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The map label for this gene is purC

Identifier: 187735039

GI number: 187735039

Start: 627107

End: 627817

Strand: Direct

Name: purC

Synonym: Amuc_0532

Alternate gene names: 187735039

Gene position: 627107-627817 (Clockwise)

Preceding gene: 187735037

Following gene: 187735040

Centisome position: 23.54

GC content: 54.71

Gene sequence:

>711_bases
ATGGAACCTATTTACGAAGGGAAGGCCAAACGCCTTTATACGACTGACAATCCCAACGTCCTCCGCATGGAATACAAGGA
CGACGCCACTGCCGGAAACGGAGCCAAGAAGGCCCAGTTTGAAAACAAGGGCAGGATGAATAAGGCCATCACCCTGGTCA
TCTACCGCATGCTGGAAAGCAAGGGCGTGAAAACCCACCTGGTTGCGGACGTGGACGACATCAACATTGACGTAAAAAAG
GTTTCCATCCTTCCTCTGGAAGTGATCGTGCGCAACATCGCCGCCGGCTCCTTCAGCCGCCGCATGGGCGTCAAGGAAGG
CACCCCCTTCAAGAAGCCGATCGTGGAATTTTCCTACAAGGACGACAGCCTGGGCGACCCCTTCATTAATGATGACTACG
CCCGGGAAATGAACGCCGCTACGGAAGAGGAATGCGTCTTCCTGAAGAATCAGGCCCGCATCGTGAATGACGTGCTGATT
GACTTTTTCAAACAGGTCGGCCTTACGCTGGTGGACTTCAAAATCGAGTTCGGCCGCTTGGCGGAAGATCCCTCCCAAAT
CGTGCTGGCAGATGAAATTTCCCCGGATACCTGCCGCCTGTGGGACATCAAAACCGGCGAGAAGATGGACAAGGACCGCT
TCCGCCAGGACCTCGGCAACGTGATGGAAGCCTACGAAGAAGTCCTTTCCCGGCTCCAGAACAAGGCTTGA

Upstream 100 bases:

>100_bases
CTTCTTTTTCCCTCCCCTCCTTCATCCAGTTGCGGCTTGACCGTGCGGAGCGTCTGGCGTAACATCTCCGCACGCATTCA
ACCCCTGCGCAACACGTATC

Downstream 100 bases:

>100_bases
GCCTCTTCCCCCTTCCCATGGGAGCCGGTCTTCCGCCGGCCCCCATTTTTCATTTTACCACACTCTTGAGCGATATGACC
CTCCACTTTGAAGTTATCAG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKGRMNKAITLVIYRMLESKGVKTHLVADVDDINIDVKK
VSILPLEVIVRNIAAGSFSRRMGVKEGTPFKKPIVEFSYKDDSLGDPFINDDYAREMNAATEEECVFLKNQARIVNDVLI
DFFKQVGLTLVDFKIEFGRLAEDPSQIVLADEISPDTCRLWDIKTGEKMDKDRFRQDLGNVMEAYEEVLSRLQNKA

Sequences:

>Translated_236_residues
MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKGRMNKAITLVIYRMLESKGVKTHLVADVDDINIDVKK
VSILPLEVIVRNIAAGSFSRRMGVKEGTPFKKPIVEFSYKDDSLGDPFINDDYAREMNAATEEECVFLKNQARIVNDVLI
DFFKQVGLTLVDFKIEFGRLAEDPSQIVLADEISPDTCRLWDIKTGEKMDKDRFRQDLGNVMEAYEEVLSRLQNKA
>Mature_236_residues
MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKGRMNKAITLVIYRMLESKGVKTHLVADVDDINIDVKK
VSILPLEVIVRNIAAGSFSRRMGVKEGTPFKKPIVEFSYKDDSLGDPFINDDYAREMNAATEEECVFLKNQARIVNDVLI
DFFKQVGLTLVDFKIEFGRLAEDPSQIVLADEISPDTCRLWDIKTGEKMDKDRFRQDLGNVMEAYEEVLSRLQNKA

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=223, Percent_Identity=34.0807174887892, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI5453539, Length=223, Percent_Identity=34.0807174887892, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI119220559, Length=230, Percent_Identity=33.0434782608696, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1788820, Length=229, Percent_Identity=42.3580786026201, Blast_Score=195, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17531275, Length=209, Percent_Identity=29.6650717703349, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18860083, Length=223, Percent_Identity=30.4932735426009, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583917, Length=251, Percent_Identity=28.6852589641434, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_AKKM8 (B2UP00)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877151.1
- ProteinModelPortal:   B2UP00
- SMR:   B2UP00
- GeneID:   6274982
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0532
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 26855; Mature: 26855

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKGRMNKAITLVIYRMLES
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
KGVKTHLVADVDDINIDVKKVSILPLEVIVRNIAAGSFSRRMGVKEGTPFKKPIVEFSYK
CCCEEEEEECCCCCCEEEEEEEECHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECC
DDSLGDPFINDDYAREMNAATEEECVFLKNQARIVNDVLIDFFKQVGLTLVDFKIEFGRL
CCCCCCCCCCCHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
AEDPSQIVLADEISPDTCRLWDIKTGEKMDKDRFRQDLGNVMEAYEEVLSRLQNKA
CCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKGRMNKAITLVIYRMLES
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
KGVKTHLVADVDDINIDVKKVSILPLEVIVRNIAAGSFSRRMGVKEGTPFKKPIVEFSYK
CCCEEEEEECCCCCCEEEEEEEECHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECC
DDSLGDPFINDDYAREMNAATEEECVFLKNQARIVNDVLIDFFKQVGLTLVDFKIEFGRL
CCCCCCCCCCCHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
AEDPSQIVLADEISPDTCRLWDIKTGEKMDKDRFRQDLGNVMEAYEEVLSRLQNKA
CCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA