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The map label for this gene is mls [H]

Identifier: 187928325

GI number: 187928325

Start: 1323699

End: 1325279

Strand: Direct

Name: mls [H]

Synonym: Rpic_1236

Alternate gene names: 187928325

Gene position: 1323699-1325279 (Clockwise)

Preceding gene: 187928324

Following gene: 187928326

Centisome position: 33.57

GC content: 62.43

Gene sequence:

>1581_bases
ATGGCGCTCACGCTGCCCCAAGGCATGGAGATCAAGGCTGAGATTCTGCCGGCCTATGAAGACATTCTGACCCCCGAAGC
GCTGGCGCTGGTCGCCAAGCTGCACCGTGCCTTCCAGCCGCGTCGTAAAGAGCTGCTGGCTGCCCGTGTCGAGCGCGCCA
AGCGCCTGGACGCCGGCGAGCGCCCGGATTTCCTGCCGGAAACCAAGGCCGTGCGTGAAGGCGACTGGAAGGTTGCTCCG
ATCCCGCCGGCACTGCATTGCCGCCGCGTGGAAATCACCGGCCCGGTCGATGCCAAGATGGTCATCAACGCGTTCAACTC
GGGCGCGGACAGCTACATGACCGACTTCGAAGATTCGAATTCGCCGAGCTGGCACAACCAGATCCAGGGCCAGGTCAACC
TGAAGGCTGCCATTCGCCGCACGCTCACGCTGGAGCAGAACGGCAAGACCTACAAGCTCAACGACAAGATCGCCACGCTG
CAGGTGCGTCCGCGCGGCTGGCATCTGGACGAGAAGCATGTGCTGATCGACGGCGAGCGCGTGTCCGGCGGCATCTTCGA
CTTTGCGCTGTTCCTCTTCCACAACGCAAAGGAGCAGATCGCGCGTGGCGCCGGCCCGTTCTTCTACCTGCCGAAGATGG
AAAGCCATCTGGAAGCGCGCCTTTGGAACGACATCTTCGTGATGGCACAGAACGAGATCGGCCTGCCGCAAGGCACGATC
AAGGCCACTGTGCTGATCGAAACCATCCTCGCCGCGTTCGAGATGGAAGAAATCCTGTACGAACTGCGTGAGCACAGCGC
AGGCCTGAATGCCGGCCGTTGGGACTACATCTTCTCGTGCATCAAGAAGTTCAAGGTCGACAAGAACTTCTGCCTGGCCG
ATCGCGCCAAGGTGACGATGACCTCGCCGTTCATGCGCGCCTACGCGCTGCTGCTGCTCAAGACCTGCCACAAGCGCGGT
GCGCCCGCCATCGGCGGCATGAGCGCGCTGATCCCGATCAAGAACGATCCGGAGAAGAACGCCATCGCCATGGCCGGCAT
CATCGGCGACAAGAAGCGCGATGCGACCGACGGCTACGATGGCGGCTGGGTGGCGCACCCGGGCCTGGTCGAGCCGGCCA
TGAAGGAATTCGTGGCAGTGCTGGGCGACAAGCCGAACCAGTTCGAGAAGCAACGCCCGGACGTGGAAGTGAAGGCTGCG
GACCTGCTGGACTTCCAGCCGGAAACGCCGATCACCGAAGCTGGCCTGCGCATGAACATCAACGTCGGCATCCACTACCT
GGGTGCCTGGCTGGCCGGCAACGGTTGCGTGCCGATCCACAACCTGATGGAAGACGCCGCCACGGCGGAAATCTCCCGCT
CGCAGGTGTGGCAGTGGATCCGCTCGCCCAAGGGCAAGCTGGAAGACGGCACCAAGGTCACGGCCGAACTGGTGCGCAAG
CTGATCCCGGAAGAATTGGCCAAGGTGAAGGAGACGGGCGCGGTGGGCCACTTTGACCGCGCTGCAGTGATCTTCGAACA
GATGTCGACGTCGGAAGACTTTGCGGAATTCCTGACCCTGCCGCTGTACGAAGAAATCTAA

Upstream 100 bases:

>100_bases
ACACAAGCGACGTAAGGCATTGCATTGAATCGGCCCAGTCATAGGGCCAAAGTAAGACCAAAGCGCGTTCAACCCTCACA
TCATCCTCTGGAGAACAACC

Downstream 100 bases:

>100_bases
AAAAAATCGCTCACAGTCCTACTGCGCGGCCCGATCTTGGCCCTGCGATGCTCGCCGTACTCATGTACGGCTGCGCTTCT
CGCGCCAACCTCGGGTCGCT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MALTLPQGMEIKAEILPAYEDILTPEALALVAKLHRAFQPRRKELLAARVERAKRLDAGERPDFLPETKAVREGDWKVAP
IPPALHCRRVEITGPVDAKMVINAFNSGADSYMTDFEDSNSPSWHNQIQGQVNLKAAIRRTLTLEQNGKTYKLNDKIATL
QVRPRGWHLDEKHVLIDGERVSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
KATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTMTSPFMRAYALLLLKTCHKRG
APAIGGMSALIPIKNDPEKNAIAMAGIIGDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVEVKAA
DLLDFQPETPITEAGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
LIPEELAKVKETGAVGHFDRAAVIFEQMSTSEDFAEFLTLPLYEEI

Sequences:

>Translated_526_residues
MALTLPQGMEIKAEILPAYEDILTPEALALVAKLHRAFQPRRKELLAARVERAKRLDAGERPDFLPETKAVREGDWKVAP
IPPALHCRRVEITGPVDAKMVINAFNSGADSYMTDFEDSNSPSWHNQIQGQVNLKAAIRRTLTLEQNGKTYKLNDKIATL
QVRPRGWHLDEKHVLIDGERVSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
KATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTMTSPFMRAYALLLLKTCHKRG
APAIGGMSALIPIKNDPEKNAIAMAGIIGDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVEVKAA
DLLDFQPETPITEAGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
LIPEELAKVKETGAVGHFDRAAVIFEQMSTSEDFAEFLTLPLYEEI
>Mature_525_residues
ALTLPQGMEIKAEILPAYEDILTPEALALVAKLHRAFQPRRKELLAARVERAKRLDAGERPDFLPETKAVREGDWKVAPI
PPALHCRRVEITGPVDAKMVINAFNSGADSYMTDFEDSNSPSWHNQIQGQVNLKAAIRRTLTLEQNGKTYKLNDKIATLQ
VRPRGWHLDEKHVLIDGERVSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTIK
ATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTMTSPFMRAYALLLLKTCHKRGA
PAIGGMSALIPIKNDPEKNAIAMAGIIGDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVEVKAAD
LLDFQPETPITEAGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRKL
IPEELAKVKETGAVGHFDRAAVIFEQMSTSEDFAEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=529, Percent_Identity=47.8260869565217, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=49.4274809160305, Blast_Score=500, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=50.7142857142857, Blast_Score=413, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324212, Length=515, Percent_Identity=51.0679611650485, Blast_Score=514, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6322222, Length=515, Percent_Identity=51.0679611650485, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 58719; Mature: 58588

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLPQGMEIKAEILPAYEDILTPEALALVAKLHRAFQPRRKELLAARVERAKRLDAGE
CEEECCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
RPDFLPETKAVREGDWKVAPIPPALHCRRVEITGPVDAKMVINAFNSGADSYMTDFEDSN
CCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCC
SPSWHNQIQGQVNLKAAIRRTLTLEQNGKTYKLNDKIATLQVRPRGWHLDEKHVLIDGER
CCCHHHHCCCCEEHHHHHHHHEEEECCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCC
VSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
CCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHH
KATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEE
TSPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMAGIIGDKKRDATDGYD
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCC
GGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVEVKAADLLDFQPETPITEAGLRMNI
CCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCEEEHHHHCCCCCCCCCCCCCEEEEE
NVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
EECCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LIPEELAKVKETGAVGHFDRAAVIFEQMSTSEDFAEFLTLPLYEEI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
ALTLPQGMEIKAEILPAYEDILTPEALALVAKLHRAFQPRRKELLAARVERAKRLDAGE
EEECCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
RPDFLPETKAVREGDWKVAPIPPALHCRRVEITGPVDAKMVINAFNSGADSYMTDFEDSN
CCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCC
SPSWHNQIQGQVNLKAAIRRTLTLEQNGKTYKLNDKIATLQVRPRGWHLDEKHVLIDGER
CCCHHHHCCCCEEHHHHHHHHEEEECCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCC
VSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
CCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHH
KATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEE
TSPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMAGIIGDKKRDATDGYD
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCC
GGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVEVKAADLLDFQPETPITEAGLRMNI
CCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCEEEHHHHCCCCCCCCCCCCCEEEEE
NVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
EECCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LIPEELAKVKETGAVGHFDRAAVIFEQMSTSEDFAEFLTLPLYEEI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA