622

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLLSKRIGSTLVLTLSNPDARNALDPVMYTASMEALDRAANDNEIRAIIFTGDGNAF
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
CAGGNLNRLLENRSKPRSVQEEGIDALNHWIETIRTFPKPVIAAVEGPAAGAGFSLVLAC
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCEEEEEE
DFAIAASDAKFVMAYVNVALTPDGGGSWHIARFLPRPLASEIIMLGKPVSAERLAHFGLI
HHHHCCCCHHHEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHH
NEVVKPGEALDQALALAEKLAAQSPHAVGRIKGLIAHAEDASLHDHLKAEQHSFVEALHH
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ADGNEGISAFLQKRKPQYR
CCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAQLLSKRIGSTLVLTLSNPDARNALDPVMYTASMEALDRAANDNEIRAIIFTGDGNAF
CHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
CAGGNLNRLLENRSKPRSVQEEGIDALNHWIETIRTFPKPVIAAVEGPAAGAGFSLVLAC
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCEEEEEE
DFAIAASDAKFVMAYVNVALTPDGGGSWHIARFLPRPLASEIIMLGKPVSAERLAHFGLI
HHHHCCCCHHHEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHH
NEVVKPGEALDQALALAEKLAAQSPHAVGRIKGLIAHAEDASLHDHLKAEQHSFVEALHH
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ADGNEGISAFLQKRKPQYR
CCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]