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The map label for this gene is bfpB [H]

Identifier: 187928617

GI number: 187928617

Start: 1624985

End: 1626676

Strand: Direct

Name: bfpB [H]

Synonym: Rpic_1533

Alternate gene names: 187928617

Gene position: 1624985-1626676 (Clockwise)

Preceding gene: 187928616

Following gene: 187928618

Centisome position: 41.22

GC content: 61.35

Gene sequence:

>1692_bases
ATGAGGATCTTCTATCAACCGAAGCTGATCGCCGGTGCTGTGGCAACGGTCTTGAGTCTGTCGGGATGCGGTATCGCGGA
GGTGCGCGGCATCCAGAAAGACGCGATGCGCGACACGCGCGCGCAGCTCGACGCTGCCCCGAGCAGCCGCCCGGTCTTCC
AGATCCATGAGGGGGCATGGCTTCTTGGCGAGAAGGTCAAGGCGACCAAGCCGCAACCGGAACTCTTCAACAAGCCCGTG
TCGTACAACGACGCACGGGACCGCATCAGTACGCTTGCCGACGTTGCCGACTGGATTGCTCGCACTTACAACGTACGGGT
GGTCATCGATCCCTCGGTGAATGCACCGTTCCCCTCGGCGCAGTCCATTGTGACCACCGGCGCAACCGGCGCAGTCAAGG
GGCCGCAACTACCCGCAGGGCTCGCCACGAGTCTTGCAGGCGCGCCCAACGCAACGTTGGCCGCCCCAGGTGGCATGCAG
TCTGGGGCCGCAGCGATGCCGACGCAGTCACCGCTGCACTACGTTGGCGATTTCAAGGGTTTTATGCGGACGAACGAGCA
GCGCTACAACGTCTACTCGCGCTTTCGCGACGGCACCCTCACGTTCTTCCGAGTCGAGACCCGGAACTTCACGTTGCCTG
ACATGGGAGCCTTCGCTTCCATGAGCGGGACGATCTCCAGTGACGGCAACCTGTCTTCCAGCTCGGGCGGCACGTCGGCT
TCTACCACTAGCTCCACATCTGGCGGTTCAAGCGGTTCGGGCACCGGCGGACAAACAGCCAGCCAGTCGTTGGAAGTGAA
ACCGTGGAAACGCTTGGAGGAGATCGCCAAGCAAATTGCGGGGAATGGTGCGGAGGTCATCGCCGACCCGAATCTGGGGA
CTGTCACTGTCACCGGGACGCCGCCACAGTGTGATCGTGTTGAGGACTGGGTCCGTACTCTTGATGCCATGTTCGGTAAG
CAGGTCGCCATCGACGTGCGCATCTACGAAGTCCGGCTCAATCAAGAGGACAACTACGGCTTGAATCTCTCGCTCGGGTA
CAAGAGCGGAAATGGGCACACCGGTGCCACGTTCACCGGGGTGGCGCCGCCAACGGTGTCGAGCAACGCGACACCCATGT
CCGTCGGCGCGACGATTGTCGGCGGCAAGCTGGACGGTACCAAGGTGGCAGTGCAAGCGCTGTCGACGCTGGGCAACGTT
ACGCAGGTTGTGTCGCGTTCGGGAGTTACCCAAAACGGCAAGGTCATGGCAATGCAGTCTGCAACTTTGCAGGACTACAT
CCCGAGCTCGCAGACCACGTTGGCGTCGAACGTGGGTTCCACGTCTTCCATGCAAACCGCCCCTGACATCTCCGGCTTCA
CAAGCACGTTCACGCCAAAGGTTGTCAACGGCCGCATCTTGATCATGTTCGACATGACCCTGTCGAATCTGAACCCGCTA
CAAGAACAGACCCAGGGCAGCGGGGCCTCCTTGTCCAAGGTGCAGCTGCGAACCAAGCCACTGGCACGTTTCCAGCAGAC
CGTCGGCCTGAAGCCGGGCGAGTCGCTGGTACTTACCGGCTTGCGCGATCAAACGACCAGTACCACGAACAACGGCGTTG
GCTCGCCCTACGTCCCAGTGACGGGCGGTGGGGTGGATGCCGCAAAGCGCGAAACGATGATTGCCGTCGTTATCACCGCA
CGGCTTCTTTGA

Upstream 100 bases:

>100_bases
GCGTCACGGAAGACTTGGCAGCCAACGGAACAGACGTGCTCGGCGACAGCTGGCGCGGCAACCACACGATCGTTATCACC
CAGAACGGAGCAGCTGAGCA

Downstream 100 bases:

>100_bases
TACCTGGGGTTCATGATGAATCGACATTTCTTGTCTGCAGCGGTTGGACTTGCGCTGAGCGCCGGTGCAGGATTTGCAAT
TGCGCAGGGTGCCTCGGCGC

Product: PilN

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MRIFYQPKLIAGAVATVLSLSGCGIAEVRGIQKDAMRDTRAQLDAAPSSRPVFQIHEGAWLLGEKVKATKPQPELFNKPV
SYNDARDRISTLADVADWIARTYNVRVVIDPSVNAPFPSAQSIVTTGATGAVKGPQLPAGLATSLAGAPNATLAAPGGMQ
SGAAAMPTQSPLHYVGDFKGFMRTNEQRYNVYSRFRDGTLTFFRVETRNFTLPDMGAFASMSGTISSDGNLSSSSGGTSA
STTSSTSGGSSGSGTGGQTASQSLEVKPWKRLEEIAKQIAGNGAEVIADPNLGTVTVTGTPPQCDRVEDWVRTLDAMFGK
QVAIDVRIYEVRLNQEDNYGLNLSLGYKSGNGHTGATFTGVAPPTVSSNATPMSVGATIVGGKLDGTKVAVQALSTLGNV
TQVVSRSGVTQNGKVMAMQSATLQDYIPSSQTTLASNVGSTSSMQTAPDISGFTSTFTPKVVNGRILIMFDMTLSNLNPL
QEQTQGSGASLSKVQLRTKPLARFQQTVGLKPGESLVLTGLRDQTTSTTNNGVGSPYVPVTGGGVDAAKRETMIAVVITA
RLL

Sequences:

>Translated_563_residues
MRIFYQPKLIAGAVATVLSLSGCGIAEVRGIQKDAMRDTRAQLDAAPSSRPVFQIHEGAWLLGEKVKATKPQPELFNKPV
SYNDARDRISTLADVADWIARTYNVRVVIDPSVNAPFPSAQSIVTTGATGAVKGPQLPAGLATSLAGAPNATLAAPGGMQ
SGAAAMPTQSPLHYVGDFKGFMRTNEQRYNVYSRFRDGTLTFFRVETRNFTLPDMGAFASMSGTISSDGNLSSSSGGTSA
STTSSTSGGSSGSGTGGQTASQSLEVKPWKRLEEIAKQIAGNGAEVIADPNLGTVTVTGTPPQCDRVEDWVRTLDAMFGK
QVAIDVRIYEVRLNQEDNYGLNLSLGYKSGNGHTGATFTGVAPPTVSSNATPMSVGATIVGGKLDGTKVAVQALSTLGNV
TQVVSRSGVTQNGKVMAMQSATLQDYIPSSQTTLASNVGSTSSMQTAPDISGFTSTFTPKVVNGRILIMFDMTLSNLNPL
QEQTQGSGASLSKVQLRTKPLARFQQTVGLKPGESLVLTGLRDQTTSTTNNGVGSPYVPVTGGGVDAAKRETMIAVVITA
RLL
>Mature_563_residues
MRIFYQPKLIAGAVATVLSLSGCGIAEVRGIQKDAMRDTRAQLDAAPSSRPVFQIHEGAWLLGEKVKATKPQPELFNKPV
SYNDARDRISTLADVADWIARTYNVRVVIDPSVNAPFPSAQSIVTTGATGAVKGPQLPAGLATSLAGAPNATLAAPGGMQ
SGAAAMPTQSPLHYVGDFKGFMRTNEQRYNVYSRFRDGTLTFFRVETRNFTLPDMGAFASMSGTISSDGNLSSSSGGTSA
STTSSTSGGSSGSGTGGQTASQSLEVKPWKRLEEIAKQIAGNGAEVIADPNLGTVTVTGTPPQCDRVEDWVRTLDAMFGK
QVAIDVRIYEVRLNQEDNYGLNLSLGYKSGNGHTGATFTGVAPPTVSSNATPMSVGATIVGGKLDGTKVAVQALSTLGNV
TQVVSRSGVTQNGKVMAMQSATLQDYIPSSQTTLASNVGSTSSMQTAPDISGFTSTFTPKVVNGRILIMFDMTLSNLNPL
QEQTQGSGASLSKVQLRTKPLARFQQTVGLKPGESLVLTGLRDQTTSTTNNGVGSPYVPVTGGGVDAAKRETMIAVVITA
RLL

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 58834; Mature: 58834

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFYQPKLIAGAVATVLSLSGCGIAEVRGIQKDAMRDTRAQLDAAPSSRPVFQIHEGAW
CEEEECCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCE
LLGEKVKATKPQPELFNKPVSYNDARDRISTLADVADWIARTYNVRVVIDPSVNAPFPSA
EECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
QSIVTTGATGAVKGPQLPAGLATSLAGAPNATLAAPGGMQSGAAAMPTQSPLHYVGDFKG
CCEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
FMRTNEQRYNVYSRFRDGTLTFFRVETRNFTLPDMGAFASMSGTISSDGNLSSSSGGTSA
HHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
STTSSTSGGSSGSGTGGQTASQSLEVKPWKRLEEIAKQIAGNGAEVIADPNLGTVTVTGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECC
PPQCDRVEDWVRTLDAMFGKQVAIDVRIYEVRLNQEDNYGLNLSLGYKSGNGHTGATFTG
CCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEC
VAPPTVSSNATPMSVGATIVGGKLDGTKVAVQALSTLGNVTQVVSRSGVTQNGKVMAMQS
CCCCCCCCCCCCEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
ATLQDYIPSSQTTLASNVGSTSSMQTAPDISGFTSTFTPKVVNGRILIMFDMTLSNLNPL
CHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCCCHH
QEQTQGSGASLSKVQLRTKPLARFQQTVGLKPGESLVLTGLRDQTTSTTNNGVGSPYVPV
HHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEE
TGGGVDAAKRETMIAVVITARLL
ECCCCCCHHHCEEEEEEEEECCC
>Mature Secondary Structure
MRIFYQPKLIAGAVATVLSLSGCGIAEVRGIQKDAMRDTRAQLDAAPSSRPVFQIHEGAW
CEEEECCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCE
LLGEKVKATKPQPELFNKPVSYNDARDRISTLADVADWIARTYNVRVVIDPSVNAPFPSA
EECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
QSIVTTGATGAVKGPQLPAGLATSLAGAPNATLAAPGGMQSGAAAMPTQSPLHYVGDFKG
CCEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
FMRTNEQRYNVYSRFRDGTLTFFRVETRNFTLPDMGAFASMSGTISSDGNLSSSSGGTSA
HHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
STTSSTSGGSSGSGTGGQTASQSLEVKPWKRLEEIAKQIAGNGAEVIADPNLGTVTVTGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECC
PPQCDRVEDWVRTLDAMFGKQVAIDVRIYEVRLNQEDNYGLNLSLGYKSGNGHTGATFTG
CCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEC
VAPPTVSSNATPMSVGATIVGGKLDGTKVAVQALSTLGNVTQVVSRSGVTQNGKVMAMQS
CCCCCCCCCCCCEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
ATLQDYIPSSQTTLASNVGSTSSMQTAPDISGFTSTFTPKVVNGRILIMFDMTLSNLNPL
CHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCCCHH
QEQTQGSGASLSKVQLRTKPLARFQQTVGLKPGESLVLTGLRDQTTSTTNNGVGSPYVPV
HHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEE
TGGGVDAAKRETMIAVVITARLL
ECCCCCCHHHCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]