Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 187929053

GI number: 187929053

Start: 2082603

End: 2083637

Strand: Reverse

Name: pheS

Synonym: Rpic_1973

Alternate gene names: 187929053

Gene position: 2083637-2082603 (Counterclockwise)

Preceding gene: 187929054

Following gene: 187929052

Centisome position: 52.85

GC content: 61.45

Gene sequence:

>1035_bases
ATGTCACTGGATCTGGATCAAGTCGTCGTCGATGCCCAAAATGCGTTTGCCTCGGTGGAGGACAACGCCTCGCTGGAAAA
CGAGAAGGCACGCTTTCTCGGTAAGTCCGGCGTGCTGACCGACTTGCTCAAAGGCTTGGGCAAGCTCGATCCGGAGACAC
GCAAGACCGAAGGCGCGCGTATCAACCAAGCCAAGAGCCGCGTTGAAGAGGCATTGACGGCCCGCCGCCAGGCGTTGGCC
GACGCGCTGATGAACGCACGCCTCGCAGCCGAGGCGATCGACGTAACGCTGCCCGGCCGCGACGTGGCCGAGGGCAGCTT
GCACCCGGTGATGAACACCTGGGAGCGCGTCGCCAAGATCTTCGGCTCGATCGGTTTCGATGTGGCTGACGGCCCCGAAA
TCGAATCCGACTGGATGAACTTCACGGCGCTGAACAACCCGGACAACCATCCGGCGCGCTCGATGCAGGACACGTTCTAC
ATCGACGGTCGCGACAGCGAAGACAAGCTCCTGCTGCTGCGCACGCACACCAGCCCGATGCAGGTGCGCTATGCCCGCAT
GCACGTCGAGAAGTACAAGCATCTCGACCGTATCCCCCCCATCAAGGTCATCGCGCCGGGCCGCACATACCGTGTCGACA
GCGATGCTACGCACTCGCCGATGTTCCATCAGGTCGAAGGCCTCTGGATTGCCGACAACATCAGCTTTGCCGATCTGAAG
GGCGTGTACACCGATTTCCTGCGGAACTTCTTCGAGCGCGACGACATCCAGGTGCGTTTCCGCCCATCGTATTTCCCGTT
CACCGAGCCGTCGGCCGAGATCGACATGGCGTTCGGCAACGGCAAATGGCTGGAGATTTCCGGTTCCGGCCAGGTGCATC
CGACCGTGGTGCGCAACATGGGCCTGGACCCTGAGCGCTACATCGGCTTTGCATTCGGCTCCGGCCTGGAGCGTCTGACG
ATGCTGCGCTATGGCATCAACGATCTGCGCCTGTTCTTCGAGGGCGATGTGCGTTTCCTGCGTCAGTTCGCGTAA

Upstream 100 bases:

>100_bases
TTGCCGGCTGATCACCCTGACGGGGCTCTCACCGAGCCCCGTTTTTTATTCCGCGCATTTCGCGGACCCGAATCACCCGA
ATTTTTTCGCTCGCCTTCCC

Downstream 100 bases:

>100_bases
GCGCCAAGCGCTCGCACTGGTACTTCATCGCCCCGTTAATCGAATTCTCTTTCCTGTCGCGCCATGCAATTTCCGGAATC
CTGGCTTCGCAGTTTCGTCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSLDLDQVVVDAQNAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPETRKTEGARINQAKSRVEEALTARRQALA
DALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAKIFGSIGFDVADGPEIESDWMNFTALNNPDNHPARSMQDTFY
IDGRDSEDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
GVYTDFLRNFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLT
MLRYGINDLRLFFEGDVRFLRQFA

Sequences:

>Translated_344_residues
MSLDLDQVVVDAQNAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPETRKTEGARINQAKSRVEEALTARRQALA
DALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAKIFGSIGFDVADGPEIESDWMNFTALNNPDNHPARSMQDTFY
IDGRDSEDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
GVYTDFLRNFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLT
MLRYGINDLRLFFEGDVRFLRQFA
>Mature_343_residues
SLDLDQVVVDAQNAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPETRKTEGARINQAKSRVEEALTARRQALAD
ALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAKIFGSIGFDVADGPEIESDWMNFTALNNPDNHPARSMQDTFYI
DGRDSEDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLKG
VYTDFLRNFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=34.0823970037453, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI5729820, Length=295, Percent_Identity=30.5084745762712, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788007, Length=339, Percent_Identity=55.4572271386431, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=33.457249070632, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=33.457249070632, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=31.496062992126, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=370, Percent_Identity=29.4594594594595, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=43.3333333333333, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18858079, Length=309, Percent_Identity=30.7443365695793, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=28.3464566929134, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_RALPJ (B2UGJ4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899540.1
- GeneID:   6286272
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1973
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38850; Mature: 38719

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLDQVVVDAQNAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPETRKTEGAR
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCCH
INQAKSRVEEALTARRQALADALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIESDWMNFTALNNPDNHPARSMQDTFYIDGRDSEDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEECCCCHHHHH
GVYTDFLRNFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPTVVRNM
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHC
GLDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SLDLDQVVVDAQNAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPETRKTEGAR
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCCH
INQAKSRVEEALTARRQALADALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIESDWMNFTALNNPDNHPARSMQDTFYIDGRDSEDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEECCCCHHHHH
GVYTDFLRNFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPTVVRNM
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHC
GLDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA