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The map label for this gene is smpB

Identifier: 187928390

GI number: 187928390

Start: 1398494

End: 1398940

Strand: Reverse

Name: smpB

Synonym: Rpic_1301

Alternate gene names: 187928390

Gene position: 1398940-1398494 (Counterclockwise)

Preceding gene: 187928393

Following gene: 187928386

Centisome position: 35.48

GC content: 60.4

Gene sequence:

>447_bases
ATGACCATCGCCGACAACAAGAAGGCCTTTTTCGATTATTTCATCGAAGAGCGCTACGAGGCAGGGATGGCTCTCGAGGG
CTGGGAGGTGAAAGCCATCCGGGCCAACCGTGCGCAGATCAAGGAAGGTTACGTCGTCATTCGTAACGCCGAGCTGTTCC
TGATCGGGGCCCATATCAGCCCGCTGCAATCGGCCTCCACGCATGTCCATCCGGACCCCGTGCGCACCCGCAAACTGCTG
CTGCACGCCGAAGAGATCAAGAAGCTCATCGGCAAGGTCGAGCAGCGTGGCTACACGCTCGTTCCGCTCAACCTGCACTA
CACGCGCGGCCGCGTGAAGTGCGAAATCGGCCTCGCCAAGGGCAAGAAGCAGTTCGACAAGCGCGAAACCGAGAAGGACC
GCGACTGGCAGCGCGAAAAAGCCCGCCTCATGCGCGAGAAGGCTTGA

Upstream 100 bases:

>100_bases
GCGTCCCGGTTGGGATTGCGGCGGATTTGTTAAACTCGCCATTTTATCGCAGCGCATGCAGGGATCCTCACCCCAATGCG
CGCACCGCATATTCCAACGT

Downstream 100 bases:

>100_bases
GCCCTCCCGCTCCGAACTTTACGCGCTCGGCCGCTGCTGCTTCACGATCGTCAACGCTGACGCAATCATCGTGCTCAGGT
CGCCCATGTTCCCCGGCACG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTIADNKKAFFDYFIEERYEAGMALEGWEVKAIRANRAQIKEGYVVIRNAELFLIGAHISPLQSASTHVHPDPVRTRKLL
LHAEEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAKGKKQFDKRETEKDRDWQREKARLMREKA

Sequences:

>Translated_148_residues
MTIADNKKAFFDYFIEERYEAGMALEGWEVKAIRANRAQIKEGYVVIRNAELFLIGAHISPLQSASTHVHPDPVRTRKLL
LHAEEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAKGKKQFDKRETEKDRDWQREKARLMREKA
>Mature_147_residues
TIADNKKAFFDYFIEERYEAGMALEGWEVKAIRANRAQIKEGYVVIRNAELFLIGAHISPLQSASTHVHPDPVRTRKLLL
HAEEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAKGKKQFDKRETEKDRDWQREKARLMREKA

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=54.8611111111111, Blast_Score=167, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_RALPJ (B2UBC4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898877.1
- GeneID:   6286957
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1301
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17247; Mature: 17116

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIADNKKAFFDYFIEERYEAGMALEGWEVKAIRANRAQIKEGYVVIRNAELFLIGAHIS
CCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
PLQSASTHVHPDPVRTRKLLLHAEEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCC
GKKQFDKRETEKDRDWQREKARLMREKA
CHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIADNKKAFFDYFIEERYEAGMALEGWEVKAIRANRAQIKEGYVVIRNAELFLIGAHIS
CCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
PLQSASTHVHPDPVRTRKLLLHAEEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCC
GKKQFDKRETEKDRDWQREKARLMREKA
CHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA