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The map label for this gene is leuB [H]

Identifier: 187929222

GI number: 187929222

Start: 2255083

End: 2256153

Strand: Reverse

Name: leuB [H]

Synonym: Rpic_2143

Alternate gene names: 187929222

Gene position: 2256153-2255083 (Counterclockwise)

Preceding gene: 187929223

Following gene: 187929221

Centisome position: 57.23

GC content: 64.05

Gene sequence:

>1071_bases
ATGACCAAGATTGCAGTGTTGCCGGGTGACGGCATTGGTAAGGAAATCGTCGCCGAGGCCGTGAAGGTGCTCAAGGTGCT
GGGCGAGTCGTTCGAGATGGAATTCGCCCCGGTGGGCGGCGCGGGCTACGAGGCCAAGGGCCATCCGCTGCCGGAAGACA
CGCTCAAGCTCGCCAAGGAAGCTGACGCGATCCTGTTTGGCGCCGTGGGCGACTGGAAGTACGACACGCTGGCGCGCGAA
CTGCGCCCGGAGCAGGCCATTCTGGGCCTGCGCAAGCACCTGCAGCTGTTCGCCAATTTCCGTCCGGCGATCTGCTATCC
CGAGTTGACCGGCGCATCGAGCCTCAAGCCGGAAATCGTTGCCGGTCTGGATATCCTGATCGTGCGTGAGTTGAATGGCG
ACATTTACTTCGGCCAACCGCGCGGCGTGCGCGCGGCGCCCGACGGGCTGTTCGCCGGCGCGCGCGAAGGTTTCGACACC
ATGCGCTACGCCGAGCCGGAAATCCGCCGCATCGCGCACGTCGCCTTCCAGGCCGCCGCCAAGCGCGGCAAGAAGCTCTG
CAGCGTCGACAAGGCCAACGTGCTCGAGACGTTCCAGTTCTGGAAGGACATCGTCACCGACGTGCACAAGGAATATCCGG
AAGTCGAGTTGTCGCACATGTACGTGGACAACGCGGCCATGCAGCTCGTGAAGGCGCCGAAGAACTTCGACGTGATCGTC
ACCGGCAACATGTTCGGCGACATCCTCTCGGACGAGGCCGCCATGCTGACGGGCTCGATCGGCATGCTGCCGTCGGCGTC
GCTCGATGCGAATAACAAGGGCCTGTATGAGCCGTCGCACGGCTCCGCGCCGGACATCGCGGGCAAGGGCGTTGCCAACC
CGCTGGCGACGATCCTGTCGGCGGCGATGATGCTGCGCTATTCGCTGAACAAGGCCGAGCAGGCTGATCGCATCGAAACG
GCTGTCAAGAAGGTACTGGCGCAGGGCTACCGGACCGGCGACATCCTGACGCCGGGCTGCAAACAGGTGGGCACTGTGGA
GATGGGCGACGCGGTGGTCGCTGTCTTGTAA

Upstream 100 bases:

>100_bases
ACCAGATCAAGGCTTACGAAGCGCAGCGCCTGGCCAGGATGCCGTGGCTCGCCCACAAGCTCGTCGGCTGAGCGGACACC
AGAACAACAAGGATAACCAC

Downstream 100 bases:

>100_bases
CTCAAAACGCGCGTTGCGGTGCCGTCACATCCGGTGCAGCAATCGTCGTTTTTTCGGTGGACACATGCTGCAGTGCATTG
TGTGACCGCCAAATCGTGTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTKIAVLPGDGIGKEIVAEAVKVLKVLGESFEMEFAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWKYDTLARE
LRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPEIVAGLDILIVRELNGDIYFGQPRGVRAAPDGLFAGAREGFDT
MRYAEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDVIV
TGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIET
AVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAVL

Sequences:

>Translated_356_residues
MTKIAVLPGDGIGKEIVAEAVKVLKVLGESFEMEFAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWKYDTLARE
LRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPEIVAGLDILIVRELNGDIYFGQPRGVRAAPDGLFAGAREGFDT
MRYAEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDVIV
TGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIET
AVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAVL
>Mature_355_residues
TKIAVLPGDGIGKEIVAEAVKVLKVLGESFEMEFAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWKYDTLAREL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPEIVAGLDILIVRELNGDIYFGQPRGVRAAPDGLFAGAREGFDTM
RYAEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIETA
VKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAVL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.0030395136778, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=29.3929712460064, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=29.3929712460064, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4758582, Length=308, Percent_Identity=28.2467532467532, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI28178838, Length=308, Percent_Identity=28.8961038961039, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=35.4609929078014, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=52.112676056338, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788101, Length=377, Percent_Identity=33.9522546419098, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787381, Length=304, Percent_Identity=27.6315789473684, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=31.1953352769679, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=30.2670623145401, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.8591549295775, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17505779, Length=338, Percent_Identity=29.585798816568, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.3013698630137, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=35.7142857142857, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=31.8318318318318, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=342, Percent_Identity=28.6549707602339, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.258064516129, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=32.7485380116959, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=30.0291545189504, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=25.5192878338279, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=25.5192878338279, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=25.5192878338279, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=25.5192878338279, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078639, Length=335, Percent_Identity=25.6716417910448, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=28.7390029325513, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=28.7390029325513, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=30.8139534883721, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38317; Mature: 38185

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAVLPGDGIGKEIVAEAVKVLKVLGESFEMEFAPVGGAGYEAKGHPLPEDTLKLAKE
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHH
ADAILFGAVGDWKYDTLARELRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPEIV
CCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHH
AGLDILIVRELNGDIYFGQPRGVRAAPDGLFAGAREGFDTMRYAEPEIRRIAHVAFQAAA
HCCEEEEEEECCCCEEECCCCCCCCCCCCHHHCCHHCHHHHHCCCHHHHHHHHHHHHHHH
KRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDVIV
HHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCCCCEEEE
TGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILS
ECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AAMMLRYSLNKAEQADRIETAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEEC
>Mature Secondary Structure 
TKIAVLPGDGIGKEIVAEAVKVLKVLGESFEMEFAPVGGAGYEAKGHPLPEDTLKLAKE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHH
ADAILFGAVGDWKYDTLARELRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPEIV
CCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHH
AGLDILIVRELNGDIYFGQPRGVRAAPDGLFAGAREGFDTMRYAEPEIRRIAHVAFQAAA
HCCEEEEEEECCCCEEECCCCCCCCCCCCHHHCCHHCHHHHHCCCHHHHHHHHHHHHHHH
KRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDVIV
HHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCCCCEEEE
TGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILS
ECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AAMMLRYSLNKAEQADRIETAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]