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The map label for this gene is gsiB [H]

Identifier: 187929240

GI number: 187929240

Start: 2274001

End: 2275599

Strand: Direct

Name: gsiB [H]

Synonym: Rpic_2161

Alternate gene names: 187929240

Gene position: 2274001-2275599 (Clockwise)

Preceding gene: 187929239

Following gene: 187929241

Centisome position: 57.68

GC content: 64.04

Gene sequence:

>1599_bases
ATGACAACCCGTCCCCTCTTGTGTGTTCCCATCCGCGCCCTGATCCGCAACCTCGCCACATGCGCGCTGGCTGCGGCTGC
CGCGATGCCGTTCGCCGCACAAGCCGACGACCTCCGCATCGGCCTGTCCGCCGACGTCACGTCGATGGACCCGCAATGGA
ACAACGCCGGCCCCAACAACGCCATGGCGCTGCATATCTTCGAGTCGCTGGTCTTCCTCGACAAGAACGGCCGCTACACG
CCCGGCCTGGCCACCTCGTGGAAGCCCGTCGACGCCAACACATGGGAGATCAAGCTGCGCCCCAATGTGAAATGGAGCGA
CGGCACGCCGTTCACCGGCGAAGACGTGAAGGCATCGATCGAGCGTCCGGCGCGCCTGACCAACAGCCCGGGCCCCTTCA
CCAGCTATACCAAGTCGATTACCGAAGTACAGATCGTCGATCCGCTGACGGTGCGGTTGAAGTTGTCGACGCCCAATTAC
GCGAGCATCCCCAACGATCTGAACAGCCTGCCCATCATTCCGAAGAAGGTGGCCAACGCGCCGCAGGCGGACTTTGACGC
GGGCCGCGCAATGATCGGCACGGGCCCGTTCCTGTTCGACCACTTTACGCGCGGGCAGGAAATCGTCCTGAAGAAGAACC
CCCACTACTGGGGCCGCGTACCGCAGTGGGACAAGGTGACCTTCAAGATCATCACCGACAATGCGGCTCGCACAGCCGCA
CTGCTCTCCGGCGATGTGGATGTCGTCGAGGCCGTACCGGCTGCGGACGTCCCGCGCATCAAGCAGGACCCCAAGTTCCA
CCTCGAACAGCAGGTGTCGTGGCGCACGATTTTCTGGCAGATGGATCAGAGCCGCGATGTGTCGCCGTTCGTGACCGACA
AGGCCGGCAAGCCGCTGGCCAAGAACCCGCTCAAGGACCATCGCGTGCGCGAGGCCATCGCGCTGGCCATCAACCGCGAC
GCCATCGTCAGCCGCGTGCTCGAAGGCCAGGGCGTGGTGGCCTCGAGCATCGTGTCACCGCAGATCTTCGGGCACCCGGG
CGGCAAGCCGATCGCATATGACCCAGCACGCGCCAAGAAACTGCTTGCCGAGGCCGGCTATCCGGACGGCTTCGGGCTCA
CGCTGCACGCCACCAACAACCGCTACCTGAATGACGCGCAAGTCGCGCAAACCACCGCGCAGCTGCTCACGCGCATCGGC
ATCCAGACGAAGGTTGAAACGTTGCCGGTGGCGGCGTATTTCTCGCGCGCGCGTCAGGGCGAGTTCTCGTTCATGATGCT
CGGCTGGGGCTCGGTGGCCGCCGATGTGGCGCTGCGCAGCATTCTCGGTACGCCCAACGCGCAAACCGGCTACGGCTCGT
GGAACTGGGGCCGCTACAGCAACCCGGAACTCGACAAACTGGTACAGACGTCGCTCTCAACGGTCTCGAGCGAACAGGCA
CACGAGCAGGCTGCCAAAACTGCGGCACGCTTTGCGCAGACCGACTACGCGATCATCCCGTCGCATCACCAACTAGCGAC
GTGGGCCATGCGCAAGGGCATCCGCTACGAGGCGCGCACTGACGAATGGTCGCTCGCGTGGTTGTTCACCAAGCAATGA

Upstream 100 bases:

>100_bases
TATTGATTGTCGGTTTGCGTCGCACGCGCCCAACCGGGCTGCGGCACAATGACGATCTTCCGCCACATGGCATCACCGCC
CGAACCCCAGGAGCCTCTCG

Downstream 100 bases:

>100_bases
TGATCGCTTGGTAACGAACACCGTGCCGTAACGCGCCGTTACGGCAAATTCGCCTGATGTGCCGCATTGGGGACGCATTG
GGGTAACTAGCAGGAACTTT

Product: family 5 extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTTRPLLCVPIRALIRNLATCALAAAAAMPFAAQADDLRIGLSADVTSMDPQWNNAGPNNAMALHIFESLVFLDKNGRYT
PGLATSWKPVDANTWEIKLRPNVKWSDGTPFTGEDVKASIERPARLTNSPGPFTSYTKSITEVQIVDPLTVRLKLSTPNY
ASIPNDLNSLPIIPKKVANAPQADFDAGRAMIGTGPFLFDHFTRGQEIVLKKNPHYWGRVPQWDKVTFKIITDNAARTAA
LLSGDVDVVEAVPAADVPRIKQDPKFHLEQQVSWRTIFWQMDQSRDVSPFVTDKAGKPLAKNPLKDHRVREAIALAINRD
AIVSRVLEGQGVVASSIVSPQIFGHPGGKPIAYDPARAKKLLAEAGYPDGFGLTLHATNNRYLNDAQVAQTTAQLLTRIG
IQTKVETLPVAAYFSRARQGEFSFMMLGWGSVAADVALRSILGTPNAQTGYGSWNWGRYSNPELDKLVQTSLSTVSSEQA
HEQAAKTAARFAQTDYAIIPSHHQLATWAMRKGIRYEARTDEWSLAWLFTKQ

Sequences:

>Translated_532_residues
MTTRPLLCVPIRALIRNLATCALAAAAAMPFAAQADDLRIGLSADVTSMDPQWNNAGPNNAMALHIFESLVFLDKNGRYT
PGLATSWKPVDANTWEIKLRPNVKWSDGTPFTGEDVKASIERPARLTNSPGPFTSYTKSITEVQIVDPLTVRLKLSTPNY
ASIPNDLNSLPIIPKKVANAPQADFDAGRAMIGTGPFLFDHFTRGQEIVLKKNPHYWGRVPQWDKVTFKIITDNAARTAA
LLSGDVDVVEAVPAADVPRIKQDPKFHLEQQVSWRTIFWQMDQSRDVSPFVTDKAGKPLAKNPLKDHRVREAIALAINRD
AIVSRVLEGQGVVASSIVSPQIFGHPGGKPIAYDPARAKKLLAEAGYPDGFGLTLHATNNRYLNDAQVAQTTAQLLTRIG
IQTKVETLPVAAYFSRARQGEFSFMMLGWGSVAADVALRSILGTPNAQTGYGSWNWGRYSNPELDKLVQTSLSTVSSEQA
HEQAAKTAARFAQTDYAIIPSHHQLATWAMRKGIRYEARTDEWSLAWLFTKQ
>Mature_531_residues
TTRPLLCVPIRALIRNLATCALAAAAAMPFAAQADDLRIGLSADVTSMDPQWNNAGPNNAMALHIFESLVFLDKNGRYTP
GLATSWKPVDANTWEIKLRPNVKWSDGTPFTGEDVKASIERPARLTNSPGPFTSYTKSITEVQIVDPLTVRLKLSTPNYA
SIPNDLNSLPIIPKKVANAPQADFDAGRAMIGTGPFLFDHFTRGQEIVLKKNPHYWGRVPQWDKVTFKIITDNAARTAAL
LSGDVDVVEAVPAADVPRIKQDPKFHLEQQVSWRTIFWQMDQSRDVSPFVTDKAGKPLAKNPLKDHRVREAIALAINRDA
IVSRVLEGQGVVASSIVSPQIFGHPGGKPIAYDPARAKKLLAEAGYPDGFGLTLHATNNRYLNDAQVAQTTAQLLTRIGI
QTKVETLPVAAYFSRARQGEFSFMMLGWGSVAADVALRSILGTPNAQTGYGSWNWGRYSNPELDKLVQTSLSTVSSEQAH
EQAAKTAARFAQTDYAIIPSHHQLATWAMRKGIRYEARTDEWSLAWLFTKQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=477, Percent_Identity=29.1404612159329, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789966, Length=445, Percent_Identity=28.7640449438202, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787762, Length=531, Percent_Identity=27.683615819209, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=24.3951612903226, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081878, Length=510, Percent_Identity=24.1176470588235, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=24.4935543278085, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789397, Length=535, Percent_Identity=24.1121495327103, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787551, Length=539, Percent_Identity=23.191094619666, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082063, Length=425, Percent_Identity=23.0588235294118, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58523; Mature: 58392

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRPLLCVPIRALIRNLATCALAAAAAMPFAAQADDLRIGLSADVTSMDPQWNNAGPNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
AMALHIFESLVFLDKNGRYTPGLATSWKPVDANTWEIKLRPNVKWSDGTPFTGEDVKASI
CEEHEEEHEEHEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHH
ERPARLTNSPGPFTSYTKSITEVQIVDPLTVRLKLSTPNYASIPNDLNSLPIIPKKVANA
HCCHHCCCCCCCCHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCC
PQADFDAGRAMIGTGPFLFDHFTRGQEIVLKKNPHYWGRVPQWDKVTFKIITDNAARTAA
CCCCCCCCCEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHH
LLSGDVDVVEAVPAADVPRIKQDPKFHLEQQVSWRTIFWQMDQSRDVSPFVTDKAGKPLA
HHCCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCEECCCCCCHH
KNPLKDHRVREAIALAINRDAIVSRVLEGQGVVASSIVSPQIFGHPGGKPIAYDPARAKK
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCEEECCCCCCCCEECHHHHHH
LLAEAGYPDGFGLTLHATNNRYLNDAQVAQTTAQLLTRIGIQTKVETLPVAAYFSRARQG
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCC
EFSFMMLGWGSVAADVALRSILGTPNAQTGYGSWNWGRYSNPELDKLVQTSLSTVSSEQA
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HEQAAKTAARFAQTDYAIIPSHHQLATWAMRKGIRYEARTDEWSLAWLFTKQ
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCEECCCCCEEEEEEEECC
>Mature Secondary Structure 
TTRPLLCVPIRALIRNLATCALAAAAAMPFAAQADDLRIGLSADVTSMDPQWNNAGPNN
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
AMALHIFESLVFLDKNGRYTPGLATSWKPVDANTWEIKLRPNVKWSDGTPFTGEDVKASI
CEEHEEEHEEHEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHH
ERPARLTNSPGPFTSYTKSITEVQIVDPLTVRLKLSTPNYASIPNDLNSLPIIPKKVANA
HCCHHCCCCCCCCHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCC
PQADFDAGRAMIGTGPFLFDHFTRGQEIVLKKNPHYWGRVPQWDKVTFKIITDNAARTAA
CCCCCCCCCEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHH
LLSGDVDVVEAVPAADVPRIKQDPKFHLEQQVSWRTIFWQMDQSRDVSPFVTDKAGKPLA
HHCCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCEECCCCCCHH
KNPLKDHRVREAIALAINRDAIVSRVLEGQGVVASSIVSPQIFGHPGGKPIAYDPARAKK
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCEEECCCCCCCCEECHHHHHH
LLAEAGYPDGFGLTLHATNNRYLNDAQVAQTTAQLLTRIGIQTKVETLPVAAYFSRARQG
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCC
EFSFMMLGWGSVAADVALRSILGTPNAQTGYGSWNWGRYSNPELDKLVQTSLSTVSSEQA
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HEQAAKTAARFAQTDYAIIPSHHQLATWAMRKGIRYEARTDEWSLAWLFTKQ
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA