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The map label for this gene is phoB [H]

Identifier: 187928038

GI number: 187928038

Start: 999044

End: 999751

Strand: Direct

Name: phoB [H]

Synonym: Rpic_0943

Alternate gene names: 187928038

Gene position: 999044-999751 (Clockwise)

Preceding gene: 187928037

Following gene: 187928039

Centisome position: 25.34

GC content: 63.84

Gene sequence:

>708_bases
ATGAAAATCGGTCTGATCGATACGCACCTCGCCCGCCAGCAGGCGATCCACTCCGCGTTGCAGCCCACGCGCTTTGAATG
TGTGCCGCTGTTTCTCTCTCGCCAGGTCTTTGAAGCCATCGATCTGCACGGCATCAACCTGCTGATGGTCGGCGCGCATG
CGCACGACATGCCAGGCCTGTCGCTGGTGCGCAGCGTGCGCGAGCGCGTGGGCCCCGGTATGGCGATCGTCTACCTCGCC
GACGGCCAGGCCGATACCGAAACCGCAGAGGCGCTCAACCACGGTGCAGACCTGTGCTTCCAAGGTGATATCCGTGCACC
TGAGTTGCTGGCGCGGCTCGATGCGTTGATCCGCCGCTTTGCCGTGAGCCGCGCCGTGCGGGCGTCGGAGATTCGCCTGG
GGCCGTATACGCTCGATCGGCAGAATCGTTCCATCCGCCTGCACGATGCGTCGGTGTCAGTGCATGCGCGTGAGTTTGAG
TTGGCGCTGCTCTTGTTTGCGAATGCGGGGCGTGTACTCTCGCGCGCAGACATCGAACTGGCGCTCTGGGGCCGCGAGCT
GAGCCCGTATTCACGCACGCTCGATACGCATGTCTCGCGCCTGCGCAAGAAATTGCTGCTCGGCACGGAAAACGGTCTGC
GGCTGCGCGCCATCTACGGGCAGGGTTTCTGCCTGGAGCGCGTGATCGACGCCGAAGCAACGCACTGA

Upstream 100 bases:

>100_bases
TCCATGTGGCGCGGCGGTGGTCGCGACGGCAGCAATGCACAACAACATCGTCGCACCACCGCCTGATTGCGCCGCACGCA
GTCAACTACGAAACCGAGCC

Downstream 100 bases:

>100_bases
CAGCCTTGACCCGCCGGAGCCGCTGCCATGCAATTGCTCTGTATCACCATCCTCATCGGCTTTCTGGCCGGGCTTGTGGC
CCGATGGGTGACGCCGCGCT

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKIGLIDTHLARQQAIHSALQPTRFECVPLFLSRQVFEAIDLHGINLLMVGAHAHDMPGLSLVRSVRERVGPGMAIVYLA
DGQADTETAEALNHGADLCFQGDIRAPELLARLDALIRRFAVSRAVRASEIRLGPYTLDRQNRSIRLHDASVSVHAREFE
LALLLFANAGRVLSRADIELALWGRELSPYSRTLDTHVSRLRKKLLLGTENGLRLRAIYGQGFCLERVIDAEATH

Sequences:

>Translated_235_residues
MKIGLIDTHLARQQAIHSALQPTRFECVPLFLSRQVFEAIDLHGINLLMVGAHAHDMPGLSLVRSVRERVGPGMAIVYLA
DGQADTETAEALNHGADLCFQGDIRAPELLARLDALIRRFAVSRAVRASEIRLGPYTLDRQNRSIRLHDASVSVHAREFE
LALLLFANAGRVLSRADIELALWGRELSPYSRTLDTHVSRLRKKLLLGTENGLRLRAIYGQGFCLERVIDAEATH
>Mature_235_residues
MKIGLIDTHLARQQAIHSALQPTRFECVPLFLSRQVFEAIDLHGINLLMVGAHAHDMPGLSLVRSVRERVGPGMAIVYLA
DGQADTETAEALNHGADLCFQGDIRAPELLARLDALIRRFAVSRAVRASEIRLGPYTLDRQNRSIRLHDASVSVHAREFE
LALLLFANAGRVLSRADIELALWGRELSPYSRTLDTHVSRLRKKLLLGTENGLRLRAIYGQGFCLERVIDAEATH

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=30.2222222222222, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787375, Length=171, Percent_Identity=28.0701754385965, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=24.7826086956522, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26143; Mature: 26143

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGLIDTHLARQQAIHSALQPTRFECVPLFLSRQVFEAIDLHGINLLMVGAHAHDMPGL
CEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
SLVRSVRERVGPGMAIVYLADGQADTETAEALNHGADLCFQGDIRAPELLARLDALIRRF
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
AVSRAVRASEIRLGPYTLDRQNRSIRLHDASVSVHAREFELALLLFANAGRVLSRADIEL
HHHHHHHHHHEEECCEEECCCCCEEEEEECEEEEEHHHHEEEEEEEECCCCCHHHCCEEE
ALWGRELSPYSRTLDTHVSRLRKKLLLGTENGLRLRAIYGQGFCLERVIDAEATH
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKIGLIDTHLARQQAIHSALQPTRFECVPLFLSRQVFEAIDLHGINLLMVGAHAHDMPGL
CEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
SLVRSVRERVGPGMAIVYLADGQADTETAEALNHGADLCFQGDIRAPELLARLDALIRRF
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
AVSRAVRASEIRLGPYTLDRQNRSIRLHDASVSVHAREFELALLLFANAGRVLSRADIEL
HHHHHHHHHHEEECCEEECCCCCEEEEEECEEEEEHHHHEEEEEEEECCCCCHHHCCEEE
ALWGRELSPYSRTLDTHVSRLRKKLLLGTENGLRLRAIYGQGFCLERVIDAEATH
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]