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The map label for this gene is rsuA [H]

Identifier: 187927714

GI number: 187927714

Start: 659863

End: 660609

Strand: Direct

Name: rsuA [H]

Synonym: Rpic_0618

Alternate gene names: 187927714

Gene position: 659863-660609 (Clockwise)

Preceding gene: 187927712

Following gene: 187927715

Centisome position: 16.74

GC content: 65.86

Gene sequence:

>747_bases
ATGGCTGATTCTTCTCGTCTACCGCTGTATCGCATCCTGCAATCCCAGGGCTTCGGCACGCGCCGCTATTGCAAGGACCT
CGTCCTCGCGGGGTTGGTCTTCATCAACGGCGTCGAGATGGAAAGCCCCGATGCGCTGGTCGATACCGCCGGCCTGGTCC
TGGATGTCGACGGTGAGCGCTGGGCATACCACGCCCGCGCCTATCTGATGCTCAACAAGCCGGCCGGCGTGGAGTGCTCG
CAAAAGCCGAAACATCATCCGAGCGTGTACACGCTGCTGCCGGTGCCGCTGCGTGAGCGCGCCGTGCAGTGCGTCGGGCG
GCTCGACCAGGACACCACGGGGCTGCTGCTGCTGACCGACGACGGCCAGTTCGTTCACACCATCACCAGCCCGCGCCACC
AGGTGCCCAAGGTGTATGAAATCACCACCGCCGAGCCCGTGACCGAGGCGCAGATCGCCCAACTGTACGCCGGCGTGCTG
CTGGCCGACGAAAATGAAAACGTTGCCGCCGCCTGGGCCGAGCGCGTCGACACGCACCACCTGCGCATGGCCCTCACGCA
GGGCAAATACCATCAGGTGAAACGCATGGTGGCGGCCGCCGGCAACCACGTGGACGGATTGCACCGCAGCGCCATCGGCG
GGTTGTCGCTGGGTGATCTGGCGCCGGGCGAATGGCGCTGGCTGGAAGCCGCGGACTTGGAGGCGCTGAACATCACGCCG
CCGCACGCAACCGTGACGAGTGCCTAA

Upstream 100 bases:

>100_bases
GCTTGGGGCGGTGGAGGGCATCGGATGGAATCGGTTGCTCTTTAGACTGGCCGAGCGTATCGCAAAATTCGCCCCTTTTT
GTCTTGTTCACGTTGTTGTC

Downstream 100 bases:

>100_bases
AGCGCCACAATGCGCCACCCACAAAAAACCTTGCGCCGAGGGCCGATTCCTCCAAATGAGAGGGGCAGCGGCCGAGGGCT
TTTGGTAAACTCCCCCCATG

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MADSSRLPLYRILQSQGFGTRRYCKDLVLAGLVFINGVEMESPDALVDTAGLVLDVDGERWAYHARAYLMLNKPAGVECS
QKPKHHPSVYTLLPVPLRERAVQCVGRLDQDTTGLLLLTDDGQFVHTITSPRHQVPKVYEITTAEPVTEAQIAQLYAGVL
LADENENVAAAWAERVDTHHLRMALTQGKYHQVKRMVAAAGNHVDGLHRSAIGGLSLGDLAPGEWRWLEAADLEALNITP
PHATVTSA

Sequences:

>Translated_248_residues
MADSSRLPLYRILQSQGFGTRRYCKDLVLAGLVFINGVEMESPDALVDTAGLVLDVDGERWAYHARAYLMLNKPAGVECS
QKPKHHPSVYTLLPVPLRERAVQCVGRLDQDTTGLLLLTDDGQFVHTITSPRHQVPKVYEITTAEPVTEAQIAQLYAGVL
LADENENVAAAWAERVDTHHLRMALTQGKYHQVKRMVAAAGNHVDGLHRSAIGGLSLGDLAPGEWRWLEAADLEALNITP
PHATVTSA
>Mature_247_residues
ADSSRLPLYRILQSQGFGTRRYCKDLVLAGLVFINGVEMESPDALVDTAGLVLDVDGERWAYHARAYLMLNKPAGVECSQ
KPKHHPSVYTLLPVPLRERAVQCVGRLDQDTTGLLLLTDDGQFVHTITSPRHQVPKVYEITTAEPVTEAQIAQLYAGVLL
ADENENVAAAWAERVDTHHLRMALTQGKYHQVKRMVAAAGNHVDGLHRSAIGGLSLGDLAPGEWRWLEAADLEALNITPP
HATVTSA

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=174, Percent_Identity=45.4022988505747, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787524, Length=253, Percent_Identity=29.6442687747036, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=31.4917127071823, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27172; Mature: 27041

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSSRLPLYRILQSQGFGTRRYCKDLVLAGLVFINGVEMESPDALVDTAGLVLDVDGER
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEECCCEEEEECCCC
WAYHARAYLMLNKPAGVECSQKPKHHPSVYTLLPVPLRERAVQCVGRLDQDTTGLLLLTD
EEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEC
DGQFVHTITSPRHQVPKVYEITTAEPVTEAQIAQLYAGVLLADENENVAAAWAERVDTHH
CCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
LRMALTQGKYHQVKRMVAAAGNHVDGLHRSAIGGLSLGDLAPGEWRWLEAADLEALNITP
HEEEECCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCC
PHATVTSA
CCCEECCC
>Mature Secondary Structure 
ADSSRLPLYRILQSQGFGTRRYCKDLVLAGLVFINGVEMESPDALVDTAGLVLDVDGER
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEECCCEEEEECCCC
WAYHARAYLMLNKPAGVECSQKPKHHPSVYTLLPVPLRERAVQCVGRLDQDTTGLLLLTD
EEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEC
DGQFVHTITSPRHQVPKVYEITTAEPVTEAQIAQLYAGVLLADENENVAAAWAERVDTHH
CCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
LRMALTQGKYHQVKRMVAAAGNHVDGLHRSAIGGLSLGDLAPGEWRWLEAADLEALNITP
HEEEECCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCC
PHATVTSA
CCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]