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The map label for this gene is queC

Identifier: 187927783

GI number: 187927783

Start: 724964

End: 725638

Strand: Direct

Name: queC

Synonym: Rpic_0687

Alternate gene names: 187927783

Gene position: 724964-725638 (Clockwise)

Preceding gene: 187927782

Following gene: 187927784

Centisome position: 18.39

GC content: 65.04

Gene sequence:

>675_bases
ATGAAAAAACGCGCCATCGTCCTGCTTTCAGGCGGCCTGGACTCTGCCACCGTGCTTGCCATGGCCAATGCCGACGGCTT
TGAGACTTACGCGCTGTCGATGCGCTACGGCCAGCGCCATTCGTCCGAGTTGGAAGCAGCCAAGAAAGTGGCGGCGGCGC
TCGGCGCAGTGCGCCATGAAATCGTCGATCTGGACTTGCGCAAGTTCGGGGGCTCCGCGTTGACGGACGATGCGCTCGAT
GTACCGACCGACGGTGTGCAATCGGGCATCCCGATCACGTATGTGCCGGCACGCAACACGATCATGCTGTCGCTGGCGCT
TGGCTGGGCCGAGGCGGTTGGCGCACGCGACCTGTTCTTCGGCGCGAATGCCGTGGACTACTCCGGCTATCCGGATTGCC
GTCCCGAATACGTTGCCGCGTACGAAACGCTGGCCAACCTGGCGACGAAGGCCGGCGTGGAAGGCGAGCGCATCCGCGTG
AACGCACCCATCATCAACATGACCAAGGCGGAGATCATCCAGGCCGGTGTGCGGCTGGGCGTGGACTACGGCCTGACCGT
GTCGTGCTACAAGGCCGACGACGCCGGCCGCGCTTGCGGCGTGTGCGATTCGTGCCGCATCCGCAAGGCCGGCTTTGAAG
CCGCGGGCGTGCCCGACCCCACCCGCTACGTCTGA

Upstream 100 bases:

>100_bases
ATGGACCGCTGAATAGCGGGCATATGCCCGGTTGGGCGCGGGCAGGGCGGCGGCACGCTAAAATGAGGGCCTTCCCGATT
TGCTTTGCAAGGCTTCGCCC

Downstream 100 bases:

>100_bases
GGCACCATGCCCGATATCGATCTCAACGTGTTGTCCCACACAGTGCTGTGGGGCACGTTTGCGCTGACGTTCGTGTTTGG
CGCGATCCTTCAGCGCACGC

Product: exsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKKRAIVLLSGGLDSATVLAMANADGFETYALSMRYGQRHSSELEAAKKVAAALGAVRHEIVDLDLRKFGGSALTDDALD
VPTDGVQSGIPITYVPARNTIMLSLALGWAEAVGARDLFFGANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGERIRV
NAPIINMTKAEIIQAGVRLGVDYGLTVSCYKADDAGRACGVCDSCRIRKAGFEAAGVPDPTRYV

Sequences:

>Translated_224_residues
MKKRAIVLLSGGLDSATVLAMANADGFETYALSMRYGQRHSSELEAAKKVAAALGAVRHEIVDLDLRKFGGSALTDDALD
VPTDGVQSGIPITYVPARNTIMLSLALGWAEAVGARDLFFGANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGERIRV
NAPIINMTKAEIIQAGVRLGVDYGLTVSCYKADDAGRACGVCDSCRIRKAGFEAAGVPDPTRYV
>Mature_224_residues
MKKRAIVLLSGGLDSATVLAMANADGFETYALSMRYGQRHSSELEAAKKVAAALGAVRHEIVDLDLRKFGGSALTDDALD
VPTDGVQSGIPITYVPARNTIMLSLALGWAEAVGARDLFFGANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGERIRV
NAPIINMTKAEIIQAGVRLGVDYGLTVSCYKADDAGRACGVCDSCRIRKAGFEAAGVPDPTRYV

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=224, Percent_Identity=36.1607142857143, Blast_Score=126, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_RALPJ (B2U7H6)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898270.1
- GeneID:   6286561
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0687
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- ProtClustDB:   CLSK896663
- HAMAP:   MF_01633_B
- InterPro:   IPR001518
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 23713; Mature: 23713

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRAIVLLSGGLDSATVLAMANADGFETYALSMRYGQRHSSELEAAKKVAAALGAVRHE
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHH
IVDLDLRKFGGSALTDDALDVPTDGVQSGIPITYVPARNTIMLSLALGWAEAVGARDLFF
HHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEEEHHHHHHCCCCCEEE
GANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGERIRVNAPIINMTKAEIIQAGVRLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECHHHHHHHHHHHCC
VDYGLTVSCYKADDAGRACGVCDSCRIRKAGFEAAGVPDPTRYV
CCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKRAIVLLSGGLDSATVLAMANADGFETYALSMRYGQRHSSELEAAKKVAAALGAVRHE
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHH
IVDLDLRKFGGSALTDDALDVPTDGVQSGIPITYVPARNTIMLSLALGWAEAVGARDLFF
HHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEEEHHHHHHCCCCCEEE
GANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGERIRVNAPIINMTKAEIIQAGVRLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECHHHHHHHHHHHCC
VDYGLTVSCYKADDAGRACGVCDSCRIRKAGFEAAGVPDPTRYV
CCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA