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The map label for this gene is purF [H]

Identifier: 187929209

GI number: 187929209

Start: 2240390

End: 2241925

Strand: Reverse

Name: purF [H]

Synonym: Rpic_2130

Alternate gene names: 187929209

Gene position: 2241925-2240390 (Counterclockwise)

Preceding gene: 187929210

Following gene: 187929204

Centisome position: 56.86

GC content: 61.2

Gene sequence:

>1536_bases
ATGTGCGGTATCGTTGGGGTGGTCTCAGCCACGCCCGTCAATCAGCTGATCTACGACAGTCTGTTGCTCCTGCAACACCG
CGGGCAAGACGCTGCGGGCATTGCCACCGCATCAGGCAGCACGTTCCACATGCACAAGGCCAACGGCATGGTGCGTGACG
TGTTCCGCACGCGCAATATGCGCGGCCTGCCGGGTACGAGCGGTATCGGTCAGGTGCGCTATCCGACTGCAGGTTCCTCC
AGCGAAGAGGAGGCCCAACCGTTCTATGTCAACGCGCCGTTCGGCATCGTGTTGGCACACAACGGCAACCTGACGAACTC
GGATCAACTGCGCGACGAGATGTTCCGGCGCGATCGCCGCCACATCAACACGCAGTCTGATTCCGAAGTGCTGCTCAATG
TGCTGGCCGACGAGCTGCAACGCGCCAGCAACGACATTCCGCTGAATCGCGATTCCATCTTCACCGCGGTAGAAGGCCTG
CATCGCCGCGTGCGTGGTTCGTATGCCATCGCTGCCGAGATCTCGGGCTACGGCGTGCTGGCTGTGCGCGATCCGTTCGG
CATCCGCCCGCTCGCGCTGGGCACCCAGGAAACGCCGGATGGCGGCATCGAATATATGGTTGCTTCGGAGTCGGTGGCGC
TGGAAGGCATCGGCTTCAAGTTCGAGCGCGATGTGGCCCCCGGCGAAGCCATCTTCATTGACCTTGAGGGCAAGCTGCAC
ACCAAGCAATGCGCTGCGAACCCGGTACTTTCGCCATGCATTTTCGAATACGTGTACCTCGCCCGTCCGGACTCCCGCAT
GGATGGTGTGTCGGTGTACGACGCGCGACTGCGCATGGGCGACTACCTGGCTGAGAAGATCAAGCGCGAGGTGACCGACA
GGATCGACGTGGTGATGCCGATTCCGGATTCGTCCCGCCCAGCTGCCATGCAGGTGGCTAACCACCTGGGCGTGCCGTAT
CGCGAAGGTTTCTTCAAGAACCGATACGTGGGCCGTACCTTCATCATGCCGGGTCAGGCGGTACGCAAGAAATCTGTGCG
CCAGAAGCTCAACGCCATGGCAATCGAGTTCAAGGACAAGAACGTGCTGATCGTCGATGACTCCATCGTGCGCGGCACGA
CGTCGTCGGAGATCGTGCAGATGGCACGCGATGCCGGTGCCAAGAAGGTGATCTTCGCTTCGGCCGCGCCGCCGGTGAAG
TTCCCCAACGTGTACGGTATCGACATGCCGACGCGCGGCGAACTGGTGGCTTATGGCCGCACGCACGAGGAGATTGCCAA
GATCATCGGCGCAGACCAGCTTGTGTACCAGGATGTGGAGGACATGAAGCGCGCGGTGCGCGATGTCAATCCGGCACTGA
AGGATTTCGATGCGTCATGCTTTGACGGCAAGTACGTGACGGGCGACATTGACGAGGCGTACCTCGATCGCCTGGAGGCC
GCGCGTAACAATTCGGCCAAGGCGGATCGTGAGAACGCGCAGAACGATGATCCGCGCACGCAACTGCATCTGCAGCGCTC
CGCCGTGAACGAGTAA

Upstream 100 bases:

>100_bases
GATCTGGAGCGCCTGGCCACAAGCTAGGCGATCCGGGAAGCTTTTTCGGCGCCCGCAACGGGTGTGGGCGCCACCAAATA
CGTTCTCGCTGGAGTTCGAC

Downstream 100 bases:

>100_bases
TTCTCGAGCGCCGTAAAAGAAAGCCCGTCATCGCGACGGGCTTTTTTGTTGGACGTCGGGATCGCGCTACTTGAGCATGC
GACGGCGCAGCAGCCAGAGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATASGSTFHMHKANGMVRDVFRTRNMRGLPGTSGIGQVRYPTAGSS
SEEEAQPFYVNAPFGIVLAHNGNLTNSDQLRDEMFRRDRRHINTQSDSEVLLNVLADELQRASNDIPLNRDSIFTAVEGL
HRRVRGSYAIAAEISGYGVLAVRDPFGIRPLALGTQETPDGGIEYMVASESVALEGIGFKFERDVAPGEAIFIDLEGKLH
TKQCAANPVLSPCIFEYVYLARPDSRMDGVSVYDARLRMGDYLAEKIKREVTDRIDVVMPIPDSSRPAAMQVANHLGVPY
REGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKDKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVK
FPNVYGIDMPTRGELVAYGRTHEEIAKIIGADQLVYQDVEDMKRAVRDVNPALKDFDASCFDGKYVTGDIDEAYLDRLEA
ARNNSAKADRENAQNDDPRTQLHLQRSAVNE

Sequences:

>Translated_511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATASGSTFHMHKANGMVRDVFRTRNMRGLPGTSGIGQVRYPTAGSS
SEEEAQPFYVNAPFGIVLAHNGNLTNSDQLRDEMFRRDRRHINTQSDSEVLLNVLADELQRASNDIPLNRDSIFTAVEGL
HRRVRGSYAIAAEISGYGVLAVRDPFGIRPLALGTQETPDGGIEYMVASESVALEGIGFKFERDVAPGEAIFIDLEGKLH
TKQCAANPVLSPCIFEYVYLARPDSRMDGVSVYDARLRMGDYLAEKIKREVTDRIDVVMPIPDSSRPAAMQVANHLGVPY
REGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKDKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVK
FPNVYGIDMPTRGELVAYGRTHEEIAKIIGADQLVYQDVEDMKRAVRDVNPALKDFDASCFDGKYVTGDIDEAYLDRLEA
ARNNSAKADRENAQNDDPRTQLHLQRSAVNE
>Mature_511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATASGSTFHMHKANGMVRDVFRTRNMRGLPGTSGIGQVRYPTAGSS
SEEEAQPFYVNAPFGIVLAHNGNLTNSDQLRDEMFRRDRRHINTQSDSEVLLNVLADELQRASNDIPLNRDSIFTAVEGL
HRRVRGSYAIAAEISGYGVLAVRDPFGIRPLALGTQETPDGGIEYMVASESVALEGIGFKFERDVAPGEAIFIDLEGKLH
TKQCAANPVLSPCIFEYVYLARPDSRMDGVSVYDARLRMGDYLAEKIKREVTDRIDVVMPIPDSSRPAAMQVANHLGVPY
REGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKDKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVK
FPNVYGIDMPTRGELVAYGRTHEEIAKIIGADQLVYQDVEDMKRAVRDVNPALKDFDASCFDGKYVTGDIDEAYLDRLEA
ARNNSAKADRENAQNDDPRTQLHLQRSAVNE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.6315789473684, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=60, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1790167, Length=234, Percent_Identity=28.6324786324786, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=488, Percent_Identity=35.4508196721311, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=50.6958250497018, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=36.5461847389558, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=37.4476987447699, Blast_Score=275, Evalue=7e-74,
Organism=Drosophila melanogaster, GI28573187, Length=508, Percent_Identity=35.2362204724409, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56360; Mature: 56360

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATASGSTFHMHKANGMVRDVFRTRNM
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTSGIGQVRYPTAGSSSEEEAQPFYVNAPFGIVLAHNGNLTNSDQLRDEMFRRDRR
CCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHH
HINTQSDSEVLLNVLADELQRASNDIPLNRDSIFTAVEGLHRRVRGSYAIAAEISGYGVL
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
AVRDPFGIRPLALGTQETPDGGIEYMVASESVALEGIGFKFERDVAPGEAIFIDLEGKLH
EEECCCCCCEEECCCCCCCCCCEEEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCEE
TKQCAANPVLSPCIFEYVYLARPDSRMDGVSVYDARLRMGDYLAEKIKREVTDRIDVVMP
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IPDSSRPAAMQVANHLGVPYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKDK
CCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEEECC
NVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRGELVAYGR
EEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEEECC
THEEIAKIIGADQLVYQDVEDMKRAVRDVNPALKDFDASCFDGKYVTGDIDEAYLDRLEA
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
ARNNSAKADRENAQNDDPRTQLHLQRSAVNE
HCCCCCCCCHHCCCCCCCHHHEEEHHHHCCC
>Mature Secondary Structure
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATASGSTFHMHKANGMVRDVFRTRNM
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTSGIGQVRYPTAGSSSEEEAQPFYVNAPFGIVLAHNGNLTNSDQLRDEMFRRDRR
CCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHH
HINTQSDSEVLLNVLADELQRASNDIPLNRDSIFTAVEGLHRRVRGSYAIAAEISGYGVL
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
AVRDPFGIRPLALGTQETPDGGIEYMVASESVALEGIGFKFERDVAPGEAIFIDLEGKLH
EEECCCCCCEEECCCCCCCCCCEEEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCEE
TKQCAANPVLSPCIFEYVYLARPDSRMDGVSVYDARLRMGDYLAEKIKREVTDRIDVVMP
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IPDSSRPAAMQVANHLGVPYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKDK
CCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEEECC
NVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRGELVAYGR
EEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEEECC
THEEIAKIIGADQLVYQDVEDMKRAVRDVNPALKDFDASCFDGKYVTGDIDEAYLDRLEA
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
ARNNSAKADRENAQNDDPRTQLHLQRSAVNE
HCCCCCCCCHHCCCCCCCHHHEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]