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The map label for this gene is truB

Identifier: 187928212

GI number: 187928212

Start: 1195652

End: 1196608

Strand: Direct

Name: truB

Synonym: Rpic_1119

Alternate gene names: 187928212

Gene position: 1195652-1196608 (Clockwise)

Preceding gene: 187928211

Following gene: 187928213

Centisome position: 30.33

GC content: 64.58

Gene sequence:

>957_bases
ATGGCTGAGCACCAAGTGCCCAGGCCCCAAAAGCCGCCGCGGCGTGATGTGCATGGCGTGCTGCTCCTAGACAAGCCGAT
CGGCTGGTCGAGCAACGATGCGCTGATCCGCGCCAAACGCCTGCTGTGGGCCAAGAAGGCGGGGCATACCGGCACGCTCG
ATCCGCTTGCTACGGGACTGTTGCCCCTGTGTTTTGGCGAGGCGACCAAGTTTTCGCAGGATCTGCTCGATGCGGACAAG
ACGTACGAAACCGTCGTGCGCCTTGGGATCAGGACGAGTACCGCAGACGCGGAAGGCGAGGTGCTGAGCGAGCGCCCGGT
ATCGGTCACGCCCGAGCAACTGCAGGCAGCCATCGCGCGCTTCGTCGGCGAGATCGACCAGGTGCCGCCGATGCACTCGG
CGCTGAAGAAGGACGGCAAGCCGCTGTACGAGTACGCCCGTGCGGGGCAAACGGTGGAACGTGCTGCGCGTCGGGTAACG
ATCTACGCGATTGATGTGCTGGCAACCGACCTGCAATCGGCCGAACCAACGGTCACGTTGCGCGTGTCATGCAGTAAAGG
AACATACATCCGCACGTTGGGTGAAGACATTGGGGAGGCGCTGGGCTGCGGAGGGCATCTGGTTGCGCTGCGTCGCACCC
AGGTGGGCAACCTCACGCTGGACGGCGCCGTGACACTCGAAGCGTTGGACGCTGCTGCTGAAGATGCACGTGGTGCATTG
CTGGCGCCGGTCGATGCGCTGTTGCAGACGCTGCCGCGCGTTGAGCTGGATGCGCAAGAGAGCCGTCGCTTCCTGCATGG
CCAGCGCTTGCCGTTGCAATTGGCGTTGCCGAATGCAGACCAGGTGCGCGTCTACGGCGTGCGTGACGCGATCGCGGCGG
ATGCCACTGCGTCGCTGCTCGGAGTGGCTGCCTGGCAAGGCGGTGTGCTCCGGCCGGAGCGGCTCGTGCATCTTTAA

Upstream 100 bases:

>100_bases
CCGACACTGCATTTCCACTTCGACGGTTCGGTCGAGCGCGGTATCGAGATGTCGCGCCTGATCGATGAGGCCAACGCCAC
GCGTGCCAAAGACGATTGAG

Downstream 100 bases:

>100_bases
GCCGATCGGCTGACCAAAAAAGCCACCGAACTTCGGTGGCTTTTTTCATGGAGCTGACCCGTCCTGAATAAGGTTGACAC
TTTTTCCCCGCCAGGGAGTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAEHQVPRPQKPPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYETVVRLGIRTSTADAEGEVLSERPVSVTPEQLQAAIARFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVT
IYAIDVLATDLQSAEPTVTLRVSCSKGTYIRTLGEDIGEALGCGGHLVALRRTQVGNLTLDGAVTLEALDAAAEDARGAL
LAPVDALLQTLPRVELDAQESRRFLHGQRLPLQLALPNADQVRVYGVRDAIAADATASLLGVAAWQGGVLRPERLVHL

Sequences:

>Translated_318_residues
MAEHQVPRPQKPPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYETVVRLGIRTSTADAEGEVLSERPVSVTPEQLQAAIARFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVT
IYAIDVLATDLQSAEPTVTLRVSCSKGTYIRTLGEDIGEALGCGGHLVALRRTQVGNLTLDGAVTLEALDAAAEDARGAL
LAPVDALLQTLPRVELDAQESRRFLHGQRLPLQLALPNADQVRVYGVRDAIAADATASLLGVAAWQGGVLRPERLVHL
>Mature_317_residues
AEHQVPRPQKPPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADKT
YETVVRLGIRTSTADAEGEVLSERPVSVTPEQLQAAIARFVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTI
YAIDVLATDLQSAEPTVTLRVSCSKGTYIRTLGEDIGEALGCGGHLVALRRTQVGNLTLDGAVTLEALDAAAEDARGALL
APVDALLQTLPRVELDAQESRRFLHGQRLPLQLALPNADQVRVYGVRDAIAADATASLLGVAAWQGGVLRPERLVHL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=37.9464285714286, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503337, Length=303, Percent_Identity=28.0528052805281, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI215599015, Length=303, Percent_Identity=28.0528052805281, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI2367200, Length=264, Percent_Identity=50, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17553978, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=131, Percent_Identity=43.5114503816794, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=298, Percent_Identity=29.1946308724832, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364189, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364187, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364185, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364183, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI62471759, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17975520, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RALPJ (B2UAA5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898699.1
- GeneID:   6286981
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1119
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01851
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34282; Mature: 34150

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHQVPRPQKPPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYETVVRLGIRTSTADAEGEVLSERPVSVTPEQLQAAIAR
HHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHH
FVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTIYAIDVLATDLQSAEPTVTL
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHEEEEEEHHHHHHHHCCCCCEEEE
RVSCSKGTYIRTLGEDIGEALGCGGHLVALRRTQVGNLTLDGAVTLEALDAAAEDARGAL
EEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCEEHHHHHHHHHHHHCCCC
LAPVDALLQTLPRVELDAQESRRFLHGQRLPLQLALPNADQVRVYGVRDAIAADATASLL
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHH
GVAAWQGGVLRPERLVHL
HHHHHCCCCCCHHHHCCC
>Mature Secondary Structure 
AEHQVPRPQKPPRRDVHGVLLLDKPIGWSSNDALIRAKRLLWAKKAGHTGTLDPLATGL
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYETVVRLGIRTSTADAEGEVLSERPVSVTPEQLQAAIAR
HHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHH
FVGEIDQVPPMHSALKKDGKPLYEYARAGQTVERAARRVTIYAIDVLATDLQSAEPTVTL
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHEEEEEEHHHHHHHHCCCCCEEEE
RVSCSKGTYIRTLGEDIGEALGCGGHLVALRRTQVGNLTLDGAVTLEALDAAAEDARGAL
EEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCEEHHHHHHHHHHHHCCCC
LAPVDALLQTLPRVELDAQESRRFLHGQRLPLQLALPNADQVRVYGVRDAIAADATASLL
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHH
GVAAWQGGVLRPERLVHL
HHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA