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The map label for this gene is gph2 [H]

Identifier: 187927863

GI number: 187927863

Start: 811703

End: 812392

Strand: Reverse

Name: gph2 [H]

Synonym: Rpic_0767

Alternate gene names: 187927863

Gene position: 812392-811703 (Counterclockwise)

Preceding gene: 187927864

Following gene: 187927862

Centisome position: 20.61

GC content: 67.83

Gene sequence:

>690_bases
ATGACGGCGCGCCAGTTTCCCGATCCGCTGGGCGCGGTGCTGTTCGACCTGGACGGCACACTTGCGGACACGGCCCCCGA
TCTGGCCGCCGCGGCCAACAAGGTGCGCACTGATCGTGGCCTCGAACCGGTGGACTACGAAGCGCTGCGCCCGGTCGCCT
CGCATGGTGCCCGCGGACTGATCGGCGTGGCCTTCGGTGTTAGGCCAGGGGATGCGGAGTTCGAGTCGCTACGGCTGGCG
TTTCTCGCCAACTACGAGGCGGAAATCTGTGTGCGTACGCAGCTGTTTCCGGGCATGGCCGAAGTGCTGGTCGAACTGGG
GAGGGCGGGCGTCCCCTGGGGGATCGTCACCAACAAGTCGGGCCGCCTGACGGTACCGCTGGTTGCACGGTTGCCGTTGC
CGGTGCCGCCGGCGTGCGTCGTTGCGGGCGACACGACGCCGCACGCCAAGCCCCACCCCGCCCCACTGCTGCATGCGGCC
GAATCCATCGGCATCGACGCACGACAGATCGTCTACGTGGGCGATGACCTGCGCGATATCGAAGCCGGCCGCGCTGCCGG
CATGCCTACCATTGCGGCCAGTTACGGCTATTGTGGTAATGGGCCATCGCCAGCGGAATGGCGCGCCGATGCGCTGATCG
CCAGCGCGGCCGAGCTGATTCCGCTGCTGCTCGAGTCGCAACGCGCTTGA

Upstream 100 bases:

>100_bases
AGATGTGCGGGCTCGAATACAACCCGATCACCGGCCGCTATGCCCTCACGCAGGACGCCAGCGTCAACTATTTGATGGCA
ACGCGCCGTCCGGCCGAGGC

Downstream 100 bases:

>100_bases
CCACAATGCCCAGGTGAATGCCCGGGCGCCACACCTTTCAGGACCGCTGCCTCCCGTCGGGACGTGGCGGTCAATCGACT
GACGAAGTAGGCCACGCCGT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTARQFPDPLGAVLFDLDGTLADTAPDLAAAANKVRTDRGLEPVDYEALRPVASHGARGLIGVAFGVRPGDAEFESLRLA
FLANYEAEICVRTQLFPGMAEVLVELGRAGVPWGIVTNKSGRLTVPLVARLPLPVPPACVVAGDTTPHAKPHPAPLLHAA
ESIGIDARQIVYVGDDLRDIEAGRAAGMPTIAASYGYCGNGPSPAEWRADALIASAAELIPLLLESQRA

Sequences:

>Translated_229_residues
MTARQFPDPLGAVLFDLDGTLADTAPDLAAAANKVRTDRGLEPVDYEALRPVASHGARGLIGVAFGVRPGDAEFESLRLA
FLANYEAEICVRTQLFPGMAEVLVELGRAGVPWGIVTNKSGRLTVPLVARLPLPVPPACVVAGDTTPHAKPHPAPLLHAA
ESIGIDARQIVYVGDDLRDIEAGRAAGMPTIAASYGYCGNGPSPAEWRADALIASAAELIPLLLESQRA
>Mature_228_residues
TARQFPDPLGAVLFDLDGTLADTAPDLAAAANKVRTDRGLEPVDYEALRPVASHGARGLIGVAFGVRPGDAEFESLRLAF
LANYEAEICVRTQLFPGMAEVLVELGRAGVPWGIVTNKSGRLTVPLVARLPLPVPPACVVAGDTTPHAKPHPAPLLHAAE
SIGIDARQIVYVGDDLRDIEAGRAAGMPTIAASYGYCGNGPSPAEWRADALIASAAELIPLLLESQRA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=240, Percent_Identity=34.1666666666667, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23988; Mature: 23856

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARQFPDPLGAVLFDLDGTLADTAPDLAAAANKVRTDRGLEPVDYEALRPVASHGARGL
CCCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCE
IGVAFGVRPGDAEFESLRLAFLANYEAEICVRTQLFPGMAEVLVELGRAGVPWGIVTNKS
EEEEECCCCCCCHHHHEEEEEEECCCCEEEEEEHHCCCHHHHHHHHCCCCCCEEEEECCC
GRLTVPLVARLPLPVPPACVVAGDTTPHAKPHPAPLLHAAESIGIDARQIVYVGDDLRDI
CCEEEEEHEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHH
EAGRAAGMPTIAASYGYCGNGPSPAEWRADALIASAAELIPLLLESQRA
HCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TARQFPDPLGAVLFDLDGTLADTAPDLAAAANKVRTDRGLEPVDYEALRPVASHGARGL
CCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCE
IGVAFGVRPGDAEFESLRLAFLANYEAEICVRTQLFPGMAEVLVELGRAGVPWGIVTNKS
EEEEECCCCCCCHHHHEEEEEEECCCCEEEEEEHHCCCHHHHHHHHCCCCCCEEEEECCC
GRLTVPLVARLPLPVPPACVVAGDTTPHAKPHPAPLLHAAESIGIDARQIVYVGDDLRDI
CCEEEEEHEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHH
EAGRAAGMPTIAASYGYCGNGPSPAEWRADALIASAAELIPLLLESQRA
HCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]