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The map label for this gene is ppsA [H]

Identifier: 187928387

GI number: 187928387

Start: 1394521

End: 1396908

Strand: Direct

Name: ppsA [H]

Synonym: Rpic_1298

Alternate gene names: 187928387

Gene position: 1394521-1396908 (Clockwise)

Preceding gene: 187928385

Following gene: 187928388

Centisome position: 35.37

GC content: 63.65

Gene sequence:

>2388_bases
ATGACTAACCTGTCGCAAGACGGCGCCTATGTGCTGCCGTTCGAGCAATTGCGGATGACCGATGTCGAGGTCGTCGGCGG
TAAAAACGCTTCGCTGGGCGAGATGATCTCCCAGCTGGACGGCGCCGGCGTGCGCGTTCCGGGCGGTTTTGCCACCACGG
CACTGGCGTTCCGCGATTTCCTTGCACACAACAACCTGACCGAGCGCATCTCGCAGCGTCTGGCCAGCCTCGACGTCGAC
GACGTCAAGGCGCTGGCGGCCGCCGGCAAGGAGATCCGCGAATGGGTCGCCACCGCGCCGTTCCAGCCGCGTCTGGAGCA
GGAGATTCGCGAGTCGTACGCTCGCGTGTCCGCCCGCGAAGGCGCTGAGGCATCGTTTGCGGTGCGCTCGTCGGCCACGG
CGGAAGATCTGCCGGATGCTTCGTTCGCCGGCCAGCAAGAGAGCTATCTCAACGTCCTCGGCATCGACGACGTGCTCGAC
AAGATCAAGCACGTGTTCGCCTCGCTGTACAACGACCGTGCGATTTCGTACCGCGTGCACAAGGGTTTCGCCCATGACGT
GGTGGCCCTGTCTGCCGGCATCCAGCGTATGGTGCGCTCGGACTGCGGCGCCTCGGGCGTGATGTTCACCATCGACACCG
AATCGGGCTTCCAGGATGTCGTTTTCATCACCTCCAGCTACGGCCTGGGCGAAACGGTGGTGCAGGGCGCTGTGAACCCG
GACGAGTTCTACGTCTTCAAGCCTACGCTGGCCGCCGGCCAGTACCCCATCATCCGCCGCTCGATCGGTTCCAAGCTGAT
CAAGATGGAGTTCACCGCGCCGGGCGAAGCAGGCCGCGTGAAGACCGTCGACGTGCCGGGCGAGCTTCGCAACCGCTACT
CCATCAGCGATGCGGACGTGACGGAACTGGCCAAGTACGCCGTCATCATCGAGAAGCACTACGGCCGCCCGATGGATATC
GAGTGGGGCCGCGACGGCCGCGACGGCAAGATCTACATCCTGCAGGCCCGCCCCGAAACGGTGAAGAGCCAGGCACACGG
CAAGGTCGAGCAGCGTTTCCGCCTCAAGGGCTCAGCGCCGGTGCTGACCACCGGCCGCGCCATCGGCCAGAAGATCGGCA
CGGGCCCGGTGCGTGTGATCAACGACCCGGCTGAAATGGAACGCGTGCAGCCCGGCGACGTGCTGGTGGCCGACATGACC
GACCCGAACTGGGAGCCGGTGATGAAGCGCGCCTCAGCCATCGTCACCAACCGTGGCGGCCGTACCTGCCACGCGGCCAT
CATCGCGCGTGAGCTGGGTGTGCCGGCAGTCGTGGGCTGCGGCGATGCGACCGACCTGCTGAAGGACGGCACGCTGGTGA
CGGTCTCGTGCGCCGAAGGCGACGAAGGCAAGATCTACGACGGCCTGCTGGAAACCGAAATCACGGAAGTCCAGCGTGGC
GAAATGCCGCCGATCAGCACCAAGATCATGATGAACGTCGGCAATCCGCAGCTGGCGTTCGACTTCTGCCAGATTCCGAA
CGGTGGCGTGGGCTTGGCCCGCCTTGAGTTCATCATCAATAACAACATCGGCGTGCACCCGAAGGCGATTCTCGACTACC
CGCAAGTCGACAGCGACCTGAAGAAGGCTGTGGAAAGCGTGGCCCGCGGCCATGCCAGCCCGCGTGCGTTCTATGTCGAC
AAGCTGGCCGAGGGCATTGCCACCATCGCAGCGGCGTTCTATCCGAAACCCGTGATCGTGCGCCTGTCGGACTTCAAGTC
CAACGAGTACAAGAAGCTGATTGGCGGTTCCCGCTACGAGCCGGACGAAGAAAACCCGATGCTGGGTTTCCGTGGCGCCT
CGCGCTACATCGCCGAAGACTTTGCCGAAGCCTTCGAGATGGAGTGCCGTGCCATGAAGCGCGTGCGCGACGAGATGGGC
CTGACCAACGTCGAAGTGATGGTGCCGTTCGTGCGTACGCTCGGCCAGGCTGAGAAGGTTGTCGAGCTGCTGGCCAAGTA
CGGCCTGAAGCGCGGTGAGAACGGCCTGCGCCTGATCATGATGTGCGAAGTGCCGTCCAACGCGATCCTGGCCGAGCAAT
TCCTGCAGCATTTCGACGGTTTCTCGATCGGTTCGAACGACCTGACGCAGCTCACGCTGGGTCTGGACCGCGACTCCGGC
ATGGAGCTGCTCGCCCGCGACTTTGACGAGCGCGATCCGGCAGTCAAGTTCATGCTGCAGCGTGCCATTTCCACGGCGCT
GTCGATGAACAAGTACGTCGGCATCTGCGGTCAAGGCCCGTCCGACCATCCGGATTTTGCCGAGTGGCTGGCCAAGGAAG
GCATCTCGTCGATCTCGCTGAACCCGGACTCCGTGATCGACACGTGGCAGAAGCTGGGCTCGGCTTGA

Upstream 100 bases:

>100_bases
GGGCTTCCGGATGATTTAGCCAAGCGATTCGTACGGCCAGACGACAAGAAGAAAGCCCCGTGCGGATCCGACCGGTTTTT
CACTTAGTTTAGGGGCTTGT

Downstream 100 bases:

>100_bases
TTCCGACCTAGAGTGCCGTAAGACAAGGCGCGTATTCTGAAGGGTACGCGCCAACAAGAACGCGAAGTGCCCGACGGCGT
CCAACGCTGGCGGGGGCCGA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MTNLSQDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDGAGVRVPGGFATTALAFRDFLAHNNLTERISQRLASLDVD
DVKALAAAGKEIREWVATAPFQPRLEQEIRESYARVSAREGAEASFAVRSSATAEDLPDASFAGQQESYLNVLGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLAAGQYPIIRRSIGSKLIKMEFTAPGEAGRVKTVDVPGELRNRYSISDADVTELAKYAVIIEKHYGRPMDI
EWGRDGRDGKIYILQARPETVKSQAHGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVQRG
EMPPISTKIMMNVGNPQLAFDFCQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVD
KLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVRDEMG
LTNVEVMVPFVRTLGQAEKVVELLAKYGLKRGENGLRLIMMCEVPSNAILAEQFLQHFDGFSIGSNDLTQLTLGLDRDSG
MELLARDFDERDPAVKFMLQRAISTALSMNKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA

Sequences:

>Translated_795_residues
MTNLSQDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDGAGVRVPGGFATTALAFRDFLAHNNLTERISQRLASLDVD
DVKALAAAGKEIREWVATAPFQPRLEQEIRESYARVSAREGAEASFAVRSSATAEDLPDASFAGQQESYLNVLGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLAAGQYPIIRRSIGSKLIKMEFTAPGEAGRVKTVDVPGELRNRYSISDADVTELAKYAVIIEKHYGRPMDI
EWGRDGRDGKIYILQARPETVKSQAHGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVQRG
EMPPISTKIMMNVGNPQLAFDFCQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVD
KLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVRDEMG
LTNVEVMVPFVRTLGQAEKVVELLAKYGLKRGENGLRLIMMCEVPSNAILAEQFLQHFDGFSIGSNDLTQLTLGLDRDSG
MELLARDFDERDPAVKFMLQRAISTALSMNKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA
>Mature_794_residues
TNLSQDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDGAGVRVPGGFATTALAFRDFLAHNNLTERISQRLASLDVDD
VKALAAAGKEIREWVATAPFQPRLEQEIRESYARVSAREGAEASFAVRSSATAEDLPDASFAGQQESYLNVLGIDDVLDK
IKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNPD
EFYVFKPTLAAGQYPIIRRSIGSKLIKMEFTAPGEAGRVKTVDVPGELRNRYSISDADVTELAKYAVIIEKHYGRPMDIE
WGRDGRDGKIYILQARPETVKSQAHGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTD
PNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVQRGE
MPPISTKIMMNVGNPQLAFDFCQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVDK
LAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVRDEMGL
TNVEVMVPFVRTLGQAEKVVELLAKYGLKRGENGLRLIMMCEVPSNAILAEQFLQHFDGFSIGSNDLTQLTLGLDRDSGM
ELLARDFDERDPAVKFMLQRAISTALSMNKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=64.2676767676768, Blast_Score=1047, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=193, Percent_Identity=33.6787564766839, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788756, Length=409, Percent_Identity=24.4498777506112, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI48994992, Length=409, Percent_Identity=25.6723716381418, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789193, Length=390, Percent_Identity=26.1538461538462, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=336, Percent_Identity=28.2738095238095, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86787; Mature: 86656

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLSQDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDGAGVRVPGGFATTALAFRDF
CCCCCCCCCEEECHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHH
LAHNNLTERISQRLASLDVDDVKALAAAGKEIREWVATAPFQPRLEQEIRESYARVSARE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVLGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLAAGQYPIIRRSIGSKLIKMEFTAPGEAGRVKTVDVPGELRNRYSISDADV
CCEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCH
TELAKYAVIIEKHYGRPMDIEWGRDGRDGKIYILQARPETVKSQAHGKVEQRFRLKGSAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHEECCCCC
VLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
EEECCHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVQRG
CCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPPISTKIMMNVGNPQLAFDFCQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDL
CCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVRDEMGLTNVEVMVPFVRTLGQAEKV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHH
VELLAKYGLKRGENGLRLIMMCEVPSNAILAEQFLQHFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
MELLARDFDERDPAVKFMLQRAISTALSMNKYVGICGQGPSDHPDFAEWLAKEGISSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCEECC
NPDSVIDTWQKLGSA
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLSQDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDGAGVRVPGGFATTALAFRDF
CCCCCCCCEEECHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHH
LAHNNLTERISQRLASLDVDDVKALAAAGKEIREWVATAPFQPRLEQEIRESYARVSARE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVLGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLAAGQYPIIRRSIGSKLIKMEFTAPGEAGRVKTVDVPGELRNRYSISDADV
CCEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCH
TELAKYAVIIEKHYGRPMDIEWGRDGRDGKIYILQARPETVKSQAHGKVEQRFRLKGSAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHEECCCCC
VLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
EEECCHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVQRG
CCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPPISTKIMMNVGNPQLAFDFCQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDL
CCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVRDEMGLTNVEVMVPFVRTLGQAEKV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHH
VELLAKYGLKRGENGLRLIMMCEVPSNAILAEQFLQHFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
MELLARDFDERDPAVKFMLQRAISTALSMNKYVGICGQGPSDHPDFAEWLAKEGISSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCEECC
NPDSVIDTWQKLGSA
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]