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The map label for this gene is nucA [H]

Identifier: 187928644

GI number: 187928644

Start: 1649092

End: 1650009

Strand: Reverse

Name: nucA [H]

Synonym: Rpic_1560

Alternate gene names: 187928644

Gene position: 1650009-1649092 (Counterclockwise)

Preceding gene: 187928645

Following gene: 187928643

Centisome position: 41.85

GC content: 66.23

Gene sequence:

>918_bases
GTGCTCAAAAAAACCCTGCTCTTCGTTGCCCTCTCTGCATTCCTCCAGGTCGCCGGCGCCTCTCCCGATCACTGCGGCGC
ATCGGTCGCGGACGGCGCGCAACCGAAGGTGCTGTCCAGCAAGCACGCTGGCGCCAGCCACCTGCTCTGCTACCGCGCCT
ACGCGGTGCTCGTGTCCTCGGCGACCCGGACACCGCTGTGGTCAGCCGAACACCTGACCGCGCAGGCGGTGAACCAGGCG
CGTGAGCTGCCGCGCGAAAGCGATTTCTTCGAAGAGACGTCTTTGCCGGCAACCGCCCGCGCCACCCTGCAGGACTACCA
CGGTAGCTCGCACGTCCAGATTGACCGTGGCCACATGTCGCCAAGCGGGGACTTCGGCGACCTGGCATCGCAACAGGAAA
GTTTCAGCTTGGCCAACGTCGTCCCGCAGAACTCGGTCAGCAACCGCCGCATGTGGAGCCACCTGGAGACGTCGACCCGC
CGCCTGGTACGGCAGACCGGCGAAGCCTACGTGGTGACCGGCCCAGCATTCACGGACGGCAAGGCCCGTTACCTCAACGA
ACATGTACGGGTACCCGACTATCTGTGGAAAGCGATCTACGTCCCGGGCGTCGGCGCAGCAGCCTACATCGCACCCAACG
ATGCCACGCCGAAATATGCGGTGGTCAGCATCGCCAAACTCGCACAATTCACCGGCGTCGACCCGTTCCCCCGGCTGCCG
GCCGCGACGCGCGATCAGGCGATGGAGTTGCCGCCGCCCACCCCGCATCCGCACGAGAAGGCTGGTCGGCAGACCACCCT
CAACGATCTCTTGAGCCGCGCGCCGGCAGCGGAGGTGGAAGATGTCGGACCGACTGCCGTGGCCCGCGTTCCCAACGTAT
GGCGGCTGGCCCGCGCAATCAGCGCCGCCGTTCGCTAA

Upstream 100 bases:

>100_bases
CCTTCACGCAAATCGGCCCGCAGAAGCTCTCGCTTTCGGGTACTTGACATTGCCCGTTACAAGGCACGATGGATCTAGGT
CAACCCGGATCCCAACTGCC

Downstream 100 bases:

>100_bases
GATGGAATCTAAATGGAGGCATCCCATGAAAGCCCAACAACCGTACGGATCTCCCCAAACCCTGGGCCGTCGTGTCAGCT
TCTGGCTGATCGCTGTGGCC

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLKKTLLFVALSAFLQVAGASPDHCGASVADGAQPKVLSSKHAGASHLLCYRAYAVLVSSATRTPLWSAEHLTAQAVNQA
RELPRESDFFEETSLPATARATLQDYHGSSHVQIDRGHMSPSGDFGDLASQQESFSLANVVPQNSVSNRRMWSHLETSTR
RLVRQTGEAYVVTGPAFTDGKARYLNEHVRVPDYLWKAIYVPGVGAAAYIAPNDATPKYAVVSIAKLAQFTGVDPFPRLP
AATRDQAMELPPPTPHPHEKAGRQTTLNDLLSRAPAAEVEDVGPTAVARVPNVWRLARAISAAVR

Sequences:

>Translated_305_residues
MLKKTLLFVALSAFLQVAGASPDHCGASVADGAQPKVLSSKHAGASHLLCYRAYAVLVSSATRTPLWSAEHLTAQAVNQA
RELPRESDFFEETSLPATARATLQDYHGSSHVQIDRGHMSPSGDFGDLASQQESFSLANVVPQNSVSNRRMWSHLETSTR
RLVRQTGEAYVVTGPAFTDGKARYLNEHVRVPDYLWKAIYVPGVGAAAYIAPNDATPKYAVVSIAKLAQFTGVDPFPRLP
AATRDQAMELPPPTPHPHEKAGRQTTLNDLLSRAPAAEVEDVGPTAVARVPNVWRLARAISAAVR
>Mature_305_residues
MLKKTLLFVALSAFLQVAGASPDHCGASVADGAQPKVLSSKHAGASHLLCYRAYAVLVSSATRTPLWSAEHLTAQAVNQA
RELPRESDFFEETSLPATARATLQDYHGSSHVQIDRGHMSPSGDFGDLASQQESFSLANVVPQNSVSNRRMWSHLETSTR
RLVRQTGEAYVVTGPAFTDGKARYLNEHVRVPDYLWKAIYVPGVGAAAYIAPNDATPKYAVVSIAKLAQFTGVDPFPRLP
AATRDQAMELPPPTPHPHEKAGRQTTLNDLLSRAPAAEVEDVGPTAVARVPNVWRLARAISAAVR

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI53759134, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI224451073, Length=214, Percent_Identity=28.0373831775701, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17505967, Length=197, Percent_Identity=28.4263959390863, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322253, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129899, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581065, Length=232, Percent_Identity=24.5689655172414, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: NA

Molecular weight: Translated: 32909; Mature: 32909

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTLLFVALSAFLQVAGASPDHCGASVADGAQPKVLSSKHAGASHLLCYRAYAVLVSS
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
ATRTPLWSAEHLTAQAVNQARELPRESDFFEETSLPATARATLQDYHGSSHVQIDRGHMS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCC
PSGDFGDLASQQESFSLANVVPQNSVSNRRMWSHLETSTRRLVRQTGEAYVVTGPAFTDG
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
KARYLNEHVRVPDYLWKAIYVPGVGAAAYIAPNDATPKYAVVSIAKLAQFTGVDPFPRLP
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
AATRDQAMELPPPTPHPHEKAGRQTTLNDLLSRAPAAEVEDVGPTAVARVPNVWRLARAI
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHCCCHHHHHCCHHHHHHHHH
SAAVR
HHHCC
>Mature Secondary Structure
MLKKTLLFVALSAFLQVAGASPDHCGASVADGAQPKVLSSKHAGASHLLCYRAYAVLVSS
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
ATRTPLWSAEHLTAQAVNQARELPRESDFFEETSLPATARATLQDYHGSSHVQIDRGHMS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCC
PSGDFGDLASQQESFSLANVVPQNSVSNRRMWSHLETSTRRLVRQTGEAYVVTGPAFTDG
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
KARYLNEHVRVPDYLWKAIYVPGVGAAAYIAPNDATPKYAVVSIAKLAQFTGVDPFPRLP
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
AATRDQAMELPPPTPHPHEKAGRQTTLNDLLSRAPAAEVEDVGPTAVARVPNVWRLARAI
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHCCCHHHHHCCHHHHHHHHH
SAAVR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]