Click here to switch to the map view.

The map label for this gene is tsf [H]

Identifier: 187735179

GI number: 187735179

Start: 792892

End: 793770

Strand: Reverse

Name: tsf [H]

Synonym: Amuc_0673

Alternate gene names: 187735179

Gene position: 793770-792892 (Counterclockwise)

Preceding gene: 187735180

Following gene: 187735178

Centisome position: 29.8

GC content: 59.04

Gene sequence:

>879_bases
ATGGCAGAAATTACTGCAGCTCTCGTCAAGGCTCTCCGTGACAAAACGGACGCCGGCATGATGGATTGCAAGAAAGCCCT
CAACGAATGCAACGGCGACATGGATGCCGCCGTAAAGTATCTTCGTGAGAAGGGAATTGCCAAGGCTGCGGCCAAGGCCG
ACCGTGACGCCAAGGAAGGCGTGATCCGCGCCGCTGTGGCTCCCTGCGGCTGCTCCGGCATCATCCTTGAACTCAACTGT
GAAACGGACTTCTGCGCCAAGGGGGACAAGTTCCAGGGTCTGGTGAATGACGTTGCCAACGCTCTCATGGATTCCAAGGC
CTCCACGCTGGAAGAGGCTCTCCAGGTCCCCATGGCGGAAGGCTCCACGGAAGAATATATCAAGGCCATGTGCAGCTCCA
TTGGCGAAAACATGGCTCTGCGCAAATTCGCCCGCCTGACGGCGGACGGCATCGGCGCCGTGGTCTCCTACATCCACATG
GGCGGCAAAGTGGGCGTTCTGCTGTCCATCAAGGCCGGCAAGGAAGAAACCGTCAAGTCTGATGCGTTCAAAGCCCTCGC
CAAGGATTTGACCTTGCACATCGCCGCCGCCGCTCCCAAGAGCGTCAGCTCCGATAGCCTGGATCCCGCCTTCGTGGCCG
AGGAACAGGAAATCGCCAAGCAGCAGCTCATTCAGGAAGGCAAGCCCGAACACCTGCTGGAAAAGATTCTTCCCGGCAAG
CTGAAGGCTCTGTACGCCCAGTCCTGCCTGCTGAACCAGGTATTTGTGAAGGACCCTGCCGGAAAGATGACGGTGGAGGC
CCTCCTCAACCAGGTCGGCAAGGAACTCGGCGACGATATCTCCGTCGCCTCCTTCGTCCGCTACCAGCTCGGTGCGTAA

Upstream 100 bases:

>100_bases
CCATCCGCCTGATTCTTGACTGCATTGTCAAGGCCATCAACGAAGGCAAGGGCGCATCCGCCGAAGCCTAAACCAACCAT
TTAGAAAGAACAAAAACACT

Downstream 100 bases:

>100_bases
GCTCTGAGTCAGAGTATCTCTGGAACCGTTCCCTTTGCGGGGAACGGTTCTTTTGTTTGATATGGCTCCATGGGGGGATT
TATCCATAAATTTGATTCAG

Product: translation elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREKGIAKAAAKADRDAKEGVIRAAVAPCGCSGIILELNC
ETDFCAKGDKFQGLVNDVANALMDSKASTLEEALQVPMAEGSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHM
GGKVGVLLSIKAGKEETVKSDAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEIAKQQLIQEGKPEHLLEKILPGK
LKALYAQSCLLNQVFVKDPAGKMTVEALLNQVGKELGDDISVASFVRYQLGA

Sequences:

>Translated_292_residues
MAEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREKGIAKAAAKADRDAKEGVIRAAVAPCGCSGIILELNC
ETDFCAKGDKFQGLVNDVANALMDSKASTLEEALQVPMAEGSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHM
GGKVGVLLSIKAGKEETVKSDAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEIAKQQLIQEGKPEHLLEKILPGK
LKALYAQSCLLNQVFVKDPAGKMTVEALLNQVGKELGDDISVASFVRYQLGA
>Mature_291_residues
AEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREKGIAKAAAKADRDAKEGVIRAAVAPCGCSGIILELNCE
TDFCAKGDKFQGLVNDVANALMDSKASTLEEALQVPMAEGSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHMG
GKVGVLLSIKAGKEETVKSDAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEIAKQQLIQEGKPEHLLEKILPGKL
KALYAQSCLLNQVFVKDPAGKMTVEALLNQVGKELGDDISVASFVRYQLGA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=248, Percent_Identity=29.4354838709677, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI291084500, Length=269, Percent_Identity=28.2527881040892, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI291084502, Length=100, Percent_Identity=40, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI291084498, Length=100, Percent_Identity=40, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786366, Length=291, Percent_Identity=37.4570446735395, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17561440, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921466, Length=166, Percent_Identity=34.3373493975904, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30956; Mature: 30825

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01126 EF_TS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREKGIAKAAAKADRDAKEG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
VIRAAVAPCGCSGIILELNCETDFCAKGDKFQGLVNDVANALMDSKASTLEEALQVPMAE
HHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHMGGKVGVLLSIKAGKEETVKS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
DAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEIAKQQLIQEGKPEHLLEKILPGK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
LKALYAQSCLLNQVFVKDPAGKMTVEALLNQVGKELGDDISVASFVRYQLGA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREKGIAKAAAKADRDAKEG
CHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
VIRAAVAPCGCSGIILELNCETDFCAKGDKFQGLVNDVANALMDSKASTLEEALQVPMAE
HHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHMGGKVGVLLSIKAGKEETVKS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
DAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEIAKQQLIQEGKPEHLLEKILPGK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
LKALYAQSCLLNQVFVKDPAGKMTVEALLNQVGKELGDDISVASFVRYQLGA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA