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The map label for this gene is prfB [H]

Identifier: 187917953

GI number: 187917953

Start: 69776

End: 70837

Strand: Direct

Name: prfB [H]

Synonym: BH0074

Alternate gene names: 187917953

Gene position: 69776-70837 (Clockwise)

Preceding gene: 187917952

Following gene: 187917954

Centisome position: 7.57

GC content: 30.7

Gene sequence:

>1062_bases
ATGAACTTTTGGAAGAAATTCAAAATGTCTGGGGGAAGCTTTGACAAAGATAAGCTCAAGACACAACTTAAAGAATATGA
GAAACAAATAAATAACGAAAATTTTTGGAATGATAATAAAAAAGCGCAAGAAATTCTTAAAAATCAAAATATTTTAAAAG
CTAAAATTGAACCTTGGGAGGACTTAATATTTAAACTCCAAGACTTAAGAGAACTCTGCGAAATCGCAGAGAGCGAAGAA
GACACAATACTACTTGATAAAGATATTAATAAACTAAAAGAACAGTATAAACATCTCTTAACAATATCTTATTTTAAAGA
AGATATTGATATAAGTAATGCGTTTTTGACAATACACTCTGGAGCAGGTGGAACAGAAGCCTGTGATTGGGTTAGCATGT
TATATAGAATATACCTAAGATACTCTGAGAGACGTGGATATAAAACTGAACTTATAGACTTACTTGAAGCTGAGGGGGGC
ATTAAATCTGTTACAATTGAAATAAAAGGAAAATATGCTTATGGATTTTTAAAAAGTGAAATAGGAATACATCGTCTTGT
AAGAATTTCTCCTTTTGATGCTGCAAAAAAGAGACACACATCTTTTGCATCTGTTTTCATTGATCCTGTAATTGATGATA
AAATTGAAATAATAATTAAACCAGAAGATATTAGGGTAGACACATATAGAGCCTCAGGAGCAGGGGGGCAACATGTCAAT
AAAACATCATCAGCTGTTAGAATTACCCATCTTCAAACGGGCATAGTAACTCAATCTCAAACTGATAGAAGTCAGCATAG
AAATAAAGAGCTAGCAATGAAAGTATTAAAATCAAAACTTTATGAACACTACAAAGCACTAGAACAACAAAAAAGTAAAT
CTAAACAAGAGGAGAAAAAAGATATATCTTGGGGAAATCAAATAAGATCTTATATCTTTCAACCTTATACCCTAGTAAAA
GATCACAGAACAAAATTTGAAAATCCAAATATTATCTCTGTTATGGATGGAAACATTGATAACTTTATAGAAGAATATCT
CAAATGGAAAAATCTAAGTTAA

Upstream 100 bases:

>100_bases
TGAAACAACTTATTTCTTTATAATGTTCATTTTATCTTTAATTCCAAATATAATTATACTAATAAATAATAGTGAGAGTT
TATAATGAAAGAAAAAATAA

Downstream 100 bases:

>100_bases
TCATAATAATATTATTTTCAATCTTAACAATAAAATTAGCATATCCTCAAAGTAATATTGAGTATGGTTTTTCTTATATC
ATAGACACACAAGATGAAAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNFWKKFKMSGGSFDKDKLKTQLKEYEKQINNENFWNDNKKAQEILKNQNILKAKIEPWEDLIFKLQDLRELCEIAESEE
DTILLDKDINKLKEQYKHLLTISYFKEDIDISNAFLTIHSGAGGTEACDWVSMLYRIYLRYSERRGYKTELIDLLEAEGG
IKSVTIEIKGKYAYGFLKSEIGIHRLVRISPFDAAKKRHTSFASVFIDPVIDDKIEIIIKPEDIRVDTYRASGAGGQHVN
KTSSAVRITHLQTGIVTQSQTDRSQHRNKELAMKVLKSKLYEHYKALEQQKSKSKQEEKKDISWGNQIRSYIFQPYTLVK
DHRTKFENPNIISVMDGNIDNFIEEYLKWKNLS

Sequences:

>Translated_353_residues
MNFWKKFKMSGGSFDKDKLKTQLKEYEKQINNENFWNDNKKAQEILKNQNILKAKIEPWEDLIFKLQDLRELCEIAESEE
DTILLDKDINKLKEQYKHLLTISYFKEDIDISNAFLTIHSGAGGTEACDWVSMLYRIYLRYSERRGYKTELIDLLEAEGG
IKSVTIEIKGKYAYGFLKSEIGIHRLVRISPFDAAKKRHTSFASVFIDPVIDDKIEIIIKPEDIRVDTYRASGAGGQHVN
KTSSAVRITHLQTGIVTQSQTDRSQHRNKELAMKVLKSKLYEHYKALEQQKSKSKQEEKKDISWGNQIRSYIFQPYTLVK
DHRTKFENPNIISVMDGNIDNFIEEYLKWKNLS
>Mature_353_residues
MNFWKKFKMSGGSFDKDKLKTQLKEYEKQINNENFWNDNKKAQEILKNQNILKAKIEPWEDLIFKLQDLRELCEIAESEE
DTILLDKDINKLKEQYKHLLTISYFKEDIDISNAFLTIHSGAGGTEACDWVSMLYRIYLRYSERRGYKTELIDLLEAEGG
IKSVTIEIKGKYAYGFLKSEIGIHRLVRISPFDAAKKRHTSFASVFIDPVIDDKIEIIIKPEDIRVDTYRASGAGGQHVN
KTSSAVRITHLQTGIVTQSQTDRSQHRNKELAMKVLKSKLYEHYKALEQQKSKSKQEEKKDISWGNQIRSYIFQPYTLVK
DHRTKFENPNIISVMDGNIDNFIEEYLKWKNLS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=295, Percent_Identity=36.6101694915254, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=339, Percent_Identity=32.4483775811209, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI166795305, Length=188, Percent_Identity=38.2978723404255, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=46.9565217391304, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=39.6761133603239, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=258, Percent_Identity=30.2325581395349, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=247, Percent_Identity=35.2226720647773, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19921226, Length=287, Percent_Identity=33.4494773519164, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41249; Mature: 41249

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFWKKFKMSGGSFDKDKLKTQLKEYEKQINNENFWNDNKKAQEILKNQNILKAKIEPWE
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCHH
DLIFKLQDLRELCEIAESEEDTILLDKDINKLKEQYKHLLTISYFKEDIDISNAFLTIHS
HHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GAGGTEACDWVSMLYRIYLRYSERRGYKTELIDLLEAEGGIKSVTIEIKGKYAYGFLKSE
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEHHHHHH
IGIHRLVRISPFDAAKKRHTSFASVFIDPVIDDKIEIIIKPEDIRVDTYRASGAGGQHVN
HCHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
KTSSAVRITHLQTGIVTQSQTDRSQHRNKELAMKVLKSKLYEHYKALEQQKSKSKQEEKK
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DISWGNQIRSYIFQPYTLVKDHRTKFENPNIISVMDGNIDNFIEEYLKWKNLS
HCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFWKKFKMSGGSFDKDKLKTQLKEYEKQINNENFWNDNKKAQEILKNQNILKAKIEPWE
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCHH
DLIFKLQDLRELCEIAESEEDTILLDKDINKLKEQYKHLLTISYFKEDIDISNAFLTIHS
HHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GAGGTEACDWVSMLYRIYLRYSERRGYKTELIDLLEAEGGIKSVTIEIKGKYAYGFLKSE
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEHHHHHH
IGIHRLVRISPFDAAKKRHTSFASVFIDPVIDDKIEIIIKPEDIRVDTYRASGAGGQHVN
HCHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
KTSSAVRITHLQTGIVTQSQTDRSQHRNKELAMKVLKSKLYEHYKALEQQKSKSKQEEKK
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DISWGNQIRSYIFQPYTLVKDHRTKFENPNIISVMDGNIDNFIEEYLKWKNLS
HCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]