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The map label for this gene is rlmN

Identifier: 187928149

GI number: 187928149

Start: 1121969

End: 1123120

Strand: Direct

Name: rlmN

Synonym: Rpic_1056

Alternate gene names: 187928149

Gene position: 1121969-1123120 (Clockwise)

Preceding gene: 187928148

Following gene: 187928150

Centisome position: 28.46

GC content: 62.76

Gene sequence:

>1152_bases
ATGAGCGATGTGGTGAATCTCCTCGATTTCGACGCACAGGGTCTTCTCGCGTACTGCGAGAGCCTGGGTGAGAAGTCGTT
CCGCGCCAAGCAATTGCAGCGCTGGATTCACCAGTCGGGTGCGTCCGAGTTTGGCGAGATGACGGATCTCGCCAAGTCGC
TGCGCGAAAAGCTCGCGACCCGCGCCAACATCCAGGCGCCGGCGGTCATTTCCGATCACCTTTCGTCGGACGGCACACGC
AAGTGGCTGGTCGACGTTGGGCAGGGCAATGCCGTCGAGACGGTCTACATCCCCGAAGAGACGCGCGGCACTCTATGTGT
GTCGTCTCAGGCGGGGTGTGCCGTCAACTGCCGGTTCTGTTCGACCGGCAAGCAAGGTTTCTCGCGCAATCTCAGCACTG
GCGAGATCATCGGCCAGCTCTGGATGGCCGAATTCGCCATGCGTAAGCAGCTTGGCCGTGGCCCCAAGGACGACCGCGTT
ATCACCAACGTGGTGATGATGGGCATGGGTGAGCCGCTGCTGAACTACGACGCTGTGGTGCCGGCTCTGGCCCTGATGCT
TGACGACAACGCGTATGGCCTTTCGCGCCGGCGCGTAACGGTATCCACGTCCGGCGTGGTTCCGATGATGGACCGCCTTG
CGCGGGATGTGCCGGTGGCGCTTGCTGTGTCGCTGCATGCGTCCAACGATGCGCTGCGCGACGTGCTCGTGCCGCTCAAC
AAGAAGTACCCGCTGGCCGAACTGATGGCTGCCTGCCGCCGCTATCTCGAGTTTGCCCCGCGTGATTTCATCACCTTTGA
ATACTGCATGCTCGACGGCGTCAACGACACCGTCGAGCATGCCCGTGAACTGCTGCGCGTCGTGGCCGACGTGCCGTGCA
AGTTCAACCTGATCCCATTCAACCCGTTCCCGGAATCGGGCTTGAAGCGCTCGAACAATGAGCAGATCCGCCGCTTCTCG
CAAGTGCTGCTGGATGCCGGCATCGTCACCACCATCCGCAAGACGCGGGGCGACGACATCGATGCGGCCTGCGGCCAGCT
CGCTGGCGAGGTCAAGGACCGCACGCGCTTGGCCGAGCGCGGCAAGTTCGGCAAGATCGTCGAGATCCCCGTGGTCGGGG
CCGACTCAACTCACCGCATGGGTACCGCATGA

Upstream 100 bases:

>100_bases
ACGGCTGCCGTGGAAGTCGCCTTCTTCTTCCCGGGCCTGAACATTTACAGCCGCTAAGCGTCAACACTAGACTGGGATTG
GTGGTTTCGGGTTGCAAGTC

Downstream 100 bases:

>100_bases
AGCGTTGGTCGACTGCTGTCAGTCTGGTCTTCACGCTGGCAGCCGCCGGGTGCGCGTTGTCGCCGCCGGTCCAGACACAG
ACACGAGAAGTTACAACAGT

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSDVVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGASEFGEMTDLAKSLREKLATRANIQAPAVISDHLSSDGTR
KWLVDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRKQLGRGPKDDRV
ITNVVMMGMGEPLLNYDAVVPALALMLDDNAYGLSRRRVTVSTSGVVPMMDRLARDVPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDTVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFS
QVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKIVEIPVVGADSTHRMGTA

Sequences:

>Translated_383_residues
MSDVVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGASEFGEMTDLAKSLREKLATRANIQAPAVISDHLSSDGTR
KWLVDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRKQLGRGPKDDRV
ITNVVMMGMGEPLLNYDAVVPALALMLDDNAYGLSRRRVTVSTSGVVPMMDRLARDVPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDTVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFS
QVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKIVEIPVVGADSTHRMGTA
>Mature_382_residues
SDVVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGASEFGEMTDLAKSLREKLATRANIQAPAVISDHLSSDGTRK
WLVDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRKQLGRGPKDDRVI
TNVVMMGMGEPLLNYDAVVPALALMLDDNAYGLSRRRVTVSTSGVVPMMDRLARDVPVALAVSLHASNDALRDVLVPLNK
KYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDTVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFSQ
VLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKIVEIPVVGADSTHRMGTA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=373, Percent_Identity=54.6916890080429, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_RALPJ (B2U9U6)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898636.1
- ProteinModelPortal:   B2U9U6
- GeneID:   6286055
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1056
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK896874
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42148; Mature: 42017

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGASEFGEMTDLAKSLREKLAT
CCCHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RANIQAPAVISDHLSSDGTRKWLVDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HCCCCCCHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIIGQLWMAEFAMRKQLGRGPKDDRVITNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
PALALMLDDNAYGLSRRRVTVSTSGVVPMMDRLARDVPVALAVSLHASNDALRDVLVPLN
HHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDTVEHARELLRVVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFSQVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKIVEIPVVGADSTHRMGTA
CCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDVVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGASEFGEMTDLAKSLREKLAT
CCHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RANIQAPAVISDHLSSDGTRKWLVDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HCCCCCCHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIIGQLWMAEFAMRKQLGRGPKDDRVITNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
PALALMLDDNAYGLSRRRVTVSTSGVVPMMDRLARDVPVALAVSLHASNDALRDVLVPLN
HHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDTVEHARELLRVVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFSQVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKIVEIPVVGADSTHRMGTA
CCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA