| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is tnpA [C]
Identifier: 187928722
GI number: 187928722
Start: 1719504
End: 1722437
Strand: Direct
Name: tnpA [C]
Synonym: Rpic_1639
Alternate gene names: 187928722
Gene position: 1719504-1722437 (Clockwise)
Preceding gene: 187928721
Following gene: 187928726
Centisome position: 43.61
GC content: 63.19
Gene sequence:
>2934_bases ATGCCGGGCTTGGAATTTCGCTATGTGGGAAGAGACTCCCTACCCACGCGTCTGCCGGAGTTCGACGTCGAGCATTACTT CGCGTTGACCGCTAGCGATGTCGCCGCGATCAACCAGAAGTTCCGACGCGCCAGCCGCCCTGGAGTCGCCATTCAGTTGG TCTTTCTGCGCGCCAGCGGCCGTACGCTGGACCAACTCAACACACTCCCCCGTCAGTTGCTGCGCTATATCGGCGAAAAA CTCGCCGTCGAGACACCAACGATCGCCTCGCTGCGCACCATTTACCAGCGATACCCGACGCTGTATGAGCATCAGGTCTG GGCCCTCCAATATCTGGGCCTGACACGCCTCGATGACGAGCAATGGGCCGGACTCGAAGCCTGGATGCGTCAGGACGCCG CCGAATCGCTGACCGTTGAGGAACTGCTTCAGCACGCGCACTACTGGTTGTACGAACGGCGCATTCTGATCCCGTCGACG CGTGCGCTGCAGGATCTGGCCCGCTCCATCTGGGCGGGCATCGAGCGGGATCTGCTGGCCATGATCGACGTGGTGGTCTC GCGCGAGCAGATCCTGCGGGCCGAGGCTGCGGTGTTTGCCCAGCACGCCACATCGGGCACGACGGTGCTGGAATGGCTCA AGACCCCACCCGCGCGCCACAGCCCGTCAACGATGACCGAGATGCTGGCCAAGATCAGCTTCTTGAAGGACCTCGGCGCG CATACCTGGACATTTGACGCGGTCCCCATCGAGAAACAGCGGGCCTACGGCCAACGTATCCAGGCCCGCCGTCCCGCCAA AATCCGCGAACTCAAAGCGTCGACGCGCACGCTCGAACTGGTGTTCTTCCTGCGTGTCACCTTGCTGGAGTTGACTGACG CCATGGTGTACCAAAGCGGGCGCCGTATCTCAGACCTGGTCCGGCAGGCGTACAACAAGACCACGACGAAGCAAGCCCGC TCGGCGATCGAATACCGGCAGCAACTGGTGGAGATCAAGGCCCTGGTGGACGATACCCGCCGCTCGGCCGAGGACCGACT GGCCGACATCGGCAAGCTGCTTAAGGGTCTGTCCAGTCGGCCGCCGGCAAGTCACGCCGCAAGCGTGCGCGAAACCCTTA CCGAAGACCACCACCGACTCCGCAACCTCCTGACACCCTTGCGGGCGTTGGAATTCGCCAGTCACGGTAACGATCCTGCC ATGCGCCAACTCGATCTCGTTGGCGGTTTGCACGATCGGGGCATGACGGAGTTGCCGCCAGACTGCAATGTGCCAGTGAG CGCCAGTTGGCGCGACCTGGTCGACGGTGAGGACCGCCAGCGCGCGCTGCGTGCCTTGGAGGCCTCTGCTGTAATGAGTT TGCGCAAGGGGCTGCGCCGCGGCACGGTGTGGATCAACCATAGCCTGTCGTTTCGAGAACGAGACCAGTTGCTGATTCCG CCGGCACAGTGGGAGGTCGACCGCGACCGGCATCTGTCTTCGCTCGGCCTGCCAAGCCAAGCGAGGCTCTATCTTGATCG CCTGACCGAGCATTTGAAGGCGGGTCTCGCTGCGCTGGACGAGGCCCGTGAGGCTGGCCACTTTACGATCGACGCGGACG GCATCCTGCATTTGTCGCCATTGGAAGCGCAGACCACGGATACCACGCCTCAACGCACCCGAGATCTGCTGTTCAAGGAC ATCGGCAGCGCACAGTTTGCCGACATGATTCTGGAGATGGATGCGCGCACCAATTTCAGTGAAGTGCTGTTGGCGCGCAA GGCGCGGGATGCCCACGAACTCGTCTCCCTTTATGCAGCCCTGATCGCGCACGGCACCGAACTGGATGCCAAGGGCGTCG CGGCGATGATCCCGCAGTTGGACCTCGCGCACGTCTCCACCGCGATGCGCTCCCTGGAAATGCCCGGCCGGCTGCGCCGC GCCAACGAACGTGTGGTGGAGTTCCAGCGCACGCACCCGATCACCGATCTATGGGGCACCGGCCAACTCGCCTCCAGCGA CTCCATGAGCCTGGACACGTCCCCACATCTGTTCTACGCGCGCGCTGATCCCCGCCGACGCACGCACGCCGCTGGCGTCT ATACGCACGTGCTGGATCAGCATGGGATCGTCTACAACCAGCCGATGGTGCTCAACGAGCGCCAAACTGGCGCGGCGATC GAAGGCGTCATGCGTCACAACGAAACCCGCGCTGACGGTGGGCTGCTGCGGCTCGCCGTCGACACCCATGGCTACACGAA CGTGGGGATGGCGGTGGCCAAGCTACTTGGCTTTGACCTCTGTCCCTGGCTGCGCAACCTGTCGGAGCGCAAGCTGTACC TGCCGCGTGCCCTGTCGGTGACGGAGGGATTGTCGGCTGCCGTGTCGCACGAGGTCTCCCTCAAGGCGATCCGCGACGGC TGGGATGGCTTGCTACGCCTGGTGGCGTCCATTCACGCGGGGCGCGTGAGCGCGATCGTTGCGCTGCAACGCTTCGGCAG TGCTGCCCAGGGTGACCCGATCCACCGGGCAGCGGATCACCTGGGCAAGCTGCTGCGCACGCTGTTCCTATGCGACTTTT TCAGCAACATCGAGTTTCGTCGCGAGTTGCGCACGCTCCTCAATCGGGGTGAATCCGTGCACCATCTCCAGCGGGCGATT TATATGAGTAAGGTCCAACACGAGCGCGGTCGCCGCCAGGACGAGATGATCGCGATTTCCGGGGCGCTCTCGCTGCTGAC CAATCTGGTGATTGCCTGGAATACCGAGCGGATGCAAACGACGGTGGATACGTGGCAGCGCAAGGGGCAACGGATCGACG ATGACTGGCTGCGCCGCATGGGGCCGGCACACTTCGCCCATGTGAATTTCCGGGGCGTTTTGAGCTTCAAGATCCACCAA TTTCAGGATGCCCTGTTGGAGGCGGCACCTCGCCGGCGTGCGAGCCAAGCATGA
Upstream 100 bases:
>100_bases CCCAGGTCTCTGCCGACAATTGTCTGCTGCCCTCCAGTGACTTCGGGAGTACGATATTCGCCCGCGATTTCGGATCGCGC CACCTTGACGGGGGCACAAG
Downstream 100 bases:
>100_bases AGTCCTGCTTGGCACGGGTCGGCCGCAATCGCGTTTATTAGAGGTTCATGCAAGGTTACTGCGTCAAAGCAATGGCAGAG GTCCGCCAGACCGGACCTGT
Product: transposase Tn3 family protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 977; Mature: 976
Protein sequence:
>977_residues MPGLEFRYVGRDSLPTRLPEFDVEHYFALTASDVAAINQKFRRASRPGVAIQLVFLRASGRTLDQLNTLPRQLLRYIGEK LAVETPTIASLRTIYQRYPTLYEHQVWALQYLGLTRLDDEQWAGLEAWMRQDAAESLTVEELLQHAHYWLYERRILIPST RALQDLARSIWAGIERDLLAMIDVVVSREQILRAEAAVFAQHATSGTTVLEWLKTPPARHSPSTMTEMLAKISFLKDLGA HTWTFDAVPIEKQRAYGQRIQARRPAKIRELKASTRTLELVFFLRVTLLELTDAMVYQSGRRISDLVRQAYNKTTTKQAR SAIEYRQQLVEIKALVDDTRRSAEDRLADIGKLLKGLSSRPPASHAASVRETLTEDHHRLRNLLTPLRALEFASHGNDPA MRQLDLVGGLHDRGMTELPPDCNVPVSASWRDLVDGEDRQRALRALEASAVMSLRKGLRRGTVWINHSLSFRERDQLLIP PAQWEVDRDRHLSSLGLPSQARLYLDRLTEHLKAGLAALDEAREAGHFTIDADGILHLSPLEAQTTDTTPQRTRDLLFKD IGSAQFADMILEMDARTNFSEVLLARKARDAHELVSLYAALIAHGTELDAKGVAAMIPQLDLAHVSTAMRSLEMPGRLRR ANERVVEFQRTHPITDLWGTGQLASSDSMSLDTSPHLFYARADPRRRTHAAGVYTHVLDQHGIVYNQPMVLNERQTGAAI EGVMRHNETRADGGLLRLAVDTHGYTNVGMAVAKLLGFDLCPWLRNLSERKLYLPRALSVTEGLSAAVSHEVSLKAIRDG WDGLLRLVASIHAGRVSAIVALQRFGSAAQGDPIHRAADHLGKLLRTLFLCDFFSNIEFRRELRTLLNRGESVHHLQRAI YMSKVQHERGRRQDEMIAISGALSLLTNLVIAWNTERMQTTVDTWQRKGQRIDDDWLRRMGPAHFAHVNFRGVLSFKIHQ FQDALLEAAPRRRASQA
Sequences:
>Translated_977_residues MPGLEFRYVGRDSLPTRLPEFDVEHYFALTASDVAAINQKFRRASRPGVAIQLVFLRASGRTLDQLNTLPRQLLRYIGEK LAVETPTIASLRTIYQRYPTLYEHQVWALQYLGLTRLDDEQWAGLEAWMRQDAAESLTVEELLQHAHYWLYERRILIPST RALQDLARSIWAGIERDLLAMIDVVVSREQILRAEAAVFAQHATSGTTVLEWLKTPPARHSPSTMTEMLAKISFLKDLGA HTWTFDAVPIEKQRAYGQRIQARRPAKIRELKASTRTLELVFFLRVTLLELTDAMVYQSGRRISDLVRQAYNKTTTKQAR SAIEYRQQLVEIKALVDDTRRSAEDRLADIGKLLKGLSSRPPASHAASVRETLTEDHHRLRNLLTPLRALEFASHGNDPA MRQLDLVGGLHDRGMTELPPDCNVPVSASWRDLVDGEDRQRALRALEASAVMSLRKGLRRGTVWINHSLSFRERDQLLIP PAQWEVDRDRHLSSLGLPSQARLYLDRLTEHLKAGLAALDEAREAGHFTIDADGILHLSPLEAQTTDTTPQRTRDLLFKD IGSAQFADMILEMDARTNFSEVLLARKARDAHELVSLYAALIAHGTELDAKGVAAMIPQLDLAHVSTAMRSLEMPGRLRR ANERVVEFQRTHPITDLWGTGQLASSDSMSLDTSPHLFYARADPRRRTHAAGVYTHVLDQHGIVYNQPMVLNERQTGAAI EGVMRHNETRADGGLLRLAVDTHGYTNVGMAVAKLLGFDLCPWLRNLSERKLYLPRALSVTEGLSAAVSHEVSLKAIRDG WDGLLRLVASIHAGRVSAIVALQRFGSAAQGDPIHRAADHLGKLLRTLFLCDFFSNIEFRRELRTLLNRGESVHHLQRAI YMSKVQHERGRRQDEMIAISGALSLLTNLVIAWNTERMQTTVDTWQRKGQRIDDDWLRRMGPAHFAHVNFRGVLSFKIHQ FQDALLEAAPRRRASQA >Mature_976_residues PGLEFRYVGRDSLPTRLPEFDVEHYFALTASDVAAINQKFRRASRPGVAIQLVFLRASGRTLDQLNTLPRQLLRYIGEKL AVETPTIASLRTIYQRYPTLYEHQVWALQYLGLTRLDDEQWAGLEAWMRQDAAESLTVEELLQHAHYWLYERRILIPSTR ALQDLARSIWAGIERDLLAMIDVVVSREQILRAEAAVFAQHATSGTTVLEWLKTPPARHSPSTMTEMLAKISFLKDLGAH TWTFDAVPIEKQRAYGQRIQARRPAKIRELKASTRTLELVFFLRVTLLELTDAMVYQSGRRISDLVRQAYNKTTTKQARS AIEYRQQLVEIKALVDDTRRSAEDRLADIGKLLKGLSSRPPASHAASVRETLTEDHHRLRNLLTPLRALEFASHGNDPAM RQLDLVGGLHDRGMTELPPDCNVPVSASWRDLVDGEDRQRALRALEASAVMSLRKGLRRGTVWINHSLSFRERDQLLIPP AQWEVDRDRHLSSLGLPSQARLYLDRLTEHLKAGLAALDEAREAGHFTIDADGILHLSPLEAQTTDTTPQRTRDLLFKDI GSAQFADMILEMDARTNFSEVLLARKARDAHELVSLYAALIAHGTELDAKGVAAMIPQLDLAHVSTAMRSLEMPGRLRRA NERVVEFQRTHPITDLWGTGQLASSDSMSLDTSPHLFYARADPRRRTHAAGVYTHVLDQHGIVYNQPMVLNERQTGAAIE GVMRHNETRADGGLLRLAVDTHGYTNVGMAVAKLLGFDLCPWLRNLSERKLYLPRALSVTEGLSAAVSHEVSLKAIRDGW DGLLRLVASIHAGRVSAIVALQRFGSAAQGDPIHRAADHLGKLLRTLFLCDFFSNIEFRRELRTLLNRGESVHHLQRAIY MSKVQHERGRRQDEMIAISGALSLLTNLVIAWNTERMQTTVDTWQRKGQRIDDDWLRRMGPAHFAHVNFRGVLSFKIHQF QDALLEAAPRRRASQA
Specific function: Required for transposition of transposon Tn5271 [H]
COG id: COG4644
COG function: function code L; Transposase and inactivated derivatives, TnpA family
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transposase 7 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002513 [H]
Pfam domain/function: PF01526 Transposase_7 [H]
EC number: NA
Molecular weight: Translated: 110436; Mature: 110305
Theoretical pI: Translated: 9.72; Mature: 9.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPGLEFRYVGRDSLPTRLPEFDVEHYFALTASDVAAINQKFRRASRPGVAIQLVFLRASG CCCCCEEECCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC RTLDQLNTLPRQLLRYIGEKLAVETPTIASLRTIYQRYPTLYEHQVWALQYLGLTRLDDE CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH QWAGLEAWMRQDAAESLTVEELLQHAHYWLYERRILIPSTRALQDLARSIWAGIERDLLA HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHH MIDVVVSREQILRAEAAVFAQHATSGTTVLEWLKTPPARHSPSTMTEMLAKISFLKDLGA HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC HTWTFDAVPIEKQRAYGQRIQARRPAKIRELKASTRTLELVFFLRVTLLELTDAMVYQSG CCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC RRISDLVRQAYNKTTTKQARSAIEYRQQLVEIKALVDDTRRSAEDRLADIGKLLKGLSSR CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC PPASHAASVRETLTEDHHRLRNLLTPLRALEFASHGNDPAMRQLDLVGGLHDRGMTELPP CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC DCNVPVSASWRDLVDGEDRQRALRALEASAVMSLRKGLRRGTVWINHSLSFRERDQLLIP CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEECC PAQWEVDRDRHLSSLGLPSQARLYLDRLTEHLKAGLAALDEAREAGHFTIDADGILHLSP CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECC LEAQTTDTTPQRTRDLLFKDIGSAQFADMILEMDARTNFSEVLLARKARDAHELVSLYAA CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHH LIAHGTELDAKGVAAMIPQLDLAHVSTAMRSLEMPGRLRRANERVVEFQRTHPITDLWGT HHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC GQLASSDSMSLDTSPHLFYARADPRRRTHAAGVYTHVLDQHGIVYNQPMVLNERQTGAAI CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCHHH EGVMRHNETRADGGLLRLAVDTHGYTNVGMAVAKLLGFDLCPWLRNLSERKLYLPRALSV HHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCHHHCEEECCCHHHH TEGLSAAVSHEVSLKAIRDGWDGLLRLVASIHAGRVSAIVALQRFGSAAQGDPIHRAADH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH LGKLLRTLFLCDFFSNIEFRRELRTLLNRGESVHHLQRAIYMSKVQHERGRRQDEMIAIS HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH GALSLLTNLVIAWNTERMQTTVDTWQRKGQRIDDDWLRRMGPAHFAHVNFRGVLSFKIHQ HHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHHH FQDALLEAAPRRRASQA HHHHHHHHHHHHCCCCC >Mature Secondary Structure PGLEFRYVGRDSLPTRLPEFDVEHYFALTASDVAAINQKFRRASRPGVAIQLVFLRASG CCCCEEECCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC RTLDQLNTLPRQLLRYIGEKLAVETPTIASLRTIYQRYPTLYEHQVWALQYLGLTRLDDE CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH QWAGLEAWMRQDAAESLTVEELLQHAHYWLYERRILIPSTRALQDLARSIWAGIERDLLA HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHH MIDVVVSREQILRAEAAVFAQHATSGTTVLEWLKTPPARHSPSTMTEMLAKISFLKDLGA HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC HTWTFDAVPIEKQRAYGQRIQARRPAKIRELKASTRTLELVFFLRVTLLELTDAMVYQSG CCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC RRISDLVRQAYNKTTTKQARSAIEYRQQLVEIKALVDDTRRSAEDRLADIGKLLKGLSSR CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC PPASHAASVRETLTEDHHRLRNLLTPLRALEFASHGNDPAMRQLDLVGGLHDRGMTELPP CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC DCNVPVSASWRDLVDGEDRQRALRALEASAVMSLRKGLRRGTVWINHSLSFRERDQLLIP CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEECC PAQWEVDRDRHLSSLGLPSQARLYLDRLTEHLKAGLAALDEAREAGHFTIDADGILHLSP CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECC LEAQTTDTTPQRTRDLLFKDIGSAQFADMILEMDARTNFSEVLLARKARDAHELVSLYAA CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHH LIAHGTELDAKGVAAMIPQLDLAHVSTAMRSLEMPGRLRRANERVVEFQRTHPITDLWGT HHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC GQLASSDSMSLDTSPHLFYARADPRRRTHAAGVYTHVLDQHGIVYNQPMVLNERQTGAAI CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCHHH EGVMRHNETRADGGLLRLAVDTHGYTNVGMAVAKLLGFDLCPWLRNLSERKLYLPRALSV HHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCHHHCEEECCCHHHH TEGLSAAVSHEVSLKAIRDGWDGLLRLVASIHAGRVSAIVALQRFGSAAQGDPIHRAADH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH LGKLLRTLFLCDFFSNIEFRRELRTLLNRGESVHHLQRAIYMSKVQHERGRRQDEMIAIS HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH GALSLLTNLVIAWNTERMQTTVDTWQRKGQRIDDDWLRRMGPAHFAHVNFRGVLSFKIHQ HHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHHH FQDALLEAAPRRRASQA HHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1656436 [H]