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The map label for this gene is dmlR [H]

Identifier: 187928323

GI number: 187928323

Start: 1321767

End: 1322696

Strand: Reverse

Name: dmlR [H]

Synonym: Rpic_1234

Alternate gene names: 187928323

Gene position: 1322696-1321767 (Counterclockwise)

Preceding gene: 187928328

Following gene: 187928322

Centisome position: 33.55

GC content: 65.38

Gene sequence:

>930_bases
ATGGATCATTTCAAGCAGCTCGAAACCTTTGTCGCCGTCGCCACACGCGGCAGCTTGTCCGCCGCCGCCGCCGCGGAGGG
CGTGGCGCCTGCCATCATCGGGCGCCGCATCGACGCGCTGGAGGAGCGCCTGGGCGTAAAGCTGCTCGTGCGCACCACGC
GGCGCATCACCCTCACGTTCGAGGGATCGGCGTTCCTGGAAGATTGCCAACGTATTCTCAATGACCTGCACAACGCCGAG
GCCAGCGTCTCGGCCGGTGGCGTAAAAGCCAGCGGGCACCTGCGCGTGACCGCCCCGGCCGGTTTTGGCCGCCGCCATGT
CGCGCCGATGGTGCCGGACTTCATCGAGACGCATCCCGATGTCTCGATGACGCTGGACCTGGGCGACCGTGTCGTCGACC
TGGTCAACGAAGGCTTCGATTGCGCGATCCGCCTGGGCGACCTGCCCGATTCGAGCCTCGTTTCGATCCGCCTGTGGGAA
AACCGCCGCGTGGTGGTGGCTGCGCCGTCGTACCTGGAGCGCCGCGGCGTTCCAACGCAGGTGGAGCAGCTTTCGACGCA
CAACTGTCTGGCCTTTGGCGCCAGTGCCAACGTGCAGCGCGGCTGGGTCTTCCAGCAAGGCGGCAAGGCCGTCACCGTCA
AGGTGTCGGGCACGATGGAATGCACCGACGGCGCCGTGCTGCGCGAGTGGTGCCTGCAAGGCTATGGCCTGGCGTGGCGC
TCATGGTGGGAGGTCGGACACGACATTGCCACGGGCAAGCTGGTCACGGTGCTCGATGCCTTTGAAGCGCCGCCGATTGG
CATCCATGCGGTGTTTCCGCAGCGCAAGCATCTGCCGCTGCGCGTGCGTCTTTTTATCGATTTTCTAAAGAACACGTATG
GCAACCCGGCCTACTGGCGCCGGGCAGACGCCTTGATCGGGATGGATTAG

Upstream 100 bases:

>100_bases
TGGTCACTTGATCTTTAACTTTTACCAATGGAATGACGAGTGATAACCTTTATATTCGTCACAATCCCGCAGGCCCCGGT
TGCCACGGTATACATCAGGC

Downstream 100 bases:

>100_bases
CAATCACCCCGAGTGGGTGAAACGCCGCATGCGGCCTACAATCAAGACAAGAACATCTTCCCGCATACCACCTCAGGAGG
CCGCATGTTCCAGCACATCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAE
ASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFIETHPDVSMTLDLGDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLWE
NRRVVVAAPSYLERRGVPTQVEQLSTHNCLAFGASANVQRGWVFQQGGKAVTVKVSGTMECTDGAVLREWCLQGYGLAWR
SWWEVGHDIATGKLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLFIDFLKNTYGNPAYWRRADALIGMD

Sequences:

>Translated_309_residues
MDHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAE
ASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFIETHPDVSMTLDLGDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLWE
NRRVVVAAPSYLERRGVPTQVEQLSTHNCLAFGASANVQRGWVFQQGGKAVTVKVSGTMECTDGAVLREWCLQGYGLAWR
SWWEVGHDIATGKLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLFIDFLKNTYGNPAYWRRADALIGMD
>Mature_309_residues
MDHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTFEGSAFLEDCQRILNDLHNAE
ASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFIETHPDVSMTLDLGDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLWE
NRRVVVAAPSYLERRGVPTQVEQLSTHNCLAFGASANVQRGWVFQQGGKAVTVKVSGTMECTDGAVLREWCLQGYGLAWR
SWWEVGHDIATGKLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLFIDFLKNTYGNPAYWRRADALIGMD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=35.7388316151203, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=32.8671328671329, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=32.0819112627986, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.3448275862069, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.0102389078498, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=27.112676056338, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=27.4914089347079, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788706, Length=181, Percent_Identity=27.6243093922652, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=24.0802675585284, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=234, Percent_Identity=28.6324786324786, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=25.7028112449799, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=26.5734265734266, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33880; Mature: 33880

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTF
CCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFIETHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCC
VSMTLDLGDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLWENRRVVVAAPSYLERRGVPTQ
CEEEECCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHCCCCHH
VEQLSTHNCLAFGASANVQRGWVFQQGGKAVTVKVSGTMECTDGAVLREWCLQGYGLAWR
HHHHCCCCEEEECCCCCCCCCEEEECCCCEEEEEEECEEECCCCHHHHHHHHCCCCCHHH
SWWEVGHDIATGKLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLFIDFLKNTYGNPAYWR
HHHHHCCCCCCCHHEEEEHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RADALIGMD
HCCHHCCCC
>Mature Secondary Structure
MDHFKQLETFVAVATRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRRITLTF
CCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRVTAPAGFGRRHVAPMVPDFIETHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCC
VSMTLDLGDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLWENRRVVVAAPSYLERRGVPTQ
CEEEECCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHCCCCHH
VEQLSTHNCLAFGASANVQRGWVFQQGGKAVTVKVSGTMECTDGAVLREWCLQGYGLAWR
HHHHCCCCEEEECCCCCCCCCEEEECCCCEEEEEEECEEECCCCHHHHHHHHCCCCCHHH
SWWEVGHDIATGKLVTVLDAFEAPPIGIHAVFPQRKHLPLRVRLFIDFLKNTYGNPAYWR
HHHHHCCCCCCCHHEEEEHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RADALIGMD
HCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]