Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 187736637

GI number: 187736637

Start: 2631809

End: 2632939

Strand: Direct

Name: menE [H]

Synonym: Amuc_2161

Alternate gene names: 187736637

Gene position: 2631809-2632939 (Clockwise)

Preceding gene: 187736636

Following gene: 187736639

Centisome position: 98.79

GC content: 64.99

Gene sequence:

>1131_bases
ATGCCCGCCACCGGAACACCCCTGCCTTGGGACCGCGGCAACGGCCCCTGCGTGATGGCCCCGCCCCATTTGAGGGGCTG
GGCGGAAGAGGCCGCCGCCTTTCTTTCCCTCCGGGGCATGGACGGAATCACAGCCTTCGCCACCTCCGGCTCTTCCGGTT
CTCCGCCCAAAGCCATTTTGTTTACCCGCCGCGCCCTGGACATATGCGCGCGTGGGGCATTGGAACATTTACATGCGGAA
AGCGGGGACTGGTGCTGCCCGCTGCCTGTATGGCATGTGGGGGGCGCCATGATTTACCTGCGCGCGGCCCTGGCCGGAAC
CGCCGTCCATACGCTCCGCGGAAAGTGGTGCCCGCAGGCTTATGCCGACCTGATGAAAAGTTCCGGAGCCAGATGGTCCT
CCCTGGTCCCCACGCAGGTAGTGGATCTGGTCAGCCTCGGCCTCCGCGCGCCTTCCACCGCCGGGTGCATTATCGTAGGC
GGCGGCGCGCTGGACACGGAGACGGGACGGAAAGCACGCACCCTTGGCTGGCCCGTAGTGCAGAGCTACGGCATGACGGA
AGCCGGCTCCCAATTAGCCACGGCCCTTCCCGGAGATTCCTTCCATACGGACCGCCTTTCCCTTCTGCCCCATTGGGAAG
CTCAGACGGACAAAGGCGGCCTCCTGCGCTTCCAGGGGAAAGGCAAATTGTTCGGCCGCCTGTTGACGCAGGCTCATGGA
GGGTTCCTTCTGGAAAGAGTCGCTCCGCAGGAGTGGTGGAGCACCCGCGACCTGGTGCGCCTGGAAGGGCGTTTGCTGAC
CTTTCTGCGCCGGGCGGACAGACTTGTCAAAGTCCTGGGGGAACTGGTGGATCCGGATGCCGTTCAGGACGTTTTGCGCC
GCAGCGTCCCGGAGGCTGTCGTGGAGGCAGTTCCACATCCGCGCGCCGGAATGGAACTGGTCGCCTGCGGCCCTTCCGCC
GCCCCCCTGAAGCAGGCCTGCCGCGAGTGGAATGCGTCGGCCCCAGGCCCCCAGCGCATCCGCGCCGTCATGGAAACGCC
CATCCCCCTCACGGTCATGGGAAAGCTGGACAGAAACCGGCTGCGCTCCCAGTTGAGCGATCATCCGGAAAAGTTGAAGG
GAATTTACTAA

Upstream 100 bases:

>100_bases
GGAAGCAGCCTATGCATGGATTGAGCATCATTTGAAGCCCCGCATGGAGGAACGCTGGCACACCCTGCGCAGAAAAGCCC
TCCGGTAAACTGCCGCCGCC

Downstream 100 bases:

>100_bases
AAGGTAATCTTCCGGGGATATTCATCAAAAGAATCCGGACCTGATGGAATGAAGAAATACGGCGGGGTTGGAATCCCCAA
TCCCTTGCCCACTTCATTCA

Product: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II-like protein

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MPATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGITAFATSGSSGSPPKAILFTRRALDICARGALEHLHAE
SGDWCCPLPVWHVGGAMIYLRAALAGTAVHTLRGKWCPQAYADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVG
GGALDTETGRKARTLGWPVVQSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDKGGLLRFQGKGKLFGRLLTQAHG
GFLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLVKVLGELVDPDAVQDVLRRSVPEAVVEAVPHPRAGMELVACGPSA
APLKQACREWNASAPGPQRIRAVMETPIPLTVMGKLDRNRLRSQLSDHPEKLKGIY

Sequences:

>Translated_376_residues
MPATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGITAFATSGSSGSPPKAILFTRRALDICARGALEHLHAE
SGDWCCPLPVWHVGGAMIYLRAALAGTAVHTLRGKWCPQAYADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVG
GGALDTETGRKARTLGWPVVQSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDKGGLLRFQGKGKLFGRLLTQAHG
GFLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLVKVLGELVDPDAVQDVLRRSVPEAVVEAVPHPRAGMELVACGPSA
APLKQACREWNASAPGPQRIRAVMETPIPLTVMGKLDRNRLRSQLSDHPEKLKGIY
>Mature_375_residues
PATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGITAFATSGSSGSPPKAILFTRRALDICARGALEHLHAES
GDWCCPLPVWHVGGAMIYLRAALAGTAVHTLRGKWCPQAYADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVGG
GALDTETGRKARTLGWPVVQSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDKGGLLRFQGKGKLFGRLLTQAHGG
FLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLVKVLGELVDPDAVQDVLRRSVPEAVVEAVPHPRAGMELVACGPSAA
PLKQACREWNASAPGPQRIRAVMETPIPLTVMGKLDRNRLRSQLSDHPEKLKGIY

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=268, Percent_Identity=30.9701492537313, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 40540; Mature: 40409

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGITAFATSGSSGSPPKAIL
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHH
FTRRALDICARGALEHLHAESGDWCCPLPVWHVGGAMIYLRAALAGTAVHTLRGKWCPQA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVGGGALDTETGRKARTLGWPVV
HHHHHHCCCCCCHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHH
QSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDKGGLLRFQGKGKLFGRLLTQAHG
HHCCCCHHCCHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCC
GFLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLVKVLGELVDPDAVQDVLRRSVPEAV
CHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VEAVPHPRAGMELVACGPSAAPLKQACREWNASAPGPQRIRAVMETPIPLTVMGKLDRNR
HHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCHHHH
LRSQLSDHPEKLKGIY
HHHHHHCCHHHHCCCC
>Mature Secondary Structure 
PATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGITAFATSGSSGSPPKAIL
CCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHH
FTRRALDICARGALEHLHAESGDWCCPLPVWHVGGAMIYLRAALAGTAVHTLRGKWCPQA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVGGGALDTETGRKARTLGWPVV
HHHHHHCCCCCCHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHH
QSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDKGGLLRFQGKGKLFGRLLTQAHG
HHCCCCHHCCHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCC
GFLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLVKVLGELVDPDAVQDVLRRSVPEAV
CHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VEAVPHPRAGMELVACGPSAAPLKQACREWNASAPGPQRIRAVMETPIPLTVMGKLDRNR
HHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCHHHH
LRSQLSDHPEKLKGIY
HHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]