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The map label for this gene is rnhB

Identifier: 187917927

GI number: 187917927

Start: 45193

End: 45738

Strand: Direct

Name: rnhB

Synonym: BH0046

Alternate gene names: 187917927

Gene position: 45193-45738 (Clockwise)

Preceding gene: 187917922

Following gene: 187917929

Centisome position: 4.9

GC content: 28.57

Gene sequence:

>546_bases
ATGATTTGTGGAATTGATGAAGTTGGACGAGGTTGCATCTTTGGACCTGTTCTTAGTGCAGCTGTTATTTTTAAAGGAAA
TCCTAATTTTTTAAATGAATTAGATGATTCAAAAAAACTTAAAAAAGCAAAAAGAGAATATCTCTCATCATTAATACTTG
AAAACTCATATTATGCGTTTGCAGAAGTATCTAATGAAACCATTGATAAAATCAATATTCATCATGCTTCGCTTCTTGCA
ATGCAAATTGCATATGAAAAGTTAGACATAAACTGTGATTTGGTACTTGTGGACGGAAAATTTATTCCAAAAATAAAAGC
TAAAAAAATTCAAGCAATAATTAAAGGAGATTCTATTATTAATGAAATAAAAGCAGCATCAATCATTGCAAAAGTACAAA
GAGACAAATTGATGGATGAATACAATAAAATTTACCCCCTATATGCACTTAACAAAAACAAAGGATATCCAACAAAGGAA
CATAAAGAGGCAATAAAAAAACATGGAATTTCAAATCTTCACAGAAGAAGTTTTAAGTTCATTTAA

Upstream 100 bases:

>100_bases
AGCCTAAAAGGCCTATTTTTTCTAAAAACAAAGCTACTCCCAATTCAATTACAAATAAGATTAATTTACTAAGAATAATT
TTATATTATAATCAATAGTT

Downstream 100 bases:

>100_bases
ATAAAATTTTAATTATCTCCATTTCTAGGTCTTCCTGATAAACGATAATTCCTATCAAAACTAAGATTATTATTTTTAAT
TCTTTCACTATGGCGGTCAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MICGIDEVGRGCIFGPVLSAAVIFKGNPNFLNELDDSKKLKKAKREYLSSLILENSYYAFAEVSNETIDKINIHHASLLA
MQIAYEKLDINCDLVLVDGKFIPKIKAKKIQAIIKGDSIINEIKAASIIAKVQRDKLMDEYNKIYPLYALNKNKGYPTKE
HKEAIKKHGISNLHRRSFKFI

Sequences:

>Translated_181_residues
MICGIDEVGRGCIFGPVLSAAVIFKGNPNFLNELDDSKKLKKAKREYLSSLILENSYYAFAEVSNETIDKINIHHASLLA
MQIAYEKLDINCDLVLVDGKFIPKIKAKKIQAIIKGDSIINEIKAASIIAKVQRDKLMDEYNKIYPLYALNKNKGYPTKE
HKEAIKKHGISNLHRRSFKFI
>Mature_181_residues
MICGIDEVGRGCIFGPVLSAAVIFKGNPNFLNELDDSKKLKKAKREYLSSLILENSYYAFAEVSNETIDKINIHHASLLA
MQIAYEKLDINCDLVLVDGKFIPKIKAKKIQAIIKGDSIINEIKAASIIAKVQRDKLMDEYNKIYPLYALNKNKGYPTKE
HKEAIKKHGISNLHRRSFKFI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=42.2222222222222, Blast_Score=137, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_BORHD (B2S1N3)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883490.1
- EnsemblBacteria:   EBBORT00000006761
- GeneID:   6276632
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0046
- GeneTree:   EBGT00050000007376
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   BHER314723:BH0046-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 20498; Mature: 20498

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICGIDEVGRGCIFGPVLSAAVIFKGNPNFLNELDDSKKLKKAKREYLSSLILENSYYAF
CCCCCHHHCCCCCHHHHHHEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
AEVSNETIDKINIHHASLLAMQIAYEKLDINCDLVLVDGKFIPKIKAKKIQAIIKGDSII
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHH
NEIKAASIIAKVQRDKLMDEYNKIYPLYALNKNKGYPTKEHKEAIKKHGISNLHRRSFKF
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCC
I
C
>Mature Secondary Structure
MICGIDEVGRGCIFGPVLSAAVIFKGNPNFLNELDDSKKLKKAKREYLSSLILENSYYAF
CCCCCHHHCCCCCHHHHHHEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
AEVSNETIDKINIHHASLLAMQIAYEKLDINCDLVLVDGKFIPKIKAKKIQAIIKGDSII
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHH
NEIKAASIIAKVQRDKLMDEYNKIYPLYALNKNKGYPTKEHKEAIKKHGISNLHRRSFKF
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA