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The map label for this gene is mdh [H]

Identifier: 187929232

GI number: 187929232

Start: 2265000

End: 2265986

Strand: Direct

Name: mdh [H]

Synonym: Rpic_2153

Alternate gene names: 187929232

Gene position: 2265000-2265986 (Clockwise)

Preceding gene: 187929208

Following gene: 187929233

Centisome position: 57.45

GC content: 64.03

Gene sequence:

>987_bases
ATGGCAAAAGCACCCATGCGCGTCGCAGTGACCGGCGCTGCTGGACAGATCGGTTACGCCCTACTGTTCCGCATCGCCGC
CGGCGAAATGCTGGGCAAGGATCAGCCCGTCATCCTGCAACTGCTGGAAATCCCCGATGAGAAGGCCCAGAAGGCGCTCA
AGGGTGTGATGATGGAGATCGAAGACTGCGCCTTCCCGCTGCTGGCTGGCATGCAAGCGCATTCCGACCCGATGACCGCG
TTCAAGGACGTCGACGTGGCCCTGCTGGTGGGCGCCCGCCCCCGCGGCCCGGGCATGGAGCGCGCGGACCTGCTGTCGGC
CAACGCCCAGATCTTCACGGCGCAAGGCAGGGCACTCAACGCCGTTGCCTCGCGCAACGTGAAGGTGCTGGTGGTCGGCA
ACCCGGCCAACACCAACGCCTACATCGCCATGAAGTCGGCGCCGGACCTGCCGCGCGAGAACTTCACCGCCATGCTGCGC
CTGGACCACAACCGCGCCCTGTCGCAGATCGCCGCCAAGACCGGCAAGCCCGTGTCGAGCATCGAAAAGCTGTTCGTGTG
GGGCAACCACAGCCCGACGATGTACGCCGACTACCGCTACGCCACCATCGACGGCAAGAGCGTCAAGGACATGATCAACG
ACCCGGTGTGGAACAACGACGTGTTCCTGCCGACCGTTGGCAAGCGCGGCGCCGCCATCATCGAAGCGCGCGGGCTGTCG
TCGGCGGCTTCGGCTGCCAATGCCGCCATCGACCACGTGCGTGACTGGGTGCTCGGTACCAACGGCAAGATCGTCACGAT
GGGCATCCCGTCGAACGGCGAATACGGCATTCCGAAGGACGTGATGTTCGGCTTCCCGGTCACCACCGAGAACGGCAAGT
ACAAGATCGTCGAAGGCCTGGAAATCGACGAGTACAGCCAAGGCAAGATCAAGATCACGCTCGACGAGCTGGAAGGCGAG
CGTGCAGGCGTGCAGCATCTGCTGTAA

Upstream 100 bases:

>100_bases
CGACCGTTGCCGGCAATGCCTGCGAAGCTGCACGGACATCCCGCCCGGATGCCCGCCGCACGCAGTGCGATCCGCTTTCA
CTACCGTTTGGAGAGTTCTC

Downstream 100 bases:

>100_bases
TCCGCATGATCTAAGAAAAAACCGGAGCGGATCGGCAGCGAACCCTCCGGTTTTTTTACACCCGCATTTCGAACAAGACA
CAGGCGTTGCTAGCGCCGAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMQAHSDPMTA
FKDVDVALLVGARPRGPGMERADLLSANAQIFTAQGRALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLR
LDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHVRDWVLGTNGKIVTMGIPSNGEYGIPKDVMFGFPVTTENGKYKIVEGLEIDEYSQGKIKITLDELEGE
RAGVQHLL

Sequences:

>Translated_328_residues
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMQAHSDPMTA
FKDVDVALLVGARPRGPGMERADLLSANAQIFTAQGRALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLR
LDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHVRDWVLGTNGKIVTMGIPSNGEYGIPKDVMFGFPVTTENGKYKIVEGLEIDEYSQGKIKITLDELEGE
RAGVQHLL
>Mature_327_residues
AKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMQAHSDPMTAF
KDVDVALLVGARPRGPGMERADLLSANAQIFTAQGRALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRL
DHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHVRDWVLGTNGKIVTMGIPSNGEYGIPKDVMFGFPVTTENGKYKIVEGLEIDEYSQGKIKITLDELEGER
AGVQHLL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=330, Percent_Identity=50, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI89886456, Length=323, Percent_Identity=25.3869969040248, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17561064, Length=324, Percent_Identity=50.9259259259259, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=50.763358778626, Blast_Score=247, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=47.9289940828402, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24583394, Length=322, Percent_Identity=54.0372670807453, Blast_Score=327, Evalue=7e-90,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35247; Mature: 35116

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEI
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHHHHHHHHH
EDCAFPLLAGMQAHSDPMTAFKDVDVALLVGARPRGPGMERADLLSANAQIFTAQGRALN
HHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCEEE
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRLDHNRALSQIAAKTGKPVSS
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHH
IEKLFVWGNHSPTMYADYRYATIDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
HEEEEEEECCCCCEEEEEEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCC
SAASAANAAIDHVRDWVLGTNGKIVTMGIPSNGEYGIPKDVMFGFPVTTENGKYKIVEGL
HHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCHHHEEECCEECCCCCEEEEECC
EIDEYSQGKIKITLDELEGERAGVQHLL
CCCCCCCCEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
AKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEI
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHHHHHHHHH
EDCAFPLLAGMQAHSDPMTAFKDVDVALLVGARPRGPGMERADLLSANAQIFTAQGRALN
HHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCEEE
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRLDHNRALSQIAAKTGKPVSS
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHH
IEKLFVWGNHSPTMYADYRYATIDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
HEEEEEEECCCCCEEEEEEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCC
SAASAANAAIDHVRDWVLGTNGKIVTMGIPSNGEYGIPKDVMFGFPVTTENGKYKIVEGL
HHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCHHHEEECCEECCCCCEEEEECC
EIDEYSQGKIKITLDELEGERAGVQHLL
CCCCCCCCEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]