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The map label for this gene is lexA

Identifier: 187928268

GI number: 187928268

Start: 1268064

End: 1268714

Strand: Reverse

Name: lexA

Synonym: Rpic_1179

Alternate gene names: 187928268

Gene position: 1268714-1268064 (Counterclockwise)

Preceding gene: 187928278

Following gene: 187928267

Centisome position: 32.18

GC content: 64.21

Gene sequence:

>651_bases
ATGGCCACCCTCACCCCCCGCCAGCAGCAGATTTATGACCTCATCCGCCAGACGATCCAGCGCACGGGCTTTCCGCCGAC
GCGCGCCGAGATTGCCGCGGAGTTTGGCTTCTCGTCGCCCAATGCGGCTGAGGAACACCTCCGCGCGTTGGCACGCAAGG
GCGTGATTGAACTGACGCCGGGCGCCTCGCGTGGCATTCGGCTGCGGGCGGCGGGCGACACGGCGCAGCATCAGTTCTCG
CTGCCGTCGATGGGATTGATGCAGTTGACACTGCCGCTGGTGGGCCGCGTGGCCGCCGGTAGTCCGATCCTGGCTGCCGA
GCATATTGATCGCCAGTACCAAGTGGATCCGTCGCTGTTCTCTGCGCAGCCCGATTTTCTGCTGAAGGTGCGTGGTATGA
GCATGCGCGACGCCGGCATTCTCGATGGCGATCTACTGGCCGTGCAGCGCGCCAGTGAAGCCACCAACGGCAAGATCGTC
GTGGCTCGACTGGGCGACGACGTCACGGTCAAGCGCTTCCAGCGCAAGGGCCGGCATGTCGAGCTGATTGCCGAGAACCC
CGATTTCGAACCGATCCACGTTGATCTGGATCGCGATGAGTTTCACCTGGAGGGCTTGGCCGTCGGTCTGATCCGGCCTG
CTGCTCCGTAA

Upstream 100 bases:

>100_bases
TAAGGACTGGGCGCCTGCGCAACAGGTCACAACAAAATCACTTGCGCTTCCTGCGTCACTGTATAAAATCACAGCACCTG
TTCAAATATACAGTGCGCCG

Downstream 100 bases:

>100_bases
GTCTCCGGCGCATCTCAGCGCCAAACCTTCGCTGTTTGATTCCACTTGAAGGTGAGCCAGCGGCTCGCCGGGGCGGAGTC
TTGCCACTCGCTTTTCCACC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MATLTPRQQQIYDLIRQTIQRTGFPPTRAEIAAEFGFSSPNAAEEHLRALARKGVIELTPGASRGIRLRAAGDTAQHQFS
LPSMGLMQLTLPLVGRVAAGSPILAAEHIDRQYQVDPSLFSAQPDFLLKVRGMSMRDAGILDGDLLAVQRASEATNGKIV
VARLGDDVTVKRFQRKGRHVELIAENPDFEPIHVDLDRDEFHLEGLAVGLIRPAAP

Sequences:

>Translated_216_residues
MATLTPRQQQIYDLIRQTIQRTGFPPTRAEIAAEFGFSSPNAAEEHLRALARKGVIELTPGASRGIRLRAAGDTAQHQFS
LPSMGLMQLTLPLVGRVAAGSPILAAEHIDRQYQVDPSLFSAQPDFLLKVRGMSMRDAGILDGDLLAVQRASEATNGKIV
VARLGDDVTVKRFQRKGRHVELIAENPDFEPIHVDLDRDEFHLEGLAVGLIRPAAP
>Mature_215_residues
ATLTPRQQQIYDLIRQTIQRTGFPPTRAEIAAEFGFSSPNAAEEHLRALARKGVIELTPGASRGIRLRAAGDTAQHQFSL
PSMGLMQLTLPLVGRVAAGSPILAAEHIDRQYQVDPSLFSAQPDFLLKVRGMSMRDAGILDGDLLAVQRASEATNGKIVV
ARLGDDVTVKRFQRKGRHVELIAENPDFEPIHVDLDRDEFHLEGLAVGLIRPAAP

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=212, Percent_Identity=58.4905660377358, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_RALPJ (B2UAG1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898755.1
- MEROPS:   S24.001
- GeneID:   6289728
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1179
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23616; Mature: 23485

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: ACT_SITE 134-134 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTPRQQQIYDLIRQTIQRTGFPPTRAEIAAEFGFSSPNAAEEHLRALARKGVIELTP
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECC
GASRGIRLRAAGDTAQHQFSLPSMGLMQLTLPLVGRVAAGSPILAAEHIDRQYQVDPSLF
CCCCCEEEEECCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCEEEHHHCCCEEECCCHHH
SAQPDFLLKVRGMSMRDAGILDGDLLAVQRASEATNGKIVVARLGDDVTVKRFQRKGRHV
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEE
ELIAENPDFEPIHVDLDRDEFHLEGLAVGLIRPAAP
EEEECCCCCCEEEEECCCCCEEECEEEEEEECCCCC
>Mature Secondary Structure 
ATLTPRQQQIYDLIRQTIQRTGFPPTRAEIAAEFGFSSPNAAEEHLRALARKGVIELTP
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECC
GASRGIRLRAAGDTAQHQFSLPSMGLMQLTLPLVGRVAAGSPILAAEHIDRQYQVDPSLF
CCCCCEEEEECCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCEEEHHHCCCEEECCCHHH
SAQPDFLLKVRGMSMRDAGILDGDLLAVQRASEATNGKIVVARLGDDVTVKRFQRKGRHV
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEE
ELIAENPDFEPIHVDLDRDEFHLEGLAVGLIRPAAP
EEEECCCCCCEEEEECCCCCEEECEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA