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The map label for this gene is dapF

Identifier: 187927149

GI number: 187927149

Start: 51188

End: 52066

Strand: Direct

Name: dapF

Synonym: Rpic_0039

Alternate gene names: 187927149

Gene position: 51188-52066 (Clockwise)

Preceding gene: 187927148

Following gene: 187927150

Centisome position: 1.3

GC content: 66.33

Gene sequence:

>879_bases
ATGAAACTGCACTTCACCAAGATGCACGGCGCGGGCAACGACTTTGTCGTGCTCGACGGCATCCAGACCCCGATCGACTT
CACGCCCGAGCAATGGGCTGCCATTGCCGACCGCCACTTCGGCGTGGGGGCGGACCAGCTCCTGCTGGTCGAGCGTTCGA
CACGGCCCGATGTCGACTTCCGTTACCGCATCTTCAACCACGACGGCGGCGAGGTGGAGCACTGCGGTAACGGCGCACGT
TGCTTCGTCAAGTTCGTCACCGACCGCGGTTTGACCGACAAACGCACCGTGCGTGTGGAAGTCATGAACGGCATCGCCAC
GCTGACGATGCAGGACGACGGCCAGGTCACAGTCGACATGGGCGCACCGGTGTTCGAGGCGGCGCGCCTGCCGTTCCTGC
CTGAAGGCCTGCCCACCCGCACCCAAGGCGACGACACGCAGCACGCGCTGCAGATCAACGGCCGGACCGAATGGCTGTCG
ACCGTGTCGATGGGCAATCCGCACGCCGTGCAGATTGTCGACGATACGGAAGCCTTCCCCGTGCTGGAAGATGGCCCGCT
GATCGAATCGCATGCCGTGTTTCCGCGTCGCGTGAATGCGGGCTTCATGCAGATCGTCGATCGCCATTCCGTGCGCCTGC
GCGTGTACGAGCGCGGCGCGGGGGAGACGCTCGCCTGTGGCACCGGCGCGTGCGCAGCGGTGGTGGCCGGCATCCGCCGC
GGCCTGCTCGATTCACCCGTCAAGGTGGCCACGCACGGCGGCGACCTGACCATCGCGTGGGCGGGCGATGGCCAGCCGGT
CATGATGACGGGCCCCGCCACGACCGTGTTTGAAGGCACGCTGGATCTGGATGCGCTCAAGCTGACCGCTGCCCACTAG

Upstream 100 bases:

>100_bases
GCATTTATTGATGCGGGGAGCCGGCCCGCGCCAACCCCGGCCGGCTCCCTTTAGAATGGGTGTTTTCCGAACGCTCATCG
CCGCATCTGTGCGGCCAGCC

Downstream 100 bases:

>100_bases
GCGCGCGGGAACTGCCCATTCGCCTGTCTGAACAACAAGGAGACACCGTGGCATCCAACGGCAAGAACCCCATCGACGAC
TGGTATTTCGACGGCAGCAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKLHFTKMHGAGNDFVVLDGIQTPIDFTPEQWAAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEHCGNGAR
CFVKFVTDRGLTDKRTVRVEVMNGIATLTMQDDGQVTVDMGAPVFEAARLPFLPEGLPTRTQGDDTQHALQINGRTEWLS
TVSMGNPHAVQIVDDTEAFPVLEDGPLIESHAVFPRRVNAGFMQIVDRHSVRLRVYERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVATHGGDLTIAWAGDGQPVMMTGPATTVFEGTLDLDALKLTAAH

Sequences:

>Translated_292_residues
MKLHFTKMHGAGNDFVVLDGIQTPIDFTPEQWAAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEHCGNGAR
CFVKFVTDRGLTDKRTVRVEVMNGIATLTMQDDGQVTVDMGAPVFEAARLPFLPEGLPTRTQGDDTQHALQINGRTEWLS
TVSMGNPHAVQIVDDTEAFPVLEDGPLIESHAVFPRRVNAGFMQIVDRHSVRLRVYERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVATHGGDLTIAWAGDGQPVMMTGPATTVFEGTLDLDALKLTAAH
>Mature_292_residues
MKLHFTKMHGAGNDFVVLDGIQTPIDFTPEQWAAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEHCGNGAR
CFVKFVTDRGLTDKRTVRVEVMNGIATLTMQDDGQVTVDMGAPVFEAARLPFLPEGLPTRTQGDDTQHALQINGRTEWLS
TVSMGNPHAVQIVDDTEAFPVLEDGPLIESHAVFPRRVNAGFMQIVDRHSVRLRVYERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVATHGGDLTIAWAGDGQPVMMTGPATTVFEGTLDLDALKLTAAH

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=51.6014234875445, Blast_Score=288, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_RALPJ (B2UD25)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897636.1
- ProteinModelPortal:   B2UD25
- GeneID:   6289735
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0039
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31627; Mature: 31627

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHFTKMHGAGNDFVVLDGIQTPIDFTPEQWAAIADRHFGVGADQLLLVERSTRPDVDF
CEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEE
RYRIFNHDGGEVEHCGNGARCFVKFVTDRGLTDKRTVRVEVMNGIATLTMQDDGQVTVDM
EEEEEECCCCCEEECCCCCEEEEEEEECCCCCCCEEEEEEEECCEEEEEECCCCCEEEEC
GAPVFEAARLPFLPEGLPTRTQGDDTQHALQINGRTEWLSTVSMGNPHAVQIVDDTEAFP
CCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHEEEEECCCCCEEEEEEECCCCCC
VLEDGPLIESHAVFPRRVNAGFMQIVDRHSVRLRVYERGAGETLACGTGACAAVVAGIRR
EECCCCEECCCCCCCEECCCHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVATHGGDLTIAWAGDGQPVMMTGPATTVFEGTLDLDALKLTAAH
CCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MKLHFTKMHGAGNDFVVLDGIQTPIDFTPEQWAAIADRHFGVGADQLLLVERSTRPDVDF
CEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEE
RYRIFNHDGGEVEHCGNGARCFVKFVTDRGLTDKRTVRVEVMNGIATLTMQDDGQVTVDM
EEEEEECCCCCEEECCCCCEEEEEEEECCCCCCCEEEEEEEECCEEEEEECCCCCEEEEC
GAPVFEAARLPFLPEGLPTRTQGDDTQHALQINGRTEWLSTVSMGNPHAVQIVDDTEAFP
CCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHEEEEECCCCCEEEEEEECCCCCC
VLEDGPLIESHAVFPRRVNAGFMQIVDRHSVRLRVYERGAGETLACGTGACAAVVAGIRR
EECCCCEECCCCCCCEECCCHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVATHGGDLTIAWAGDGQPVMMTGPATTVFEGTLDLDALKLTAAH
CCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA