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The map label for this gene is mltB [H]

Identifier: 187927884

GI number: 187927884

Start: 834117

End: 835241

Strand: Reverse

Name: mltB [H]

Synonym: Rpic_0788

Alternate gene names: 187927884

Gene position: 835241-834117 (Counterclockwise)

Preceding gene: 187927892

Following gene: 187927866

Centisome position: 21.19

GC content: 65.33

Gene sequence:

>1125_bases
ATGAGCCAATCGCCTTCTTCCCGACGTCCCGTATTGCGCGCCCTGGTGGCTGGCGCAGCGCTCTCCGCGCTCTCTTTCCC
GGCGCTCTCGCTGGCTGCCAAGAAGACCGCCAAATCGCCCAAGCGCCGCGTCGCGGTGCATGAAGAAGAGATCGACCCCG
ATCGTTATCGCAACAACCCGCAAACGCAGGCCTTCATCGGCATGCTGGTCGCGCAGTACAACTTTGACCGGACATCGCTC
GAAACGCTGTTTGCGGGCACGGCCTATTCCGCCACGGTGGCCCGCCTGATCCTGCCGCCGGCCACGCCCGCGCGTCGCAG
CTGGACGGCCTATCGCGGCCGCTTCATCGAGCCGATCCGCATCGGCGCCGGCGTGCGGTTCTGGCAGCAATACGGCGACA
CACTGCGCCGCGCCGAGAACGAATTCGGCGTGCCGGCCGATGTGATCGTGGGCATCCTGGGCGTGGAGACCATCTACGGC
CGCGACATGGGCAACTTCCGCGTAATGGACGCGCTGTCCACGCTGGCCTTCGACTACCCCGACACGCCCAACCGCGAAGA
CCGCAGCACGATGTTCCGCAACCAGCTCAAGGACTTCCTGCTCTGGTGCCGTGACACGGGCACGGAGACGTTCTCGGTCC
TGGGCTCGTACGCGGGCGCAGTCGGTATTCCGCAATTCATGCCGACCAGCATCCGCGAATACGCCATCGATTACGACCAC
GACGGCCACATCGACCTGCGCAACAGCGCGGTGGATGCCATCGGCAGCGTTGCCCGCTTCCTGCAGATGCACGGCTGGGA
GCCAAACCGCCCGGTGATGTGGAACATTGCCGGCGATGCCGACAGCCAGGGCATTGCGGCCGCCGCCGCAGATGGCCAGC
CGTATCCCGGCATGACGTTGTCCAGGCTCACACGCGCTGGTCTGGCATTGGCTCCGGGTGTGGATACCGCGCGCGAGCAA
GAAACCGAAGTGCTGATGATCGATCTGCCCACGCCCGGCCAACCGACGGAATACCGCGTGGGGCTGCGCAACTTCTACGT
GCTCACACGCTATAACCGCAGCTTCTTCTATGCCGCCGCGGTGTACGAGTTGGGCCAGGCCGTGCGGCAGGCGATGCAGG
GTTGA

Upstream 100 bases:

>100_bases
CAGCCGCTACGTTAGCACGGCTGTGCGACAACGCCGCGTGCCCTGGACGATCCAACAGACGCATCCGCTACAATGCCCCG
ATCCCGTATGAAGCCTCCCC

Downstream 100 bases:

>100_bases
CCCGCCGTCCACACAAACAAAAAGCGCAGCCGAAGCTGCGCTTTTTTCATGGCGATGCGGCGAGCCGTCAGGCGGGGAAG
ACCCCCGTCGACAGGTAACG

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSQSPSSRRPVLRALVAGAALSALSFPALSLAAKKTAKSPKRRVAVHEEEIDPDRYRNNPQTQAFIGMLVAQYNFDRTSL
ETLFAGTAYSATVARLILPPATPARRSWTAYRGRFIEPIRIGAGVRFWQQYGDTLRRAENEFGVPADVIVGILGVETIYG
RDMGNFRVMDALSTLAFDYPDTPNREDRSTMFRNQLKDFLLWCRDTGTETFSVLGSYAGAVGIPQFMPTSIREYAIDYDH
DGHIDLRNSAVDAIGSVARFLQMHGWEPNRPVMWNIAGDADSQGIAAAAADGQPYPGMTLSRLTRAGLALAPGVDTAREQ
ETEVLMIDLPTPGQPTEYRVGLRNFYVLTRYNRSFFYAAAVYELGQAVRQAMQG

Sequences:

>Translated_374_residues
MSQSPSSRRPVLRALVAGAALSALSFPALSLAAKKTAKSPKRRVAVHEEEIDPDRYRNNPQTQAFIGMLVAQYNFDRTSL
ETLFAGTAYSATVARLILPPATPARRSWTAYRGRFIEPIRIGAGVRFWQQYGDTLRRAENEFGVPADVIVGILGVETIYG
RDMGNFRVMDALSTLAFDYPDTPNREDRSTMFRNQLKDFLLWCRDTGTETFSVLGSYAGAVGIPQFMPTSIREYAIDYDH
DGHIDLRNSAVDAIGSVARFLQMHGWEPNRPVMWNIAGDADSQGIAAAAADGQPYPGMTLSRLTRAGLALAPGVDTAREQ
ETEVLMIDLPTPGQPTEYRVGLRNFYVLTRYNRSFFYAAAVYELGQAVRQAMQG
>Mature_373_residues
SQSPSSRRPVLRALVAGAALSALSFPALSLAAKKTAKSPKRRVAVHEEEIDPDRYRNNPQTQAFIGMLVAQYNFDRTSLE
TLFAGTAYSATVARLILPPATPARRSWTAYRGRFIEPIRIGAGVRFWQQYGDTLRRAENEFGVPADVIVGILGVETIYGR
DMGNFRVMDALSTLAFDYPDTPNREDRSTMFRNQLKDFLLWCRDTGTETFSVLGSYAGAVGIPQFMPTSIREYAIDYDHD
GHIDLRNSAVDAIGSVARFLQMHGWEPNRPVMWNIAGDADSQGIAAAAADGQPYPGMTLSRLTRAGLALAPGVDTAREQE
TEVLMIDLPTPGQPTEYRVGLRNFYVLTRYNRSFFYAAAVYELGQAVRQAMQG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=330, Percent_Identity=40.3030303030303, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41422; Mature: 41291

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSPSSRRPVLRALVAGAALSALSFPALSLAAKKTAKSPKRRVAVHEEEIDPDRYRNNP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCHHCCCHHHHCCCC
QTQAFIGMLVAQYNFDRTSLETLFAGTAYSATVARLILPPATPARRSWTAYRGRFIEPIR
HHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEE
IGAGVRFWQQYGDTLRRAENEFGVPADVIVGILGVETIYGRDMGNFRVMDALSTLAFDYP
ECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DTPNREDRSTMFRNQLKDFLLWCRDTGTETFSVLGSYAGAVGIPQFMPTSIREYAIDYDH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCC
DGHIDLRNSAVDAIGSVARFLQMHGWEPNRPVMWNIAGDADSQGIAAAAADGQPYPGMTL
CCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCHH
SRLTRAGLALAPGVDTAREQETEVLMIDLPTPGQPTEYRVGLRNFYVLTRYNRSFFYAAA
HHHHHHCCEECCCCCCCCCCCCCEEEEECCCCCCCCCEEECCEEEEEEEEECCCHHHHHH
VYELGQAVRQAMQG
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SQSPSSRRPVLRALVAGAALSALSFPALSLAAKKTAKSPKRRVAVHEEEIDPDRYRNNP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCHHCCCHHHHCCCC
QTQAFIGMLVAQYNFDRTSLETLFAGTAYSATVARLILPPATPARRSWTAYRGRFIEPIR
HHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEE
IGAGVRFWQQYGDTLRRAENEFGVPADVIVGILGVETIYGRDMGNFRVMDALSTLAFDYP
ECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DTPNREDRSTMFRNQLKDFLLWCRDTGTETFSVLGSYAGAVGIPQFMPTSIREYAIDYDH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCC
DGHIDLRNSAVDAIGSVARFLQMHGWEPNRPVMWNIAGDADSQGIAAAAADGQPYPGMTL
CCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCHH
SRLTRAGLALAPGVDTAREQETEVLMIDLPTPGQPTEYRVGLRNFYVLTRYNRSFFYAAA
HHHHHHCCEECCCCCCCCCCCCCEEEEECCCCCCCCCEEECCEEEEEEEEECCCHHHHHH
VYELGQAVRQAMQG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]