Click here to switch to the map view.

The map label for this gene is glnS [H]

Identifier: 187927833

GI number: 187927833

Start: 772294

End: 774024

Strand: Reverse

Name: glnS [H]

Synonym: Rpic_0737

Alternate gene names: 187927833

Gene position: 774024-772294 (Counterclockwise)

Preceding gene: 187927834

Following gene: 187927832

Centisome position: 19.63

GC content: 64.7

Gene sequence:

>1731_bases
ATGAGCCAAGACAACGCCACCGCCCCGACGTCCAACTTCCTGCGCCAGATCATTGACGCCGACCTCGCGCAAGGCACCTA
CGCCGATCGCAAGGACGCCACTGGCCAGCCGATTCCGCCGGTGGTCACGCGCTTTCCGCCGGAACCCAACGGTTACCTGC
ACATCGGTCACGCCAAGAGCATCTGGGTGAACTTCGGCATGGCCCGCGATTACAACGGCCGCTGCCACCTGCGCTTCGAC
GACACGAACCCCGTCAAGGAAGACACCGAATACGTCGATTCCATCATCGACGCCGTGCACTGGCTCGGCTATTCGTGGAG
CGATGCCGGCGGCGAACACCTCTACTACGCCAGCGACTACTTCGAGCAGCTCTACGGCTTTGCCGAGGTGCTTATCCAGC
GCGGCGTGGCCTACGTCGACAGCCAGAGCGCCGAGCAGATCGCCGCCAACCGGGGCGATTTCACCCGCCCGGGCACACTG
TCGCCGTTCCGTGACCGCTCGGTGGACGAAAACCTGGCGCTGTTCCGCGACATGCGCGCCGGCAAGTACAAGGACGGCGA
GCACGTGCTGCGCGCCAAGATCGACATGGCCGCGCCGAACATCGTGATGCGCGACCCGGTGCTGTACCGCATCCGCCATG
CGCATCACCACCGCACCGGCGACGCGTGGTGCATCTACCCGATGTACGACTTCACGCATTGCATTTCCGATGCGCTGGAG
AACATCACGCATTCGCTCTGCACGCTGGAGTTCGAGAACAACCGCCCGCTGTATGACTGGGTGCTCGACCACCTGCGTGA
TGCCGGCGTGCTGGCCGCCCCGCTGCCGCATCAATACGAATTCGCGCGCCTGCACCTGACCTACGCCATCACCAGCAAGC
GCAAGCTGCTGCAACTGGTGACGGAAAAGCGCGTCGACGGCTGGGACGACCCGCGCATGCCGACGCTCGTTGGCATCCGC
CGCCGTGGCTACACGCCCGAATCGATCCAGCTGTTCTGCGAACGCGTGGGTGTGTCGAAGGCCGACAGCTGGATCGACAT
GAGCATCCTGGAAGGCGCCGTGCGTGACGACCTCGACGCGCGTGCGCCGCGCTCGGTGGCCGTGCTGGACCCGGTCAAGC
TGGTGCTCGACAACGTGCCGGCCGATTTCAACGAGCCGTGCTCCGCACCCGTGCACCCGAAGCAACCCGAACTCGGCCGC
CGCGAATTCCCGCTCACGCGTGAGCTGTGGATCGAGCGCGAGGACTTTACCGAGACGCCGCCCAAGGGCTACTTCCGCCT
CTTCCCGGGCAACAAGGTGCGCCTGCGCTACGGCTATGTGATCGAATGCACCGGCTGCGACAAGGATGCCGCCGGCAACA
TCACCGCCGTGCACGCCAACATCATCCCGGACACGAAGAGTGGCACGCCGGGTGCTGACAGCGTGAAGGTGAAGGGCAAC
ATCCACTGGGTGAGCGCCGCACATGCGCTGGAAGCTGAAGTGCGCCTGTACGACCGCCTGTTCAGCGACCCGCAGCCGGA
TTCGGGCGACAAGAACTTCCTGGATGCGCTCAACCCGAACTCCAAGAAGATCGTCAAGGCCTTCCTGGAGCCGACGCTGG
CTACGGCCAAGGCGGAAGACCGCTTCCAGTTCGAGCGCCATGGCTACTTCGTGGCAGACCGCATCGACTCGCAGCCGGGC
AAGCCCGTGTTCAACCGCGTGGTCGGCCTGAAGGACAGCTGGGGCAAGTAA

Upstream 100 bases:

>100_bases
GCGCACAGGCGCTCGATGGCCTGGCCCAGCGTAGATAGCGTCCCGGGGCGCGGAGCGCCGGGCACGGTAAAATCCCGGGA
TTGCCGAATCACGCACTCCC

Downstream 100 bases:

>100_bases
GCACAGGCCCATGCGCGCCATGGGCCTCTGATATCGGAGGCATCATGCGTACATGGATCGTATTGCTGGCGGGGTTGTTC
ACCACCCCCGCCCTCGCCGC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSQDNATAPTSNFLRQIIDADLAQGTYADRKDATGQPIPPVVTRFPPEPNGYLHIGHAKSIWVNFGMARDYNGRCHLRFD
DTNPVKEDTEYVDSIIDAVHWLGYSWSDAGGEHLYYASDYFEQLYGFAEVLIQRGVAYVDSQSAEQIAANRGDFTRPGTL
SPFRDRSVDENLALFRDMRAGKYKDGEHVLRAKIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALE
NITHSLCTLEFENNRPLYDWVLDHLRDAGVLAAPLPHQYEFARLHLTYAITSKRKLLQLVTEKRVDGWDDPRMPTLVGIR
RRGYTPESIQLFCERVGVSKADSWIDMSILEGAVRDDLDARAPRSVAVLDPVKLVLDNVPADFNEPCSAPVHPKQPELGR
REFPLTRELWIEREDFTETPPKGYFRLFPGNKVRLRYGYVIECTGCDKDAAGNITAVHANIIPDTKSGTPGADSVKVKGN
IHWVSAAHALEAEVRLYDRLFSDPQPDSGDKNFLDALNPNSKKIVKAFLEPTLATAKAEDRFQFERHGYFVADRIDSQPG
KPVFNRVVGLKDSWGK

Sequences:

>Translated_576_residues
MSQDNATAPTSNFLRQIIDADLAQGTYADRKDATGQPIPPVVTRFPPEPNGYLHIGHAKSIWVNFGMARDYNGRCHLRFD
DTNPVKEDTEYVDSIIDAVHWLGYSWSDAGGEHLYYASDYFEQLYGFAEVLIQRGVAYVDSQSAEQIAANRGDFTRPGTL
SPFRDRSVDENLALFRDMRAGKYKDGEHVLRAKIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALE
NITHSLCTLEFENNRPLYDWVLDHLRDAGVLAAPLPHQYEFARLHLTYAITSKRKLLQLVTEKRVDGWDDPRMPTLVGIR
RRGYTPESIQLFCERVGVSKADSWIDMSILEGAVRDDLDARAPRSVAVLDPVKLVLDNVPADFNEPCSAPVHPKQPELGR
REFPLTRELWIEREDFTETPPKGYFRLFPGNKVRLRYGYVIECTGCDKDAAGNITAVHANIIPDTKSGTPGADSVKVKGN
IHWVSAAHALEAEVRLYDRLFSDPQPDSGDKNFLDALNPNSKKIVKAFLEPTLATAKAEDRFQFERHGYFVADRIDSQPG
KPVFNRVVGLKDSWGK
>Mature_575_residues
SQDNATAPTSNFLRQIIDADLAQGTYADRKDATGQPIPPVVTRFPPEPNGYLHIGHAKSIWVNFGMARDYNGRCHLRFDD
TNPVKEDTEYVDSIIDAVHWLGYSWSDAGGEHLYYASDYFEQLYGFAEVLIQRGVAYVDSQSAEQIAANRGDFTRPGTLS
PFRDRSVDENLALFRDMRAGKYKDGEHVLRAKIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALEN
ITHSLCTLEFENNRPLYDWVLDHLRDAGVLAAPLPHQYEFARLHLTYAITSKRKLLQLVTEKRVDGWDDPRMPTLVGIRR
RGYTPESIQLFCERVGVSKADSWIDMSILEGAVRDDLDARAPRSVAVLDPVKLVLDNVPADFNEPCSAPVHPKQPELGRR
EFPLTRELWIEREDFTETPPKGYFRLFPGNKVRLRYGYVIECTGCDKDAAGNITAVHANIIPDTKSGTPGADSVKVKGNI
HWVSAAHALEAEVRLYDRLFSDPQPDSGDKNFLDALNPNSKKIVKAFLEPTLATAKAEDRFQFERHGYFVADRIDSQPGK
PVFNRVVGLKDSWGK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=541, Percent_Identity=45.8410351201479, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI62241042, Length=532, Percent_Identity=34.0225563909774, Blast_Score=268, Evalue=8e-72,
Organism=Escherichia coli, GI1786895, Length=572, Percent_Identity=58.041958041958, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=317, Percent_Identity=23.3438485804416, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543332, Length=541, Percent_Identity=43.2532347504621, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17511007, Length=520, Percent_Identity=31.1538461538462, Blast_Score=226, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324742, Length=574, Percent_Identity=42.6829268292683, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321192, Length=528, Percent_Identity=35.4166666666667, Blast_Score=285, Evalue=1e-77,
Organism=Drosophila melanogaster, GI17864488, Length=540, Percent_Identity=42.7777777777778, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649466, Length=526, Percent_Identity=32.8897338403042, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 65203; Mature: 65072

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDNATAPTSNFLRQIIDADLAQGTYADRKDATGQPIPPVVTRFPPEPNGYLHIGHAKS
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCE
IWVNFGMARDYNGRCHLRFDDTNPVKEDTEYVDSIIDAVHWLGYSWSDAGGEHLYYASDY
EEEEECEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHH
FEQLYGFAEVLIQRGVAYVDSQSAEQIAANRGDFTRPGTLSPFRDRSVDENLALFRDMRA
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GKYKDGEHVLRAKIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALE
CCCCCCCHHHHEEHHHCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
NITHSLCTLEFENNRPLYDWVLDHLRDAGVLAAPLPHQYEFARLHLTYAITSKRKLLQLV
HHHHHHEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEEEEHHHHHHHHHH
TEKRVDGWDDPRMPTLVGIRRRGYTPESIQLFCERVGVSKADSWIDMSILEGAVRDDLDA
HHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
RAPRSVAVLDPVKLVLDNVPADFNEPCSAPVHPKQPELGRREFPLTRELWIEREDFTETP
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCC
PKGYFRLFPGNKVRLRYGYVIECTGCDKDAAGNITAVHANIIPDTKSGTPGADSVKVKGN
CCCEEEECCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECC
IHWVSAAHALEAEVRLYDRLFSDPQPDSGDKNFLDALNPNSKKIVKAFLEPTLATAKAED
EEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
RFQFERHGYFVADRIDSQPGKPVFNRVVGLKDSWGK
HHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQDNATAPTSNFLRQIIDADLAQGTYADRKDATGQPIPPVVTRFPPEPNGYLHIGHAKS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCE
IWVNFGMARDYNGRCHLRFDDTNPVKEDTEYVDSIIDAVHWLGYSWSDAGGEHLYYASDY
EEEEECEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHH
FEQLYGFAEVLIQRGVAYVDSQSAEQIAANRGDFTRPGTLSPFRDRSVDENLALFRDMRA
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GKYKDGEHVLRAKIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALE
CCCCCCCHHHHEEHHHCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
NITHSLCTLEFENNRPLYDWVLDHLRDAGVLAAPLPHQYEFARLHLTYAITSKRKLLQLV
HHHHHHEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEEEEHHHHHHHHHH
TEKRVDGWDDPRMPTLVGIRRRGYTPESIQLFCERVGVSKADSWIDMSILEGAVRDDLDA
HHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
RAPRSVAVLDPVKLVLDNVPADFNEPCSAPVHPKQPELGRREFPLTRELWIEREDFTETP
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCC
PKGYFRLFPGNKVRLRYGYVIECTGCDKDAAGNITAVHANIIPDTKSGTPGADSVKVKGN
CCCEEEECCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECC
IHWVSAAHALEAEVRLYDRLFSDPQPDSGDKNFLDALNPNSKKIVKAFLEPTLATAKAED
EEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
RFQFERHGYFVADRIDSQPGKPVFNRVVGLKDSWGK
HHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]