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The map label for this gene is alkK [H]

Identifier: 187927134

GI number: 187927134

Start: 34952

End: 36586

Strand: Direct

Name: alkK [H]

Synonym: Rpic_0024

Alternate gene names: 187927134

Gene position: 34952-36586 (Clockwise)

Preceding gene: 187927131

Following gene: 187927136

Centisome position: 0.89

GC content: 66.3

Gene sequence:

>1635_bases
ATGCCCACGGATTTTGATAGCGGTCTGGCCCGCAACGCGGCCAACTTCGTCCCGCTCACACCCATCGAGTTTCTTGCCCG
CGCGGCGGATGTGTATGGCGACCGCCTGGCCATCGTGCACGGGCCGGTGCGCCAGAACTGGCGCGACACCTATGAGCGCG
CGCGGCGCCTGGCCAGTGCGCTGTCGCTGGCGGGCGTCGGCCGTGGCGACACGGTGGCGGCGCTCCTGCCCAACACGCCT
GCCATGGTGGAGGCGCACTTCGGGGTGCCGATGGCGGGCGGCGTGCTCAACGCGCTGAACATCCGTCTGGATGCCGCCAA
CCTCGTCTTCATGCTGCGCCATGGCGAAGCCCGCGTGCTGCTGGCCGACACCGAGTTTGCCGACCTGGCCCGCCAGATGG
CGCTGGAGATTCCCGGCCTGAAAGTGATTGCCGTGAACGATGCCCTGGGTCCGCAGGCCGAGCCGTTTGGCGACACCGAT
TACGAAACGTTCCTGGCCTCGGGCGACCCGCACTACGCCTGGCAGTTGCCCGCCGACGAGTGGGACGCCATTGCGCTGAA
CTACACCTCGGGCACCACGGGCGACCCGAAGGGCGTGGTCTATCACCATCGAGGCGCGGCCATCAACGCGGTGTCGAACA
TCCTGGAGTGGGATCTGCCCAAGCATCCCGTCTATCTCTGGACGCTGCCGATGTTCCACTGCAATGGCTGGTGCTTCCCA
TGGACGATCGCGGCGCGTGCGGGCGTGAATGTCTGCCTGCGCAAGTTCGAGCCGAAGCTGGTGTTTGACCTCATGCGCGA
CGAAGGCGTGACGCACTACTGCGCCGCGCCCATCGTGCATACCGCGCTGGTGAACGCGCCGCCGTCGTGGCGCGAGGGCC
TGCGCGGGCCGGTGCGCGGCATGGTGGCGGGTGCACCGCCCCCGGCCGCCGTGCTGGCGCAGATGGAAGCCATGGGCTTT
GAGCTGACGCACGTCTACGGCCTGACCGAGGTGTACGGCCCTGCAGCCGTCTGCGCCGAGCAGGACGAATGGCGTACGCT
GCCCGAGCACGACCGGGCAACGATGAAGGCGCGCCAGGGCGTGCGGTACCACCTGCAATCGCAAGTGGCGGTGCTCAACC
CCGAGACCATGCAGCCTGTGCCGGCCGATGGCGAGACCATTGGCGAAATCATGTTCCGCGGCAACATCTGCATGAAGGGC
TATCTCAAGAACGAGAAGGCCACGCGCGAGGCCTTTGCCGGCGGCTGGTTCCACACCGGCGACCTGGGCGTCTGCATGCC
CGACGGCTACATCAAGATCAAGGACCGCAGCAAGGACATCATCATCTCCGGCGGCGAGAACATCTCAAGCGTGGAAGTGG
AGGACGCGCTGTATCGGCACCCGGCCGTGCTGGCTGCCGCCGTGGTTGCCCAGCCGGATGCCAAGTGGGGCGAAACGCCC
TGCGCCTTCGTTGAACTCAAGGACGGCGCCACCGCCACCGCAGAAGACCTGATCGCACATTGCAAGACGTTGCTGGCCGG
CTTCAAGGTGCCCAAGGCAGTGTTCTTCGGGCCGCTGCCCAAGACGTCGACGGGCAAGATCCAGAAATACGAGTTGCGCC
GCAAGGTGAAGTCGACTTCGGCGATTGATGTGTGA

Upstream 100 bases:

>100_bases
TTCCGGGCGGTTTGTCACTCAGGTGACAGAGTGCGGGAGCCGGCCGATGATAGCGTTGCCCAACAAAAAGCAACAGCGCC
CGGCGCACGGAGACACAGCA

Downstream 100 bases:

>100_bases
GGCTGGCCGGGGCTGCCGCGTGGGACCCCCGCAATAAAACAGAGCCCCTCGCCTTCGCGAGGGGCTTTTTGCATTCCACC
AAATCGATTCAGCCCAGCCT

Product: acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MPTDFDSGLARNAANFVPLTPIEFLARAADVYGDRLAIVHGPVRQNWRDTYERARRLASALSLAGVGRGDTVAALLPNTP
AMVEAHFGVPMAGGVLNALNIRLDAANLVFMLRHGEARVLLADTEFADLARQMALEIPGLKVIAVNDALGPQAEPFGDTD
YETFLASGDPHYAWQLPADEWDAIALNYTSGTTGDPKGVVYHHRGAAINAVSNILEWDLPKHPVYLWTLPMFHCNGWCFP
WTIAARAGVNVCLRKFEPKLVFDLMRDEGVTHYCAAPIVHTALVNAPPSWREGLRGPVRGMVAGAPPPAAVLAQMEAMGF
ELTHVYGLTEVYGPAAVCAEQDEWRTLPEHDRATMKARQGVRYHLQSQVAVLNPETMQPVPADGETIGEIMFRGNICMKG
YLKNEKATREAFAGGWFHTGDLGVCMPDGYIKIKDRSKDIIISGGENISSVEVEDALYRHPAVLAAAVVAQPDAKWGETP
CAFVELKDGATATAEDLIAHCKTLLAGFKVPKAVFFGPLPKTSTGKIQKYELRRKVKSTSAIDV

Sequences:

>Translated_544_residues
MPTDFDSGLARNAANFVPLTPIEFLARAADVYGDRLAIVHGPVRQNWRDTYERARRLASALSLAGVGRGDTVAALLPNTP
AMVEAHFGVPMAGGVLNALNIRLDAANLVFMLRHGEARVLLADTEFADLARQMALEIPGLKVIAVNDALGPQAEPFGDTD
YETFLASGDPHYAWQLPADEWDAIALNYTSGTTGDPKGVVYHHRGAAINAVSNILEWDLPKHPVYLWTLPMFHCNGWCFP
WTIAARAGVNVCLRKFEPKLVFDLMRDEGVTHYCAAPIVHTALVNAPPSWREGLRGPVRGMVAGAPPPAAVLAQMEAMGF
ELTHVYGLTEVYGPAAVCAEQDEWRTLPEHDRATMKARQGVRYHLQSQVAVLNPETMQPVPADGETIGEIMFRGNICMKG
YLKNEKATREAFAGGWFHTGDLGVCMPDGYIKIKDRSKDIIISGGENISSVEVEDALYRHPAVLAAAVVAQPDAKWGETP
CAFVELKDGATATAEDLIAHCKTLLAGFKVPKAVFFGPLPKTSTGKIQKYELRRKVKSTSAIDV
>Mature_543_residues
PTDFDSGLARNAANFVPLTPIEFLARAADVYGDRLAIVHGPVRQNWRDTYERARRLASALSLAGVGRGDTVAALLPNTPA
MVEAHFGVPMAGGVLNALNIRLDAANLVFMLRHGEARVLLADTEFADLARQMALEIPGLKVIAVNDALGPQAEPFGDTDY
ETFLASGDPHYAWQLPADEWDAIALNYTSGTTGDPKGVVYHHRGAAINAVSNILEWDLPKHPVYLWTLPMFHCNGWCFPW
TIAARAGVNVCLRKFEPKLVFDLMRDEGVTHYCAAPIVHTALVNAPPSWREGLRGPVRGMVAGAPPPAAVLAQMEAMGFE
LTHVYGLTEVYGPAAVCAEQDEWRTLPEHDRATMKARQGVRYHLQSQVAVLNPETMQPVPADGETIGEIMFRGNICMKGY
LKNEKATREAFAGGWFHTGDLGVCMPDGYIKIKDRSKDIIISGGENISSVEVEDALYRHPAVLAAAVVAQPDAKWGETPC
AFVELKDGATATAEDLIAHCKTLLAGFKVPKAVFFGPLPKTSTGKIQKYELRRKVKSTSAIDV

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=535, Percent_Identity=28.9719626168224, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI187761345, Length=547, Percent_Identity=27.2394881170018, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI187761343, Length=547, Percent_Identity=27.2394881170018, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI157311624, Length=509, Percent_Identity=26.9155206286837, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI157311622, Length=509, Percent_Identity=26.9155206286837, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI122937307, Length=512, Percent_Identity=28.3203125, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI58082049, Length=506, Percent_Identity=26.6798418972332, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI42544132, Length=502, Percent_Identity=27.0916334661355, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=25.1439539347409, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI115511026, Length=516, Percent_Identity=25.3875968992248, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI42794760, Length=586, Percent_Identity=24.2320819112628, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI42794758, Length=586, Percent_Identity=24.2320819112628, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI42794756, Length=586, Percent_Identity=24.2320819112628, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI28416953, Length=128, Percent_Identity=33.59375, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI40807491, Length=222, Percent_Identity=31.0810810810811, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI221142682, Length=521, Percent_Identity=27.2552783109405, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI145693145, Length=540, Percent_Identity=29.4444444444444, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788107, Length=555, Percent_Identity=26.8468468468468, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1786810, Length=548, Percent_Identity=27.007299270073, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=28.0373831775701, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=28.0373831775701, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=28.0373831775701, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=27.4436090225564, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=526, Percent_Identity=26.2357414448669, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=24.8565965583174, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=365, Percent_Identity=30.1369863013699, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=25.4863813229572, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=25.3875968992248, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=500, Percent_Identity=23.6, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=521, Percent_Identity=24.1842610364683, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=520, Percent_Identity=22.8846153846154, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17510401, Length=182, Percent_Identity=32.967032967033, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=560, Percent_Identity=24.6428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71985884, Length=383, Percent_Identity=29.242819843342, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=560, Percent_Identity=24.6428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=28.9693593314763, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581924, Length=557, Percent_Identity=28.0071813285458, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859661, Length=520, Percent_Identity=27.8846153846154, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=25.3308128544423, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45552801, Length=457, Percent_Identity=26.6958424507659, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550496, Length=457, Percent_Identity=26.6958424507659, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=25.3846153846154, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648260, Length=500, Percent_Identity=24.2, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583465, Length=471, Percent_Identity=23.5668789808917, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583463, Length=471, Percent_Identity=23.5668789808917, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364798, Length=470, Percent_Identity=23.1914893617021, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=495, Percent_Identity=21.6161616161616, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356947, Length=513, Percent_Identity=21.8323586744639, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=477, Percent_Identity=22.6415094339623, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=23.9782016348774, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648257, Length=474, Percent_Identity=23.8396624472574, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59187; Mature: 59056

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDFDSGLARNAANFVPLTPIEFLARAADVYGDRLAIVHGPVRQNWRDTYERARRLASA
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LSLAGVGRGDTVAALLPNTPAMVEAHFGVPMAGGVLNALNIRLDAANLVFMLRHGEARVL
HHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEEECCEEEEEEECCCEEEE
LADTEFADLARQMALEIPGLKVIAVNDALGPQAEPFGDTDYETFLASGDPHYAWQLPADE
EECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCCCEEEECCCCC
WDAIALNYTSGTTGDPKGVVYHHRGAAINAVSNILEWDLPKHPVYLWTLPMFHCNGWCFP
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCEEH
WTIAARAGVNVCLRKFEPKLVFDLMRDEGVTHYCAAPIVHTALVNAPPSWREGLRGPVRG
HHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHH
MVAGAPPPAAVLAQMEAMGFELTHVYGLTEVYGPAAVCAEQDEWRTLPEHDRATMKARQG
EECCCCCHHHHHHHHHHCCEEEEHHHHHHHHCCCHHHHCCCHHCCCCCCHHHHHHHHHCC
VRYHLQSQVAVLNPETMQPVPADGETIGEIMFRGNICMKGYLKNEKATREAFAGGWFHTG
CCEEECCCEEEECCCCCCCCCCCCHHHHHHHEECCEEEEEEECCCHHHHHHHCCCCEEEC
DLGVCMPDGYIKIKDRSKDIIISGGENISSVEVEDALYRHPAVLAAAVVAQPDAKWGETP
CEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCCCCCCC
CAFVELKDGATATAEDLIAHCKTLLAGFKVPKAVFFGPLPKTSTGKIQKYELRRKVKSTS
EEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCC
AIDV
CCCC
>Mature Secondary Structure 
PTDFDSGLARNAANFVPLTPIEFLARAADVYGDRLAIVHGPVRQNWRDTYERARRLASA
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LSLAGVGRGDTVAALLPNTPAMVEAHFGVPMAGGVLNALNIRLDAANLVFMLRHGEARVL
HHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEEECCEEEEEEECCCEEEE
LADTEFADLARQMALEIPGLKVIAVNDALGPQAEPFGDTDYETFLASGDPHYAWQLPADE
EECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCCCEEEECCCCC
WDAIALNYTSGTTGDPKGVVYHHRGAAINAVSNILEWDLPKHPVYLWTLPMFHCNGWCFP
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCEEH
WTIAARAGVNVCLRKFEPKLVFDLMRDEGVTHYCAAPIVHTALVNAPPSWREGLRGPVRG
HHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHH
MVAGAPPPAAVLAQMEAMGFELTHVYGLTEVYGPAAVCAEQDEWRTLPEHDRATMKARQG
EECCCCCHHHHHHHHHHCCEEEEHHHHHHHHCCCHHHHCCCHHCCCCCCHHHHHHHHHCC
VRYHLQSQVAVLNPETMQPVPADGETIGEIMFRGNICMKGYLKNEKATREAFAGGWFHTG
CCEEECCCEEEECCCCCCCCCCCCHHHHHHHEECCEEEEEEECCCHHHHHHHCCCCEEEC
DLGVCMPDGYIKIKDRSKDIIISGGENISSVEVEDALYRHPAVLAAAVVAQPDAKWGETP
CEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCCCCCCC
CAFVELKDGATATAEDLIAHCKTLLAGFKVPKAVFFGPLPKTSTGKIQKYELRRKVKSTS
EEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCC
AIDV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]