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The map label for this gene is prpD [C]

Identifier: 187929236

GI number: 187929236

Start: 2268850

End: 2269248

Strand: Direct

Name: prpD [C]

Synonym: Rpic_2157

Alternate gene names: 187929236

Gene position: 2268850-2269248 (Clockwise)

Preceding gene: 187929235

Following gene: 187929237

Centisome position: 57.55

GC content: 64.41

Gene sequence:

>399_bases
ATGTCCGCACCGTTTGCGAACATCCGCACGAATCCGGACAAGGTCATCGTCGACGATGTGACCTTGCATGGATGCGCCGG
GCGCCTTGGGCCGGCCGTAACTGGAGCCGTCGAGCCGCATGGCACGATGGCTCCAGGCACCGCCCTCGCCGCCGCTCAGG
CAATCGAGGCGCACCAAGATACCGTCGCCCGCGGCCAACGCACCCATGCGCTGCGCATGAAAATTGCCCGCGTGGACGAC
AAGCGTTTCACCACGAATGACCACGACGCCAAGAAGCGTTCGATCGCCCATGGCATCACCGCCCGCATCAACGGCGGCAA
GGCGTTCGGCGGCGTCGTGGCGCAGTACCCGACCGGTCAGAAGCCATGTCGGTCAAGGCGTTTGCCGGTTTGTATGTGA

Upstream 100 bases:

>100_bases
CGGCACCGGCACCGGCACCGGCACCGGCACCGGCACCGGGCACGGCCCAACCGGCCCAGCAGTAACGCGCTGTCGCGCAA
GAGGCCCACAGGAACCGCCC

Downstream 100 bases:

>100_bases
TTTGATTTTGTCGATTTGATGTGTCCGCCAGGGGTGCCCGGCACCCCGTGCAATCCTCAAGCCTTGGAGTCATGCCATGC
CCCACAATCTGAAAAAAACA

Product: hypothetical protein

Products: cis-2-Methylaconitate; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSAPFANIRTNPDKVIVDDVTLHGCAGRLGPAVTGAVEPHGTMAPGTALAAAQAIEAHQDTVARGQRTHALRMKIARVDD
KRFTTNDHDAKKRSIAHGITARINGGKAFGGVVAQYPTGQKPCRSRRLPVCM

Sequences:

>Translated_132_residues
MSAPFANIRTNPDKVIVDDVTLHGCAGRLGPAVTGAVEPHGTMAPGTALAAAQAIEAHQDTVARGQRTHALRMKIARVDD
KRFTTNDHDAKKRSIAHGITARINGGKAFGGVVAQYPTGQKPCRSRRLPVCM
>Mature_131_residues
SAPFANIRTNPDKVIVDDVTLHGCAGRLGPAVTGAVEPHGTMAPGTALAAAQAIEAHQDTVARGQRTHALRMKIARVDDK
RFTTNDHDAKKRSIAHGITARINGGKAFGGVVAQYPTGQKPCRSRRLPVCM

Specific function: Catalyzes The Dehydration Of 2-Methylcitrate To 2- Methyl-Cis-Aconitate. Also Seems To Be Responsible For The Residual Aconitase Activity Of The Acnab-Null Strain. [C]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.79 [C]

Molecular weight: Translated: 13990; Mature: 13859

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPFANIRTNPDKVIVDDVTLHGCAGRLGPAVTGAVEPHGTMAPGTALAAAQAIEAHQD
CCCCCCCCCCCCCEEEEECEEECCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
TVARGQRTHALRMKIARVDDKRFTTNDHDAKKRSIAHGITARINGGKAFGGVVAQYPTGQ
HHHCCCCHHHHHHHEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCCC
KPCRSRRLPVCM
CCHHHCCCCCCC
>Mature Secondary Structure 
SAPFANIRTNPDKVIVDDVTLHGCAGRLGPAVTGAVEPHGTMAPGTALAAAQAIEAHQD
CCCCCCCCCCCCEEEEECEEECCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
TVARGQRTHALRMKIARVDDKRFTTNDHDAKKRSIAHGITARINGGKAFGGVVAQYPTGQ
HHHCCCCHHHHHHHEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCCC
KPCRSRRLPVCM
CCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Methylcitrate [C]

Specific reaction: 2-Methylcitrate --> cis-2-Methylaconitate + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA