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The map label for this gene is benM [H]

Identifier: 187928447

GI number: 187928447

Start: 1458809

End: 1459720

Strand: Reverse

Name: benM [H]

Synonym: Rpic_1360

Alternate gene names: 187928447

Gene position: 1459720-1458809 (Counterclockwise)

Preceding gene: 187928457

Following gene: 187928446

Centisome position: 37.02

GC content: 62.72

Gene sequence:

>912_bases
ATGGAGCTACGCCACCTTCGCTACTTCGTTGCCGTCGCTGAAGAGCTGAATTTCACCCGTGCCGCTGAACGGCTGCACAT
CGCGCAGCCGCCGCTCTCGCGGCAGATTCAGCAGTTGGAAGAGGAAGTCGGCGTGCTGTTGTTCGAGCGCGGCAGCCGCC
CGCTCAAGCTGACCGAAGCCGGCCGCTTCTTTCACGCGCACGCGCGGCAACTGCTCGCGCAGACGGCCGAACTCGCCTCG
ATGACACAGCGCGTCGGCCAGATCGAGCGGCGCCTGTCCATCGGCTTCGTGGCATCAACGCTGTATGGGATGCTGCCGAA
GGTCATCCGGCGCTTTCGCGCGGAGTACCCGATGGTCGATCTGACCATGCACGAGATGACGACCATGGACCAGATCCAGG
CGCTCAAGGACGGCCGCATCGATGTCGGGTTTGGGCGCATACGCTATGAAGATCCAAACGTGCGACGCATCCTCCTGCGC
GATGAACGGCTGATCGTCGCGCTGCCGTCCGGCCATGCGCTGCTGGACGGCAGGACAACCGCATCCCTGCGCGACCTTGT
CGGCGACACGCTCATCATTTACCCGCGCACACCGCGTCCGAGCTATGCCGATCAGGTGCTCGCCCTCTTCCACGATCGCG
CGCTGGAACCCAGCAAGATCTACGAGGCACGGGAATTGCAAATTGCCTTGGGGTTGGTCGCCGCAGGCGAAGGGGTATCG
GTGGTGCCGCGCAGTGTGGCGGGCCTGCGGCGCGAAGATGTCTGCTACATGGAACTCGACGATCCGCAATTGGTCTCGCC
CATTATCTTCAGCGCGCGGCTGCTCGATGAGTCTGAAGACATCCAGGCCATGCTGGCGCTGACGTATCGGTTGTACGACG
AGCAGAAAATCCCGTATTACGTGCCCTCGTAA

Upstream 100 bases:

>100_bases
ATCAGAAGAACTGCTATCAACGTGGGAACCAGATTACGCAGCGCGAATTGTCACGTCCAACACTGATTTAGTATCAGTTT
CATACTCCAGAGGTATAAGA

Downstream 100 bases:

>100_bases
GGCTTGCAATGGCGGCTCCCAAGTTACAAGCCGCTGGACACGACGGCCGCATGGGCTAGATTGAAGCAAGGCGCCCGTTC
GCCTGCGATTGGAGCGCAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MELRHLRYFVAVAEELNFTRAAERLHIAQPPLSRQIQQLEEEVGVLLFERGSRPLKLTEAGRFFHAHARQLLAQTAELAS
MTQRVGQIERRLSIGFVASTLYGMLPKVIRRFRAEYPMVDLTMHEMTTMDQIQALKDGRIDVGFGRIRYEDPNVRRILLR
DERLIVALPSGHALLDGRTTASLRDLVGDTLIIYPRTPRPSYADQVLALFHDRALEPSKIYEARELQIALGLVAAGEGVS
VVPRSVAGLRREDVCYMELDDPQLVSPIIFSARLLDESEDIQAMLALTYRLYDEQKIPYYVPS

Sequences:

>Translated_303_residues
MELRHLRYFVAVAEELNFTRAAERLHIAQPPLSRQIQQLEEEVGVLLFERGSRPLKLTEAGRFFHAHARQLLAQTAELAS
MTQRVGQIERRLSIGFVASTLYGMLPKVIRRFRAEYPMVDLTMHEMTTMDQIQALKDGRIDVGFGRIRYEDPNVRRILLR
DERLIVALPSGHALLDGRTTASLRDLVGDTLIIYPRTPRPSYADQVLALFHDRALEPSKIYEARELQIALGLVAAGEGVS
VVPRSVAGLRREDVCYMELDDPQLVSPIIFSARLLDESEDIQAMLALTYRLYDEQKIPYYVPS
>Mature_303_residues
MELRHLRYFVAVAEELNFTRAAERLHIAQPPLSRQIQQLEEEVGVLLFERGSRPLKLTEAGRFFHAHARQLLAQTAELAS
MTQRVGQIERRLSIGFVASTLYGMLPKVIRRFRAEYPMVDLTMHEMTTMDQIQALKDGRIDVGFGRIRYEDPNVRRILLR
DERLIVALPSGHALLDGRTTASLRDLVGDTLIIYPRTPRPSYADQVLALFHDRALEPSKIYEARELQIALGLVAAGEGVS
VVPRSVAGLRREDVCYMELDDPQLVSPIIFSARLLDESEDIQAMLALTYRLYDEQKIPYYVPS

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=268, Percent_Identity=35.8208955223881, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788887, Length=257, Percent_Identity=36.9649805447471, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI2367136, Length=259, Percent_Identity=32.8185328185328, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1790399, Length=266, Percent_Identity=29.3233082706767, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693105, Length=271, Percent_Identity=28.0442804428044, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787601, Length=252, Percent_Identity=29.3650793650794, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=29.3877551020408, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87081904, Length=189, Percent_Identity=34.3915343915344, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=28.0487804878049, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=29.296875, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=34.9315068493151, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790783, Length=146, Percent_Identity=30.1369863013699, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=28.2258064516129, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788481, Length=177, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=249, Percent_Identity=26.1044176706827, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=28.9795918367347, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=170, Percent_Identity=30.5882352941176, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=154, Percent_Identity=29.8701298701299, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789506, Length=248, Percent_Identity=23.3870967741935, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=167, Percent_Identity=31.1377245508982, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34528; Mature: 34528

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVAVAEELNFTRAAERLHIAQPPLSRQIQQLEEEVGVLLFERGSRPLKLTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEECC
GRFFHAHARQLLAQTAELASMTQRVGQIERRLSIGFVASTLYGMLPKVIRRFRAEYPMVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTMHEMTTMDQIQALKDGRIDVGFGRIRYEDPNVRRILLRDERLIVALPSGHALLDGRTT
EHHHHHHHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCEEEEEECCCCEEECCCCH
ASLRDLVGDTLIIYPRTPRPSYADQVLALFHDRALEPSKIYEARELQIALGLVAAGEGVS
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEECCCCCE
VVPRSVAGLRREDVCYMELDDPQLVSPIIFSARLLDESEDIQAMLALTYRLYDEQKIPYY
ECCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEE
VPS
CCC
>Mature Secondary Structure
MELRHLRYFVAVAEELNFTRAAERLHIAQPPLSRQIQQLEEEVGVLLFERGSRPLKLTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEECC
GRFFHAHARQLLAQTAELASMTQRVGQIERRLSIGFVASTLYGMLPKVIRRFRAEYPMVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTMHEMTTMDQIQALKDGRIDVGFGRIRYEDPNVRRILLRDERLIVALPSGHALLDGRTT
EHHHHHHHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCEEEEEECCCCEEECCCCH
ASLRDLVGDTLIIYPRTPRPSYADQVLALFHDRALEPSKIYEARELQIALGLVAAGEGVS
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEECCCCCE
VVPRSVAGLRREDVCYMELDDPQLVSPIIFSARLLDESEDIQAMLALTYRLYDEQKIPYY
ECCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEE
VPS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]