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The map label for this gene is rbsB [H]

Identifier: 187927977

GI number: 187927977

Start: 936718

End: 937677

Strand: Reverse

Name: rbsB [H]

Synonym: Rpic_0882

Alternate gene names: 187927977

Gene position: 937677-936718 (Counterclockwise)

Preceding gene: 187927987

Following gene: 187927976

Centisome position: 23.78

GC content: 63.85

Gene sequence:

>960_bases
ATGGATATCCGCCGTCGCCGCGCCCTGCAACTTGGCGCGGGCTCTGTCGTAGCTGCTGCTCTGCCGTTCCCATTTTCGCT
GGCGCACGCCGCCAACCAGCCGCCCAAGGTGGCGTTGGTGATGAAGTCGCTTGCCAACGAGTTCTTCCTGACCATGGAGA
ACGGCGCGCGTAAGCATCAGAAGGATCACGCGTCGGAGTATTCACTGCTGACCAACGGCATTCGCGACGAAACCGACACC
GCTGGCCAAATCCGTCTGGTCGAGCAGATGATCGTCGCGCGCGTGGATGCGCTGGTGCTGGCCCCGGCCGATTCCAAGGC
GTTGGTGCCGGTCGTCAAGAAGGCCGTGGACGCGGGCATTCTCGTCATCAACATCGACAACAGGCTGGACCCCGCGGCAC
TGAAAGAGAAAGGGCTGAACGTGCCCTTCGTGGGGCCGGACAACCGCAAGGGTGCACGACTGGTGGGCGACTTCGTTGCC
AAGTCGCTCAAGCCGGGCGATGCCGTGGGCATCATCGAAGGCATACCGACCACCACGAACGCCCAGCAACGTACCGCTGG
CTTCAAGGACGCCGCCGAAGCCGCCAAGCTGAAGATCGCGGGGGTGCAATCGGGCGAATGGGAAATCGACAAGGGAAACA
AGGTGGCAGCCGCCATGCTGCGCTCCGAGCCCGACCTGAAGGCCCTGCTGTGCGGCAACGACAACATGGCGATCGGCGCC
GTATCGGCTGTGCGCGCGGCGGGCAAGCTGGGCAAGGTGCTGATTGGCGGCTATGACAACATCGACGCCATCAAGGCCAT
GCTGGCTGACGGGCGTGTCGTTGCGACGGCCGATCAGCACGCCGATCAGCAAGCCGTCTACGGCATCGAGACCGCCCTGA
AGGCGTTAGCCGCCAAGACGCCGCAAGCGAAGCTGTCGGGTGTCGTCGAGACACCGGTCAACCTCGTCACGCGCACGTAA

Upstream 100 bases:

>100_bases
GAGCGAGGCGCGATTGTCCTTAGATATGCCTGACGGCATGAGGCAGCGACGACGCCCGCACCCACCCGCCCCAGATAGGC
CTCTTCCTGGAGGAGACACC

Downstream 100 bases:

>100_bases
CGCCACAGACCGGCAAGCCGCCGCCAGCCATGTCTGCCGCTTACGAACCGCGCCCGCCGCTGCTTCGTGTTTCCGCCGTC
AGCAAGCACTACGCCGCCCC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDIRRRRALQLGAGSVVAAALPFPFSLAHAANQPPKVALVMKSLANEFFLTMENGARKHQKDHASEYSLLTNGIRDETDT
AGQIRLVEQMIVARVDALVLAPADSKALVPVVKKAVDAGILVINIDNRLDPAALKEKGLNVPFVGPDNRKGARLVGDFVA
KSLKPGDAVGIIEGIPTTTNAQQRTAGFKDAAEAAKLKIAGVQSGEWEIDKGNKVAAAMLRSEPDLKALLCGNDNMAIGA
VSAVRAAGKLGKVLIGGYDNIDAIKAMLADGRVVATADQHADQQAVYGIETALKALAAKTPQAKLSGVVETPVNLVTRT

Sequences:

>Translated_319_residues
MDIRRRRALQLGAGSVVAAALPFPFSLAHAANQPPKVALVMKSLANEFFLTMENGARKHQKDHASEYSLLTNGIRDETDT
AGQIRLVEQMIVARVDALVLAPADSKALVPVVKKAVDAGILVINIDNRLDPAALKEKGLNVPFVGPDNRKGARLVGDFVA
KSLKPGDAVGIIEGIPTTTNAQQRTAGFKDAAEAAKLKIAGVQSGEWEIDKGNKVAAAMLRSEPDLKALLCGNDNMAIGA
VSAVRAAGKLGKVLIGGYDNIDAIKAMLADGRVVATADQHADQQAVYGIETALKALAAKTPQAKLSGVVETPVNLVTRT
>Mature_319_residues
MDIRRRRALQLGAGSVVAAALPFPFSLAHAANQPPKVALVMKSLANEFFLTMENGARKHQKDHASEYSLLTNGIRDETDT
AGQIRLVEQMIVARVDALVLAPADSKALVPVVKKAVDAGILVINIDNRLDPAALKEKGLNVPFVGPDNRKGARLVGDFVA
KSLKPGDAVGIIEGIPTTTNAQQRTAGFKDAAEAAKLKIAGVQSGEWEIDKGNKVAAAMLRSEPDLKALLCGNDNMAIGA
VSAVRAAGKLGKVLIGGYDNIDAIKAMLADGRVVATADQHADQQAVYGIETALKALAAKTPQAKLSGVVETPVNLVTRT

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=279, Percent_Identity=32.258064516129, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790526, Length=260, Percent_Identity=32.6923076923077, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790674, Length=204, Percent_Identity=32.843137254902, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33489; Mature: 33489

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRRRRALQLGAGSVVAAALPFPFSLAHAANQPPKVALVMKSLANEFFLTMENGARKHQ
CCCCHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHH
KDHASEYSLLTNGIRDETDTAGQIRLVEQMIVARVDALVLAPADSKALVPVVKKAVDAGI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCE
LVINIDNRLDPAALKEKGLNVPFVGPDNRKGARLVGDFVAKSLKPGDAVGIIEGIPTTTN
EEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
AQQRTAGFKDAAEAAKLKIAGVQSGEWEIDKGNKVAAAMLRSEPDLKALLCGNDNMAIGA
CHHHHCCCHHHHHHHEEEEEECCCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHH
VSAVRAAGKLGKVLIGGYDNIDAIKAMLADGRVVATADQHADQQAVYGIETALKALAAKT
HHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
PQAKLSGVVETPVNLVTRT
CHHHHCCHHHCCHHHHCCC
>Mature Secondary Structure
MDIRRRRALQLGAGSVVAAALPFPFSLAHAANQPPKVALVMKSLANEFFLTMENGARKHQ
CCCCHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHH
KDHASEYSLLTNGIRDETDTAGQIRLVEQMIVARVDALVLAPADSKALVPVVKKAVDAGI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCE
LVINIDNRLDPAALKEKGLNVPFVGPDNRKGARLVGDFVAKSLKPGDAVGIIEGIPTTTN
EEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
AQQRTAGFKDAAEAAKLKIAGVQSGEWEIDKGNKVAAAMLRSEPDLKALLCGNDNMAIGA
CHHHHCCCHHHHHHHEEEEEECCCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHH
VSAVRAAGKLGKVLIGGYDNIDAIKAMLADGRVVATADQHADQQAVYGIETALKALAAKT
HHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
PQAKLSGVVETPVNLVTRT
CHHHHCCHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]