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The map label for this gene is phoB [H]

Identifier: 187929100

GI number: 187929100

Start: 2127927

End: 2128640

Strand: Reverse

Name: phoB [H]

Synonym: Rpic_2021

Alternate gene names: 187929100

Gene position: 2128640-2127927 (Counterclockwise)

Preceding gene: 187929101

Following gene: 187929099

Centisome position: 53.99

GC content: 65.13

Gene sequence:

>714_bases
ATGCCGAGCAGTATTCTCGTCGTAGAAGACGAACCGGCGATCGCCGAGCTGATCGCCGTGAACCTGCAGCACGCAGGGCA
CTACCCGATCCGCGCGTATAACGCCGAGCAGGCGCTGTCGCTGATGAGCGACGTGTTGCCCGACCTCGTCCTGCTCGATT
GGATGCTGCCGGGCAAATCGGGCGTGATGTTCGCCAAGGAGCTGCGTGCCAACGAGCGCACGCGGCAGATCCCGATCATC
ATGCTGACGGCCCGCAGCGAAGAGCAGGACAAGATCATGGGTCTGGACAGCGGCGCGGACGATTACGTCACCAAGCCGTT
CTCGCCCAAGGAACTGCTCGCACGCATCAAAGCGGTGCTGCGCCGCCGCGCGCCGCAGCTGACCGACGACGTGGTGGCGA
TCAACGGCTTGAAGCTGGACCCGGCCACGCACCGCGTGACGGCCACGAACGTCGAGGCGGAAAACCCGATCAAGCTGGAC
CTCGGACCGACGGAGTTCCGCCTGCTGCATTTCCTGATGACGCATCCGGAGCGCGTGCACAGCCGCTCGCAGTTGCTCGA
CCAGGTGTGGGGCGACCACGTCTTCGTGGAAGAGCGCACGGTGGACGTGCACATCAAGCGCCTTCGCGCAGCGCTCGCTC
CGGGCGGCTACAGCAACATGATCGAAACCGTGCGCGGCAGCGGCTATCGCCTCGCGCGCAATCCGGGGCAGTAA

Upstream 100 bases:

>100_bases
CATCGTCAAGGGAACCGACGTCCGGCATGCCGGGCGCGAGGCGCTGGAGCGCGAGATCCTGGGCGAAGGCGAATAAGAAG
GCAGGACTGGAGACGTGAGC

Downstream 100 bases:

>100_bases
CGCAATAACGCATCCGTCAGGCGAACGCGCGAGCCGGTTCTTTTTCCATCGCGCGTTCACACGCCCGTCGCGGTCCAGCG
CATAAAATGCTTTCATGAAC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGVMFAKELRANERTRQIPII
MLTARSEEQDKIMGLDSGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLKLDPATHRVTATNVEAENPIKLD
LGPTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALAPGGYSNMIETVRGSGYRLARNPGQ

Sequences:

>Translated_237_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGVMFAKELRANERTRQIPII
MLTARSEEQDKIMGLDSGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLKLDPATHRVTATNVEAENPIKLD
LGPTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALAPGGYSNMIETVRGSGYRLARNPGQ
>Mature_236_residues
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGVMFAKELRANERTRQIPIIM
LTARSEEQDKIMGLDSGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLKLDPATHRVTATNVEAENPIKLDL
GPTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALAPGGYSNMIETVRGSGYRLARNPGQ

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=232, Percent_Identity=56.4655172413793, Blast_Score=280, Evalue=4e-77,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=40.7894736842105, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=38.1974248927039, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=38.1974248927039, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=35.5555555555556, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786784, Length=234, Percent_Identity=33.7606837606838, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786911, Length=232, Percent_Identity=36.2068965517241, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=32.4894514767932, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI2367329, Length=235, Percent_Identity=36.5957446808511, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787229, Length=239, Percent_Identity=33.8912133891213, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789402, Length=228, Percent_Identity=33.7719298245614, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790552, Length=231, Percent_Identity=33.7662337662338, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI145693140, Length=242, Percent_Identity=30.9917355371901, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=37.962962962963, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26496; Mature: 26365

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GVMFAKELRANERTRQIPIIMLTARSEEQDKIMGLDSGADDYVTKPFSPKELLARIKAVL
CCHHHHHHHCCCHHCCCCEEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLKLDPATHRVTATNVEAENPIKLDLGPTEFRLLHFLMTHPERVH
HHCCCCCCCCEEEECCEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHCHHHHH
SRSQLLDQVWGDHVFVEERTVDVHIKRLRAALAPGGYSNMIETVRGSGYRLARNPGQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GVMFAKELRANERTRQIPIIMLTARSEEQDKIMGLDSGADDYVTKPFSPKELLARIKAVL
CCHHHHHHHCCCHHCCCCEEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLKLDPATHRVTATNVEAENPIKLDLGPTEFRLLHFLMTHPERVH
HHCCCCCCCCEEEECCEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHCHHHHH
SRSQLLDQVWGDHVFVEERTVDVHIKRLRAALAPGGYSNMIETVRGSGYRLARNPGQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]