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The map label for this gene is pcrA [H]

Identifier: 187928595

GI number: 187928595

Start: 1604302

End: 1606023

Strand: Reverse

Name: pcrA [H]

Synonym: Rpic_1511

Alternate gene names: 187928595

Gene position: 1606023-1604302 (Counterclockwise)

Preceding gene: 187928596

Following gene: 187928590

Centisome position: 40.74

GC content: 61.27

Gene sequence:

>1722_bases
ATGCCCACGTCAATCCTAGACGAACTGAACCCGTCACAGCGCGAAGTGGCAGACCTACGCCTGCACTGTGTCGCCGTTGC
CTGCCCGGGCGCCGGAAAGACAAAGACAATTGCGACCAAGGCAGCCATTCTTCTCGCCGATCCCGCATGCAGGGTCGGTG
CTGTGACCTTCAACAAGGAGGCGGCCACGGAACTGCAGGAGCGCATTCGCGCGCTGGCCGGGCCGGCATCGAAGGCTCGC
CTGTTGGCAGGGACCTTTCACGGGCTGGCGTTCAAGCAGCTCACACCAAAGGGATGCAAGCAGCAGGACATCGCCAACGA
GGGGGACCAGTTGGCGCTGGTTGCACAAGCCCTGAAGGAAGCCGACCTTGATTGGAAGGAAGAAGCCGCTTTGGCCGCCA
TCGAGGCAATCAAAATGGACTTCGGCAGGGTTCCGCCTGGCAGCGTTGAGGAGCGCCTGTATTCGGCATACCAACAAGCG
CTGGCGCGGAACGGGAAGCTCGACTTCCAGGACATGCTCTGTATGGCCATCGAGGGCATGGAGCGCGGAACCATCTCGCC
CTACCCTTTCACGCACCTGCTGGTCGATGAGTTCCAGGACACCGATCCGCTGCAATACCAGTGGGTCGCGCAGCACGCGC
GCAGTGGCTCGATCGTGACCGTAGTCGGTGACGATGACCAAAGCATCTACGGCTTCAGGGCCGCGCTCGGATTCCGAGGC
ATGGAGTCGTTCATCAAGGAGTTCGACGCGCGTCCCGTGATTCTGGGGGAGAACTATCGCTGCAAGTCAGAAATCCTCGC
CGCTGCCGACAACGTCATTCGGAACAACAAGGAGCGCATCCGCAAAGATCTGGTCGCCGCGCGTGGCCCCGGCGGCCACA
TCTCGTTTCGGCGCTTCGATGACGAATATGCCGAGGCCGTGAACGCAGTGGAGGCCCTCGCAGCGTCGATGCGCGCCAGC
AAATCGGCAGCGATCCTGGCAAGAACGAACCGTATCCTCGACCCAGTTGAGGCCGTCTGCCGCTCCCATGGCGTGAAGTA
TTTCCGAGCGTCCGGCAAATCAATCCTGAGCCGGGCCGAGGCCGCGCTAATGTGCAACCTGCTCGAACTCATCCAGAAAA
CGAAGACCAACGGCCTCGATGCCGTGCTGGCGTTCGCTGGCGTCGGAGCGCAAGACCTCAAGACGTTGCACAGCCGGATC
GGTCCCAGTCTTGAGCAGCGGCCGCGCAAGGAGCTGGTCGAGATGGGCCTGGCCGAGAGCACCGCGGACACGTACCGGGA
GCTGATGAAGCGGCTTGCCGAATGGCGCGGTCTCTGCGATCGGAAGTTCTATCCGCTCGTGCTGGAAGGTGTCTACGAGT
GGATGGCTCAACGTGCCAAAGCAGACACCGCCAAGCGTGCGGTCACCACGACCTACGACGTGCTGAGCCGCCTCAACGGT
CCATTCTCGGATCGTATCGCCTTCCTGCGGCGCGAGAACAACGTACCGGCGGCCGACGCGCTGATTCTCACGACCATGCA
CAACTCAAAGGGCTTGGAGTGGGATCACGTATGCATCATCAGGGCTGAAGAAACCGTGGTGCCGAACGAGAAGAGCACAG
AGTCCGAGGAGCGTCGCTTGTTCTACGTGGCCATGACCCGTGCGCGCGACCGTCTTGAGATGAGCACGGCGAAGAAGAAT
CCAACCTCTCGATTCGTTCTTGAAGCCGGTGTGTCGAAATAA

Upstream 100 bases:

>100_bases
GTGCCTGCGAGTTTCCAGAAGCTTCACCCGGCTCGCAGGCCACTTGCCGACCCCATCGGCCATCGCAGGCTCGGGGTTGG
CCACCGTATTGGTTTCTCAG

Downstream 100 bases:

>100_bases
TCAGCTAGACTTTTCCCGACAGCACGAAGTTCAGAATTTCGCCGGCATTGGGTTCGCCCCAAGTTTCGTGTGCCACCCAT
TCGAAAAAATTCGATTCCGC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MPTSILDELNPSQREVADLRLHCVAVACPGAGKTKTIATKAAILLADPACRVGAVTFNKEAATELQERIRALAGPASKAR
LLAGTFHGLAFKQLTPKGCKQQDIANEGDQLALVAQALKEADLDWKEEAALAAIEAIKMDFGRVPPGSVEERLYSAYQQA
LARNGKLDFQDMLCMAIEGMERGTISPYPFTHLLVDEFQDTDPLQYQWVAQHARSGSIVTVVGDDDQSIYGFRAALGFRG
MESFIKEFDARPVILGENYRCKSEILAAADNVIRNNKERIRKDLVAARGPGGHISFRRFDDEYAEAVNAVEALAASMRAS
KSAAILARTNRILDPVEAVCRSHGVKYFRASGKSILSRAEAALMCNLLELIQKTKTNGLDAVLAFAGVGAQDLKTLHSRI
GPSLEQRPRKELVEMGLAESTADTYRELMKRLAEWRGLCDRKFYPLVLEGVYEWMAQRAKADTAKRAVTTTYDVLSRLNG
PFSDRIAFLRRENNVPAADALILTTMHNSKGLEWDHVCIIRAEETVVPNEKSTESEERRLFYVAMTRARDRLEMSTAKKN
PTSRFVLEAGVSK

Sequences:

>Translated_573_residues
MPTSILDELNPSQREVADLRLHCVAVACPGAGKTKTIATKAAILLADPACRVGAVTFNKEAATELQERIRALAGPASKAR
LLAGTFHGLAFKQLTPKGCKQQDIANEGDQLALVAQALKEADLDWKEEAALAAIEAIKMDFGRVPPGSVEERLYSAYQQA
LARNGKLDFQDMLCMAIEGMERGTISPYPFTHLLVDEFQDTDPLQYQWVAQHARSGSIVTVVGDDDQSIYGFRAALGFRG
MESFIKEFDARPVILGENYRCKSEILAAADNVIRNNKERIRKDLVAARGPGGHISFRRFDDEYAEAVNAVEALAASMRAS
KSAAILARTNRILDPVEAVCRSHGVKYFRASGKSILSRAEAALMCNLLELIQKTKTNGLDAVLAFAGVGAQDLKTLHSRI
GPSLEQRPRKELVEMGLAESTADTYRELMKRLAEWRGLCDRKFYPLVLEGVYEWMAQRAKADTAKRAVTTTYDVLSRLNG
PFSDRIAFLRRENNVPAADALILTTMHNSKGLEWDHVCIIRAEETVVPNEKSTESEERRLFYVAMTRARDRLEMSTAKKN
PTSRFVLEAGVSK
>Mature_572_residues
PTSILDELNPSQREVADLRLHCVAVACPGAGKTKTIATKAAILLADPACRVGAVTFNKEAATELQERIRALAGPASKARL
LAGTFHGLAFKQLTPKGCKQQDIANEGDQLALVAQALKEADLDWKEEAALAAIEAIKMDFGRVPPGSVEERLYSAYQQAL
ARNGKLDFQDMLCMAIEGMERGTISPYPFTHLLVDEFQDTDPLQYQWVAQHARSGSIVTVVGDDDQSIYGFRAALGFRGM
ESFIKEFDARPVILGENYRCKSEILAAADNVIRNNKERIRKDLVAARGPGGHISFRRFDDEYAEAVNAVEALAASMRASK
SAAILARTNRILDPVEAVCRSHGVKYFRASGKSILSRAEAALMCNLLELIQKTKTNGLDAVLAFAGVGAQDLKTLHSRIG
PSLEQRPRKELVEMGLAESTADTYRELMKRLAEWRGLCDRKFYPLVLEGVYEWMAQRAKADTAKRAVTTTYDVLSRLNGP
FSDRIAFLRRENNVPAADALILTTMHNSKGLEWDHVCIIRAEETVVPNEKSTESEERRLFYVAMTRARDRLEMSTAKKNP
TSRFVLEAGVSK

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=616, Percent_Identity=30.6818181818182, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI48994965, Length=644, Percent_Identity=27.0186335403727, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322369, Length=441, Percent_Identity=27.891156462585, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 63341; Mature: 63210

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSILDELNPSQREVADLRLHCVAVACPGAGKTKTIATKAAILLADPACRVGAVTFNKE
CCCHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCHHHHHHEEEEECCCCCEEEEEECHH
AATELQERIRALAGPASKARLLAGTFHGLAFKQLTPKGCKQQDIANEGDQLALVAQALKE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHH
ADLDWKEEAALAAIEAIKMDFGRVPPGSVEERLYSAYQQALARNGKLDFQDMLCMAIEGM
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
ERGTISPYPFTHLLVDEFQDTDPLQYQWVAQHARSGSIVTVVGDDDQSIYGFRAALGFRG
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
MESFIKEFDARPVILGENYRCKSEILAAADNVIRNNKERIRKDLVAARGPGGHISFRRFD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEECC
DEYAEAVNAVEALAASMRASKSAAILARTNRILDPVEAVCRSHGVKYFRASGKSILSRAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHHH
AALMCNLLELIQKTKTNGLDAVLAFAGVGAQDLKTLHSRIGPSLEQRPRKELVEMGLAES
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TADTYRELMKRLAEWRGLCDRKFYPLVLEGVYEWMAQRAKADTAKRAVTTTYDVLSRLNG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFSDRIAFLRRENNVPAADALILTTMHNSKGLEWDHVCIIRAEETVVPNEKSTESEERRL
CHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHEE
FYVAMTRARDRLEMSTAKKNPTSRFVLEAGVSK
EEEEHHHHHHHHHHHHHCCCCCHHEEECCCCCC
>Mature Secondary Structure 
PTSILDELNPSQREVADLRLHCVAVACPGAGKTKTIATKAAILLADPACRVGAVTFNKE
CCHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCHHHHHHEEEEECCCCCEEEEEECHH
AATELQERIRALAGPASKARLLAGTFHGLAFKQLTPKGCKQQDIANEGDQLALVAQALKE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHH
ADLDWKEEAALAAIEAIKMDFGRVPPGSVEERLYSAYQQALARNGKLDFQDMLCMAIEGM
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
ERGTISPYPFTHLLVDEFQDTDPLQYQWVAQHARSGSIVTVVGDDDQSIYGFRAALGFRG
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
MESFIKEFDARPVILGENYRCKSEILAAADNVIRNNKERIRKDLVAARGPGGHISFRRFD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEECC
DEYAEAVNAVEALAASMRASKSAAILARTNRILDPVEAVCRSHGVKYFRASGKSILSRAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHHH
AALMCNLLELIQKTKTNGLDAVLAFAGVGAQDLKTLHSRIGPSLEQRPRKELVEMGLAES
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TADTYRELMKRLAEWRGLCDRKFYPLVLEGVYEWMAQRAKADTAKRAVTTTYDVLSRLNG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFSDRIAFLRRENNVPAADALILTTMHNSKGLEWDHVCIIRAEETVVPNEKSTESEERRL
CHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHEE
FYVAMTRARDRLEMSTAKKNPTSRFVLEAGVSK
EEEEHHHHHHHHHHHHHCCCCCHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]