| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is virB4 [H]
Identifier: 187928606
GI number: 187928606
Start: 1614519
End: 1616966
Strand: Reverse
Name: virB4 [H]
Synonym: Rpic_1522
Alternate gene names: 187928606
Gene position: 1616966-1614519 (Counterclockwise)
Preceding gene: 187928607
Following gene: 187928605
Centisome position: 41.01
GC content: 60.66
Gene sequence:
>2448_bases ATGCTGAAGAAAAAACGGCCCATCCAGGAAATCGCTCCCTGGCTCACCTTTATCACGCCCTCGCTGGTGCTCGGCAAGGA TGGCAGTTTGCTGGCCACCTACGGGCTGGGGGGCGTTGACGCGGACCACCCCAACAAGAGTGAGATCACATCCGCTCGGG ATCAGCTCGATCAGGCCTGCCGCAGCCTGGACGGTCGCATCACTGCGTGGTGGCGCATCAAGCACCGCCGCGCACACGAC TACATCGCCGGCGAGTTCGCCAGTGCTCATGACGCCAAGATCGACGAGCTCAATCGGGCCAACATGTCATCGGGCAAGTT CTACCAGAACACGCACTCGCTCGCGCTCAGCTACACGCCCGCATCCGGCCTGGACAAGATCTTCGAAAAGATCAGCTACC ACGTCACGGTGGGCGGCAAGGGGCTGGTCGCGGCGTTATTCGAGTCGCTAAAGGACACGTTCCTCGCGCGCAACGCGTTC GCATTCGATCTTGCCAAGACGCAGGCCGATGCCAAGCGCTTCGAAGGCATGCTCGATGCGTTTGTTGGCGGCAACACCAA GATCGGGCTCAAACGGCAGATGCTGCAGGAGTCCATGGCACTTCTGCATCAAGCGGCGAACCCATCGGTGCCGCCACGCA GGATTCGCTTCCCGATGACGCTGCTGGACACCCACCTCACCGAGTCGATCGTGACGCCGGGTGCCGAGCAACTGCTCTTT GAGTCGGCGCATGGAATGCGCTACGCGGCCGTGGTCGCCGTCAAGGAATGGCTCGGCTTCCAAGAAGCGGCGCTGGACGT CCTGACGAAGCTCGATGTTGAGCTGGACGTCTGCGTCCTCTACCGCTTCTTGGACAACTCCAAGGCCAACGTCTACATCG AGAAGATCCGCAGCTTCTACAAGATGGCGAGCTTCAACCTGTGGAAGATTCTGAAGCAGTTCGCTGCCAAGGAAGAACAG GAGAACGATGAGGGCCGGGAACAGCTCGCCGACGAGGCGGGTCAGGCACTTCGCCGGCTGACCGCGGATGGTCAGCAGCA TGGCTTCGCCAACATCTCCGTCATCGTCTACGGCAACACCGAAGAGGAATGCTCGGACGCGGTCAGTCTGGTCGTTGGCG CCATCAGTAACGCGGGGTTCGGCACGATTCGTGAAGGTACGAACCTGATGCCCGCCTGGAACTGCACGCTGCCCGGTCGT TGGGACAACCAGCGGCGCCTGCAATTTGTCGAAACACCGGCCGTGTCGGACGTCGCCCCGATTTGTACGATCAATCCGGG GCCGACCCGCAACGACTGGCTCTCGGACAAGGCCGGCAAGGAGATGCCACCACTCACCTGCTTGCCGACACGCCACCTGA CTGCCCAGCGCGTCGACATGCACGAGCCCGGGGGCAATGGCCACCTCTTCGTGATCGGCCCGATCGGTGCCGGTAAGTCG GTATTCCTGAATTTCCTCATGTCACAGTCGGGGCGCCATAACCCCCGGCGCATCCGGTTTGATAAGGACCGGTCGACACG CATCACCACGCAACTCGGTGGTGGTCAATTCATCGATGTCACCGGTAAGTTTGCGACCGCCACACGCTGCAACCCGCTGT CCCTGCTCGGCGAGGCGGAGAACCACGCCTACGTGACGGAATGGCTGATCTTGGCCATCGAGGACGAAGACGGATTCAGG CTCACCCCCACACAGAAGACGGACATCTTCGACGCGGTTCAGATTCTGGCCGGCTATAGCCGCGAGCAATGGACCCTGTC CTTCTTCGCCACACTGATCCACGCCGATCTGCGCGAGCGGCTGCAGCTCTGGATGAAGGGTGGTCAGTACGGTGACTTCT TCGACAACGTCGACGACGCGTTCCAAATCAGCGATGACCTGACCATCGAGATGGGTGAGCTGCTCATCAACTACGAGCGC GCGGCGGTTCTATTCCTAGACTACGCCTTCTTCCGGATCAGCAAGAGCATGGATGGTCAGCGATTCACGCTGATCGAGAT TGAAGAGGCGGGCTTCTTCTTCAAGTACGAACGCTTCTACAGGCGCTTGGAAACGTGGCTGACCACCATCCGCAAGCTCA ACGGTGCCGTGTGGATGGCAACACAGTCGCTGCGCCAGATCGCGCGGATTACCGACTTCGAGGTGCTGAAGGAAACCATC GCGAACTTCATCTACCTGCCCAACAGCCTCGCCAATACCAGCAAGAACCTGTACTGCGACACGTTCGGCCTGACCGTCGA CCAGGTGCAAATGATCAACGACGCCGTGCCGAACCGCGACTACCTGTGGGTCACCCGGACCACGTCGCGCATGCTCCAGA CGGACTTCAAGCCTGAGATGGTTGCCCGGCTGCGCTCCGACGGCAAGGCGCAAGCGGTTTTCGACAAGCACTACATGCAG GGCGATGGCTGGGAGGACCGCTACGTGCGTGAACTTCTGGCCGCCTAA
Upstream 100 bases:
>100_bases TGGTCTATGACCGCTATGGCGACTTGTACGAGCCAGGCCCCTGGCACGGCAAGCTCGGCGCGCGCTTTCGCCGGCCCTAC GGGTTCGACAGTGATCTGCC
Downstream 100 bases:
>100_bases GCCGAAGTCGTACCACGGCGCTGCGTTGCGCCATCTCTCAATCGAAGGAGCCACCCATGCTCAAGACCACATTCTCTGCA ACTTTGCGAAAGATGCGCGG
Product: type IV secretory pathway VirB4 components-like protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 815; Mature: 815
Protein sequence:
>815_residues MLKKKRPIQEIAPWLTFITPSLVLGKDGSLLATYGLGGVDADHPNKSEITSARDQLDQACRSLDGRITAWWRIKHRRAHD YIAGEFASAHDAKIDELNRANMSSGKFYQNTHSLALSYTPASGLDKIFEKISYHVTVGGKGLVAALFESLKDTFLARNAF AFDLAKTQADAKRFEGMLDAFVGGNTKIGLKRQMLQESMALLHQAANPSVPPRRIRFPMTLLDTHLTESIVTPGAEQLLF ESAHGMRYAAVVAVKEWLGFQEAALDVLTKLDVELDVCVLYRFLDNSKANVYIEKIRSFYKMASFNLWKILKQFAAKEEQ ENDEGREQLADEAGQALRRLTADGQQHGFANISVIVYGNTEEECSDAVSLVVGAISNAGFGTIREGTNLMPAWNCTLPGR WDNQRRLQFVETPAVSDVAPICTINPGPTRNDWLSDKAGKEMPPLTCLPTRHLTAQRVDMHEPGGNGHLFVIGPIGAGKS VFLNFLMSQSGRHNPRRIRFDKDRSTRITTQLGGGQFIDVTGKFATATRCNPLSLLGEAENHAYVTEWLILAIEDEDGFR LTPTQKTDIFDAVQILAGYSREQWTLSFFATLIHADLRERLQLWMKGGQYGDFFDNVDDAFQISDDLTIEMGELLINYER AAVLFLDYAFFRISKSMDGQRFTLIEIEEAGFFFKYERFYRRLETWLTTIRKLNGAVWMATQSLRQIARITDFEVLKETI ANFIYLPNSLANTSKNLYCDTFGLTVDQVQMINDAVPNRDYLWVTRTTSRMLQTDFKPEMVARLRSDGKAQAVFDKHYMQ GDGWEDRYVRELLAA
Sequences:
>Translated_815_residues MLKKKRPIQEIAPWLTFITPSLVLGKDGSLLATYGLGGVDADHPNKSEITSARDQLDQACRSLDGRITAWWRIKHRRAHD YIAGEFASAHDAKIDELNRANMSSGKFYQNTHSLALSYTPASGLDKIFEKISYHVTVGGKGLVAALFESLKDTFLARNAF AFDLAKTQADAKRFEGMLDAFVGGNTKIGLKRQMLQESMALLHQAANPSVPPRRIRFPMTLLDTHLTESIVTPGAEQLLF ESAHGMRYAAVVAVKEWLGFQEAALDVLTKLDVELDVCVLYRFLDNSKANVYIEKIRSFYKMASFNLWKILKQFAAKEEQ ENDEGREQLADEAGQALRRLTADGQQHGFANISVIVYGNTEEECSDAVSLVVGAISNAGFGTIREGTNLMPAWNCTLPGR WDNQRRLQFVETPAVSDVAPICTINPGPTRNDWLSDKAGKEMPPLTCLPTRHLTAQRVDMHEPGGNGHLFVIGPIGAGKS VFLNFLMSQSGRHNPRRIRFDKDRSTRITTQLGGGQFIDVTGKFATATRCNPLSLLGEAENHAYVTEWLILAIEDEDGFR LTPTQKTDIFDAVQILAGYSREQWTLSFFATLIHADLRERLQLWMKGGQYGDFFDNVDDAFQISDDLTIEMGELLINYER AAVLFLDYAFFRISKSMDGQRFTLIEIEEAGFFFKYERFYRRLETWLTTIRKLNGAVWMATQSLRQIARITDFEVLKETI ANFIYLPNSLANTSKNLYCDTFGLTVDQVQMINDAVPNRDYLWVTRTTSRMLQTDFKPEMVARLRSDGKAQAVFDKHYMQ GDGWEDRYVRELLAA >Mature_815_residues MLKKKRPIQEIAPWLTFITPSLVLGKDGSLLATYGLGGVDADHPNKSEITSARDQLDQACRSLDGRITAWWRIKHRRAHD YIAGEFASAHDAKIDELNRANMSSGKFYQNTHSLALSYTPASGLDKIFEKISYHVTVGGKGLVAALFESLKDTFLARNAF AFDLAKTQADAKRFEGMLDAFVGGNTKIGLKRQMLQESMALLHQAANPSVPPRRIRFPMTLLDTHLTESIVTPGAEQLLF ESAHGMRYAAVVAVKEWLGFQEAALDVLTKLDVELDVCVLYRFLDNSKANVYIEKIRSFYKMASFNLWKILKQFAAKEEQ ENDEGREQLADEAGQALRRLTADGQQHGFANISVIVYGNTEEECSDAVSLVVGAISNAGFGTIREGTNLMPAWNCTLPGR WDNQRRLQFVETPAVSDVAPICTINPGPTRNDWLSDKAGKEMPPLTCLPTRHLTAQRVDMHEPGGNGHLFVIGPIGAGKS VFLNFLMSQSGRHNPRRIRFDKDRSTRITTQLGGGQFIDVTGKFATATRCNPLSLLGEAENHAYVTEWLILAIEDEDGFR LTPTQKTDIFDAVQILAGYSREQWTLSFFATLIHADLRERLQLWMKGGQYGDFFDNVDDAFQISDDLTIEMGELLINYER AAVLFLDYAFFRISKSMDGQRFTLIEIEEAGFFFKYERFYRRLETWLTTIRKLNGAVWMATQSLRQIARITDFEVLKETI ANFIYLPNSLANTSKNLYCDTFGLTVDQVQMINDAVPNRDYLWVTRTTSRMLQTDFKPEMVARLRSDGKAQAVFDKHYMQ GDGWEDRYVRELLAA
Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-
COG id: COG3451
COG function: function code U; Type IV secretory pathway, VirB4 components
Gene ontology:
Cell location: Cell inner membrane (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the trbE/virB4 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR004346 - InterPro: IPR018145 [H]
Pfam domain/function: PF03135 CagE_TrbE_VirB [H]
EC number: NA
Molecular weight: Translated: 91974; Mature: 91974
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLKKKRPIQEIAPWLTFITPSLVLGKDGSLLATYGLGGVDADHPNKSEITSARDQLDQAC CCCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH RSLDGRITAWWRIKHRRAHDYIAGEFASAHDAKIDELNRANMSSGKFYQNTHSLALSYTP HHCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCCCEEEEECC ASGLDKIFEKISYHVTVGGKGLVAALFESLKDTFLARNAFAFDLAKTQADAKRFEGMLDA CHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH FVGGNTKIGLKRQMLQESMALLHQAANPSVPPRRIRFPMTLLDTHLTESIVTPGAEQLLF HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHH ESAHGMRYAAVVAVKEWLGFQEAALDVLTKLDVELDVCVLYRFLDNSKANVYIEKIRSFY HHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH KMASFNLWKILKQFAAKEEQENDEGREQLADEAGQALRRLTADGQQHGFANISVIVYGNT HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC EEECSDAVSLVVGAISNAGFGTIREGTNLMPAWNCTLPGRWDNQRRLQFVETPAVSDVAP HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCC ICTINPGPTRNDWLSDKAGKEMPPLTCLPTRHLTAQRVDMHEPGGNGHLFVIGPIGAGKS EEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCEEEEEEECCCCCHH VFLNFLMSQSGRHNPRRIRFDKDRSTRITTQLGGGQFIDVTGKFATATRCNPLSLLGEAE HHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCEEECCCCCHHHHHCCCC NHAYVTEWLILAIEDEDGFRLTPTQKTDIFDAVQILAGYSREQWTLSFFATLIHADLRER CCEEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH LQLWMKGGQYGDFFDNVDDAFQISDDLTIEMGELLINYERAAVLFLDYAFFRISKSMDGQ HHHHHCCCCCCCHHCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC RFTLIEIEEAGFFFKYERFYRRLETWLTTIRKLNGAVWMATQSLRQIARITDFEVLKETI EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHH ANFIYLPNSLANTSKNLYCDTFGLTVDQVQMINDAVPNRDYLWVTRTTSRMLQTDFKPEM HHHEECCCHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCHHH VARLRSDGKAQAVFDKHYMQGDGWEDRYVRELLAA HHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC >Mature Secondary Structure MLKKKRPIQEIAPWLTFITPSLVLGKDGSLLATYGLGGVDADHPNKSEITSARDQLDQAC CCCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH RSLDGRITAWWRIKHRRAHDYIAGEFASAHDAKIDELNRANMSSGKFYQNTHSLALSYTP HHCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCCCEEEEECC ASGLDKIFEKISYHVTVGGKGLVAALFESLKDTFLARNAFAFDLAKTQADAKRFEGMLDA CHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH FVGGNTKIGLKRQMLQESMALLHQAANPSVPPRRIRFPMTLLDTHLTESIVTPGAEQLLF HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHH ESAHGMRYAAVVAVKEWLGFQEAALDVLTKLDVELDVCVLYRFLDNSKANVYIEKIRSFY HHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH KMASFNLWKILKQFAAKEEQENDEGREQLADEAGQALRRLTADGQQHGFANISVIVYGNT HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC EEECSDAVSLVVGAISNAGFGTIREGTNLMPAWNCTLPGRWDNQRRLQFVETPAVSDVAP HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCC ICTINPGPTRNDWLSDKAGKEMPPLTCLPTRHLTAQRVDMHEPGGNGHLFVIGPIGAGKS EEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCEEEEEEECCCCCHH VFLNFLMSQSGRHNPRRIRFDKDRSTRITTQLGGGQFIDVTGKFATATRCNPLSLLGEAE HHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCEEECCCCCHHHHHCCCC NHAYVTEWLILAIEDEDGFRLTPTQKTDIFDAVQILAGYSREQWTLSFFATLIHADLRER CCEEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH LQLWMKGGQYGDFFDNVDDAFQISDDLTIEMGELLINYERAAVLFLDYAFFRISKSMDGQ HHHHHCCCCCCCHHCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC RFTLIEIEEAGFFFKYERFYRRLETWLTTIRKLNGAVWMATQSLRQIARITDFEVLKETI EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHH ANFIYLPNSLANTSKNLYCDTFGLTVDQVQMINDAVPNRDYLWVTRTTSRMLQTDFKPEM HHHEECCCHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCHHH VARLRSDGKAQAVFDKHYMQGDGWEDRYVRELLAA HHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10749166; 10882236; 7494028 [H]