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The map label for this gene is dapE

Identifier: 187928352

GI number: 187928352

Start: 1352275

End: 1353435

Strand: Reverse

Name: dapE

Synonym: Rpic_1263

Alternate gene names: 187928352

Gene position: 1353435-1352275 (Counterclockwise)

Preceding gene: 187928353

Following gene: 187928351

Centisome position: 34.33

GC content: 65.72

Gene sequence:

>1161_bases
ATGTCCCACATCCAGCCCACGCTTGCCCTCACTGAAGACCTGATCCGCCGCCGCTCCGTCACGCCCGAAGACAAGGGCTG
TCAGGACGTGCTGATCGAACGCCTGACCGCCGCCGGGTTCGAATGCGAAACCGTGATCAGCGGCCCGGACCACTTTCGGG
TAACTAACCTGTGGGCGGTCAAGCGCGGGCGCGCCGGCACCGACGGCAAGCTGCTCGTGTTCGCTGGCCACACCGATGTG
GTGCCCACCGGCCCCGTCGAGCAATGGCACTCCGATCCCTTCGAACCGACGCACCGTGACGGCAAGCTCTACGCGCGCGG
CGCGGCAGACATGAAGACGTCGATTGCCGGCTTCGTGGTGGCCTCGGAAGAATTTGTTGCCAAGCACCCGGACCACGCCG
GCTCGATCGGCTTTCTGATCACCAGCGACGAAGAAGGCCCGGCGCACGACGGCACCGTCAAGGTATGCGACCTGCTCCGC
GCACGCGGCGAGCGCCTCGATTACTGCGTGGTCGGTGAGCCGACTTCGGTGTCGACCCTCGGCGACATGGTCAAGAACGG
GCGACGCGGTTCGCTGTCGGGCAAGCTCACCGTCAACGGGGTACAGGGCCACATCGCCTATCCGCATCTGGCCAAGAATC
CGATCCACATGGCTGCCCCCGCGCTGGCCGAGCTGGCCGCCGCGAAGTGGGACGACGGCAATGCCTACTTCCCGCCGACC
ACGTGGCAGATGTCGAATATTCACGGCGGCACGGGCGCGACGAACATCATTCCCGGCCACGTCACGATCGACTTCAACTT
CCGCTTCTCGACCGCGAGCACGCCGGACGGCCTGAAGGCGCGCGTGCACAGCATTCTCGATGCGCACGGCCTCGACTACA
CGCTCGACTGGACGCTGGGCGGCGAGCCCTTCCTGACCGAGCGCGGCGAGCTGTCCGAAGCCCTCGCCTCCGCCATCCAG
GCCGAGTGCGGCGTGACCACCGAACTGTCGACCACGGGCGGTACCTCGGACGGCCGCTTCATCGCCAAGATCTGCCCGCA
GGTGATCGAGTTCGGCCCGCCGAACGCGAGCATCCACAAGATCGACGAGCACGTCGAAGTGGCGTTTATCGAGCCGCTGA
AAAACGTGTATCGCCGCGTCTTGGAAACGTTGATCGCCTGA

Upstream 100 bases:

>100_bases
CCGGTGCTCGTGCACGGCAAAAGCGTCAATGTCGGCTTCTCGGCGGATCAGTACGCGACCCTGTTCTAACCAAAACAACA
CATCACGGTGACTGTCTGTC

Downstream 100 bases:

>100_bases
GGTTGCCCCCGATGGCGCGCAACGTCGAGATCAAGGCACGTGTGCGCGATGTGGCTGCGCTCATTGAACGCGCGGCTGCC
ATCGCAGATTCCGGCCCCGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSHIQPTLALTEDLIRRRSVTPEDKGCQDVLIERLTAAGFECETVISGPDHFRVTNLWAVKRGRAGTDGKLLVFAGHTDV
VPTGPVEQWHSDPFEPTHRDGKLYARGAADMKTSIAGFVVASEEFVAKHPDHAGSIGFLITSDEEGPAHDGTVKVCDLLR
ARGERLDYCVVGEPTSVSTLGDMVKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHMAAPALAELAAAKWDDGNAYFPPT
TWQMSNIHGGTGATNIIPGHVTIDFNFRFSTASTPDGLKARVHSILDAHGLDYTLDWTLGGEPFLTERGELSEALASAIQ
AECGVTTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVAFIEPLKNVYRRVLETLIA

Sequences:

>Translated_386_residues
MSHIQPTLALTEDLIRRRSVTPEDKGCQDVLIERLTAAGFECETVISGPDHFRVTNLWAVKRGRAGTDGKLLVFAGHTDV
VPTGPVEQWHSDPFEPTHRDGKLYARGAADMKTSIAGFVVASEEFVAKHPDHAGSIGFLITSDEEGPAHDGTVKVCDLLR
ARGERLDYCVVGEPTSVSTLGDMVKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHMAAPALAELAAAKWDDGNAYFPPT
TWQMSNIHGGTGATNIIPGHVTIDFNFRFSTASTPDGLKARVHSILDAHGLDYTLDWTLGGEPFLTERGELSEALASAIQ
AECGVTTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVAFIEPLKNVYRRVLETLIA
>Mature_385_residues
SHIQPTLALTEDLIRRRSVTPEDKGCQDVLIERLTAAGFECETVISGPDHFRVTNLWAVKRGRAGTDGKLLVFAGHTDVV
PTGPVEQWHSDPFEPTHRDGKLYARGAADMKTSIAGFVVASEEFVAKHPDHAGSIGFLITSDEEGPAHDGTVKVCDLLRA
RGERLDYCVVGEPTSVSTLGDMVKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHMAAPALAELAAAKWDDGNAYFPPTT
WQMSNIHGGTGATNIIPGHVTIDFNFRFSTASTPDGLKARVHSILDAHGLDYTLDWTLGGEPFLTERGELSEALASAIQA
ECGVTTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVAFIEPLKNVYRRVLETLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=55.6430446194226, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790395, Length=322, Percent_Identity=25.4658385093168, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17551016, Length=264, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_RALPJ (B2UAZ1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898839.1
- ProteinModelPortal:   B2UAZ1
- MEROPS:   M20.010
- GeneID:   6288791
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1263
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- ProtClustDB:   PRK13009
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41588; Mature: 41457

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 79-79 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIQPTLALTEDLIRRRSVTPEDKGCQDVLIERLTAAGFECETVISGPDHFRVTNLWAV
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEE
KRGRAGTDGKLLVFAGHTDVVPTGPVEQWHSDPFEPTHRDGKLYARGAADMKTSIAGFVV
ECCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHEEEE
ASEEFVAKHPDHAGSIGFLITSDEEGPAHDGTVKVCDLLRARGERLDYCVVGEPTSVSTL
ECHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
GDMVKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHMAAPALAELAAAKWDDGNAYFPPT
HHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
TWQMSNIHGGTGATNIIPGHVTIDFNFRFSTASTPDGLKARVHSILDAHGLDYTLDWTLG
CEEECCCCCCCCCCCEECCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEC
GEPFLTERGELSEALASAIQAECGVTTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHK
CCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCHHHH
IDEHVEVAFIEPLKNVYRRVLETLIA
HCCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHIQPTLALTEDLIRRRSVTPEDKGCQDVLIERLTAAGFECETVISGPDHFRVTNLWAV
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEE
KRGRAGTDGKLLVFAGHTDVVPTGPVEQWHSDPFEPTHRDGKLYARGAADMKTSIAGFVV
ECCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHEEEE
ASEEFVAKHPDHAGSIGFLITSDEEGPAHDGTVKVCDLLRARGERLDYCVVGEPTSVSTL
ECHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
GDMVKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHMAAPALAELAAAKWDDGNAYFPPT
HHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
TWQMSNIHGGTGATNIIPGHVTIDFNFRFSTASTPDGLKARVHSILDAHGLDYTLDWTLG
CEEECCCCCCCCCCCEECCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEC
GEPFLTERGELSEALASAIQAECGVTTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHK
CCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCHHHH
IDEHVEVAFIEPLKNVYRRVLETLIA
HCCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA